mRNA stability and antibody production in CHO cells: Improvement through gene optimization

Biotechnology Journal

Volumn 5, Issue 4, 2010, Pages 393-401

  Hung, Finn ^a   Deng, Liang ^a   Ravnikar, Paula ^b   Condon, Russ ^a   Li, Benson ^a   Do, Lien ^a   Saha, Deba ^a   Tsao, Yung Shyeng ^a   Merchant, Ankit ^a   Liu, Zhong ^a   Shi, Shuangping ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b Invitrogen Corporation   (United States)

Author keywords

Chinese hamster ovary cells; Gene optimization; Manufacturing cell line; Monoclonal antibody; Transfection

Indexed keywords

ANTIBODY EXPRESSION; ANTIBODY PRODUCTION; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; EXPRESSION VECTORS; GENE CODING SEQUENCES; HEAVY AND LIGHT CHAINS; HEAVY CHAIN; MAMMALIAN CELL CULTURE; MANUFACTURING CELLS; MRNA LEVEL; MRNA STABILITY; PRODUCTION OUTPUTS; RECOMBINANT PROTEIN; STABLE CLONES; THERAPEUTIC ANTIBODIES; THERAPEUTIC PROTEIN; TRANSFECTED CELLS; TRANSIENT TRANSFECTION;

CELLS; FLEXIBLE MANUFACTURING SYSTEMS; GENE EXPRESSION; MAMMALS; MONOCLONAL ANTIBODIES; NUCLEIC ACIDS; OPTIMIZATION; PROTEINS; SURFACE PLASMON RESONANCE;

CELL CULTURE;

ANTIBODY; MESSENGER RNA;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CULTURE; CELL LINE; CHO CELL; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSFECTION; HEAVY CHAIN; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; RNA STABILITY; TRANSIENT TRANSFECTION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; GENETIC ENHANCEMENT; PROTEIN ENGINEERING; RNA STABILITY; RNA, MESSENGER;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77950666813     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200900192     Document Type: Article

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