A novel acid-stable, acid-active β-galactosidase potentially suited to the alleviation of lactose intolerance

Applied Microbiology and Biotechnology

Volumn 86, Issue 2, 2010, Pages 517-524

  O'Connell, Shane ^a   Walsh, Gary ^b  

^a Munster Technological University   (Ireland)

^b UNIVERSITY OF LIMERICK   (Ireland)

Author keywords

galactosidase; Aspergillus niger van Tiegh; Lactase; Lactose intolerance; Two segment capsule

Indexed keywords

ACTIVITY LEVELS; ASPERGILLUS NIGER; CAPSULE SYSTEM; CHROMATOFOCUSING; DELIVERY SYSTEMS; DIGESTIVE TRACT; EXTRACELLULAR; GALACTOSIDASES; GASTRIC CONDITIONS; GEL FILTRATION; GLYCOSYLATED; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IN-VITRO; ISO-ELECTRIC POINTS; KLUYVEROMYCES MARXIANUS; NIGER; PER UNIT; PH OPTIMA; PHYSICOCHEMICAL PROPERTY; PROTEIN DATABASE; SEQUENCE DATA; SIMILARITY SEARCHING; SMALL INTESTINE;

ACIDS; CHROMATOGRAPHIC ANALYSIS; DIGESTIVE SYSTEM; HYDROPHOBICITY; ION EXCHANGE; MASS SPECTROMETRY; SUGARS;

ENZYMES;

ACID; BETA GALACTOSIDASE; GLYCOSYLATED PROTEIN; LACTASE; LACTOSE; MONOMER;

CHROMATOGRAPHY; ENZYME ACTIVITY; FILTRATION; FUNGUS; ION EXCHANGE; MASS SPECTROMETRY;

ARTICLE; ASPERGILLUS NIGER; CONTROLLED STUDY; DIGESTIVE SYSTEM; ENZYME STABILITY; HYDROPHOBICITY; ISOELECTRIC POINT; KLUYVEROMYCES; KLUYVEROMYCES MARXIANUS; LACTOSE INTOLERANCE; MOLECULAR INTERACTION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PROTEIN DATABASE; SEQUENCE HOMOLOGY; SIMULATION; SMALL INTESTINE; STOMACH; THERMOSTABILITY;

ASPERGILLUS NIGER; BETA-GALACTOSIDASE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ENZYME STABILITY; FUNGAL PROTEINS; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; KLUYVEROMYCES; LACTOSE; LACTOSE INTOLERANCE; MASS SPECTROMETRY; MOLECULAR WEIGHT;

ASPERGILLUS NIGER; KLUYVEROMYCES MARXIANUS;

EID: 77249089742     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2270-7     Document Type: Article

References (43)

1. M Bradford 1976 A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principal of protein dye binding Anal Biochem 72 248 254 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D
2. S Chakraborti R Sani U Banerjee R Sobti 2000 Purification and characterisation of a novel β-galactosidase from Bacillus sp MTCC3088 J Ind Microbiol Biotech 24 58 63 10.1038/sj.jim.2900770 1:CAS:528: DC%2BD3cXhtlyhsrk%3D
3. P Christakopoulos B Macris D Kekos 1990 Exceptionally thermostable α-galactosidase and β -galactosidase from Aspergillus niger seperated in one step Process Biochem 25 210 212 1:CAS:528:DyaK38XmtVOltb0%3D
4. M Diaz A Pedregosa J Lucas S Torralba I Monistrol F Laborda 1996 Purification and properties of β-galactosidase from Aspergillus nidulans Microbiologia SEM 12 585 592 1:CAS:528:DyaK2sXitFaltrs%3D
5. J Dipalma M Collins 1989 Enzyme replacement for lactose malabsorption using a β-D-galactosidase Gastroenterol 11 290 293 1:STN:280: DyaL1Mzislyksw%3D%3D
6. T Finocchiaro N Olson T Richardson 1980 Use of immobilised lactase in milk systems Adv Biochem Eng 15 71 88 1:CAS:528:DyaL3cXkt1Cnu7Y%3D
7. L Fischer C Scheckermann F Wagner 1995 Purification and characterisation of a thermotolerant β-galactosidase from Thermomyces lanuginosus Appl Environ Microbiol 61 1497 1501 1:CAS:528:DyaK2MXksl2lsbo%3D
8. K Gao T Mitsui K Fujiki H Ishiguro T Kondo 2002 Effect of lactase preparations in asympthomatic individuals with lactase deficiency-gastric digestion of lactose and breath hydrogen analysis Nagoya J Med Sci 65 21 28 1:CAS:528:DC%2BD38Xlt1yrtLY%3D
9. J Gaska 1990 Treatment of lactose intolerance Am Drug 202 36 43
10. V Geskas M Lopez-Levia 1985 Hydrolysis of lactose: a literature review Process Biochem 20 2 12
11. R Gonzalez P Monsan 1991 Purification and some characteristics of β-galactosidase from Aspergillus fonsecaeus Enzyme Microb Technol 13 349 352 10.1016/0141-0229(91)90156-5 1:CAS:528:DyaK3MXitVaht7o%3D
12. N Greenberg R Mahoney 1981 Immobilisation of lactase (β- galactosidase) for use in dairy processing: a review Process Biochem 16 2 8 1:CAS:528:DyaL3MXksVSrtbk%3D
13. Holsinger V (1988) Lactose. In: Wong P (ed) Fundamentals of dairy chemistry, 3rd edn. Van Nostrand Reihold Co, New York, pp 279-341
14. F Ingels S Deferme E Destexhe M Oth G Van den Mooter P Augustijns 2002 Simulated intestinal fluid as transport medium in the Caco-2 cell culture model Int J Pharm 232 183 192 10.1016/S0378-5173(01)00897-3 1:CAS:528: DC%2BD38XjvFCjtA%3D%3D (Pubitemid 34076764)
15. D Kanabar M Randhawa P Clayton 2001 Improvement of symptoms in infant colic following reduction of lactose load with lactase J Hum Nutr Diet 14 359 363 10.1046/j.1365-277X.2001.00304.x 1:STN:280:DC%2BD387nt1ClsA%3D%3D (Pubitemid 33014889)
16. U Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 10.1038/227680a0 1:CAS:528:DC%2BD3MXlsFags7s%3D
17. Lehmann K (2001) Practical course in film coating of pharmaceutical dosage forms with Eudragit. Pharma Polymers, Darmstadt
18. E Letunova A Tikhomirova S Shiian V Markin A Khorlin 1981 Purification and properties of β-galactosidase from Alternaria tenius Biokemia 46 911 919 1:CAS:528:DyaL3MXktlOrur0%3D
19. M Lin J Dipalma M Martini C Gross S Harlander D Savaiano 1993 Comparative effects of exogenous lactase (β-galactosidase) preparations on in vivo lactose digestion Dig Dis Sci 38 2022 2027 10.1007/BF01297079 1:CAS:528:DyaK2cXhsVertb8%3D (Pubitemid 23336565)
20. A Mackey T Haystead W Pearson 2002 Getting more from less-algorithms for rapid protein identification with multiple short peptide sequences Mol Cell Proteomics 1 139 147 10.1074/mcp.M100004-MCP200 1:CAS:528:DC%2BD38Xis1Oitrk%3D
21. P Manzanares L de Graff J Visser 1998 Characterisation of galactosidases from Aspergillus niger: purification of a novel α-galactosidase activity Enzyme Microb Technol 22 383 390 10.1016/S0141-0229(97)00207-X 1:CAS:528:DyaK1cXitlygtro%3D (Pubitemid 28177065)
22. M Medow L Thek S Newman M Berezin M Glassman S Schwarz 1990 β-galactosidase tablets in the treatment of lactose intolerance in pediatrics Am J Dis Child 114 1261 1264
23. Z Nagy T Kiss A Szentirmai S Biro 2001 β-galactosidase of Penicillium chrysogenum: production, purification and characterisation of the enzyme Protein Expr Purif 21 24 29 10.1006/prep.2000.1344 1:CAS:528: DC%2BD3MXmsFCiuw%3D%3D (Pubitemid 32614492)
24. H Nevalinen 1981 Induction, isolation, and characterisation of Aspergillus niger mutant strains producing elevated levels of β-galactosidase Appl Environ Microbiol 41 593 596
25. S O'Connell G Walsh 2006 Physicochemical characteristics of commercial lactases relevant to their application in the alleviation of lactose intolerance Appl Biochem Biotechnol 134 179 191 10.1385/ABAB:134:2:179 (Pubitemid 44337214)
26. S O'Connell G Walsh 2007 Purification and properties of a β-galactosidase with potential application as a digestive supplement Appl Biochem Biotechnol 141 1 14 10.1007/s12010-007-9206-4 (Pubitemid 47186936)
27. S O'Connell G Walsh 2008 Application relevant studies of fungal β-galactosidases with potential application in the alleviation of lactose intolerance Appl Biochem Biotechnol 149 129 138 10.1007/s12010-007-8098-7
28. S Ogushi T Yoshimoto D Tsuru 1980 Purification and comparison of two types of β-galactosidases from Aspergillus oryzae J Ferment Technol 58 115 122 1:CAS:528:DyaL3cXktVartrk%3D
29. Y Park M De Santi G Pastore 1979 Production and characterisation of β-galactosidase from Aspergillus oryzae J Food Sci 44 100 103 10.1111/j.1365-2621.1979.tb10016.x 1:CAS:528:DyaE1MXntVGktg%3D%3D
30. D Perkins D Pappin D Creasy J Cottrell 1999 Probability-based protein identification by searching sequence databases using mass spectrometry data Electrophoresis 20 3551 3567 10.1002/(SICI)1522-2683(19991201)20:18<3551:: AID-ELPS3551>3.0.CO;2-2 1:CAS:528:DC%2BD3cXhtF2ntw%3D%3D
31. F Ramirez K Lee D Graham 1994 All lactase preparations are not the same: results of a prospective, randomised, placebo-controlled trial Am J Gastroenterol 89 566 570 1:STN:280:DyaK2c7pslWlsw%3D%3D
32. I Rasouli P Kulkarni 1994 Enhancement of β-galactosidase productivity of Aspergillus niger NCIM-616 J Appl Bacteriol 77 359 361 1:CAS:528:DyaK2MXitVWgt7w%3D
33. S Sanders K Tolman D Reitberg 1992 Effect of a single dose of lactase on symptoms and expired hydrogen after lactose challenge in lactose-intolerant subjects Clin Pharm 11 533 538 1:STN:280:DyaK383ovFWluw%3D%3D
34. SA Shaikh J Khire M Khan 1999 Characterisation of a thermostable extracellular β-galactosidase from a thermophilic fungus Rhizomucor sp Biochim Biophys Acta 1472 314 322 1:CAS:528:DyaK1MXnsFSrtrw%3D (Pubitemid 29486542)
35. A Shevchenko S Sunyaev A Loboda A Shevchenko P Bork W Ens K Standing 2001 Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching Anal Chem 73 1917 1926 10.1021/ac0013709 1:CAS:528:DC%2BD3MXitVyrsr4%3D (Pubitemid 32862395)
36. I Shrier J Szilagyi A Correa 2008 Impact of lactose containing foods and the genetics of lactase on diseases: an analytical review of population data Nutr Cancer 60 292 300 10.1080/01635580701745301 (Pubitemid 351601365)
37. D Sykes S Abbas J Barlow K Matta 1983 Substrate-specificity and other properties of the beta-D-galactosidase from Aspergillus niger Carboh Res 116 127 138 10.1016/S0008-6215(00)90960-1 1:CAS:528:DyaL3sXktVahsLs%3D
38. Y Tanaka A Kagamiishi A Kiuchi T Horiuchi 1975 Purification and properties of β-galactosidase from Aspergillus oryzae J Biochem (Tokyo) 77 241 247 1:CAS:528:DyaE2MXhtVyjsbg%3D
39. W van Casteren M Eimermann L Van den Broek J Vincken H Schols H A Voragen 2000 Purification and characterisation of a β-galactosidase from Aspergillus aculeatus with activity towards (modified) exopolysaccharides from Lactococcus lactis subsp. cremoris B39 and B891 Carbohydr Res 329 75 85 10.1016/S0008-6215(00)00152-X (Pubitemid 30797972)
40. E Van Griethuysen-Dilber E Flaschel A Renken 1988 Process development for the hydrolysis of lactose in whey by immobilised lactase of Aspergillus oryzae Process Biochem 23 55 58
41. T Vesa P Marteau R Korpela 2000 Lactose intolerance J Am Coll Nutr 19 165s 175s 1:STN:280:DC%2BD3c3itVyqsw%3D%3D
42. F Widmer J Leuba 1979 β-galactosidase from Aspergillus niger. Separation and characterisation of three multiple forms Eur J Biochem 100 559 567 10.1111/j.1432-1033.1979.tb04202.x 1:CAS:528:DyaL3cXit1Sg (Pubitemid 10165897)
43. K Xenos S Kyroundis A Anagnostidis P Papastathopoulos 1998 Treatment of lactose intolerance with exogenous β-D-galactosidase in Pellet Form Eur J Drug Metab Pharmacokinet 23 350 355 1:CAS:528:DyaK1cXlvVeitrc%3D