Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters

Journal of Biotechnology

Volumn 128, Issue 4, 2007, Pages 716-725

  Lattenmayer, Christine ^a   Trummer, Evelyn ^a   Schriebl, Kornelia ^a   Vorauer Uhl, Karola ^b   Mueller, Dethardt ^b   Katinger, Hermann ^b,c   Kunert, Renate ^b  

^a Austrian Center of Biopharmaceutical Technology   (Austria)

^b UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^c POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH   (Austria)

Author keywords

CHO cells; Gene copy number; Genetic characterisation; Genetic parameters; Quantitative PCR; Taqman

Indexed keywords

CHEMICAL ANALYSIS; GENES; GENETIC ENGINEERING; PROTEINS; RNA;

GENE COPY NUMBER; GENETIC CHARACTERISATION; GENETIC PARAMETERS; QUANTITATIVE PCR;

CELL CULTURE;

ERYTHROPOIETIN; FC RECEPTOR; HYBRID PROTEIN; IMMUNOGLOBULIN G1; MESSENGER RNA; METHOTREXATE;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL CLONE; CELL GROWTH; CHO CELL; CHROMOSOMAL INSTABILITY; CORRELATION ANALYSIS; GENE NUMBER; GENETIC ANALYSIS; GENETIC PARAMETERS; GROWTH RATE; INTERMETHOD COMPARISON; KARYOTYPE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; QUANTITATIVE ASSAY;

ANIMALS; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CELL ADHESION MOLECULES; CHO CELLS; CRICETINAE; CRICETULUS; ERYTHROPOIETIN; GENE DOSAGE; MEMBRANE PROTEINS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER;

EID: 33847374097     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.12.016     Document Type: Article

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