Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT 7(a) receptor

FEBS Journal

Volumn 279, Issue 11, 2012, Pages 1994-2003

  Gellynck, Evelien ^a   Andressen, Kjetil W ^b   Lintermans, Béatrice ^a   Haegeman, Guy ^a   Levy, Finn O ^b   Vanhoenacker, Peter ^a,c   Van Craenenbroeck, Kathleen ^a  

^a GHENT UNIVERSITY   (Belgium)

^b UNIVERSITY OF OSLO   (Norway)

^c ActoGeniX NV   (Belgium)

Author keywords

G protein coupled receptor; membrane trafficking; N glycosylation; serotonin receptor

Indexed keywords

ADENYLATE CYCLASE; ASPARAGINE; MUTANT PROTEIN; SEROTONIN 7 RECEPTOR; SEROTONIN 7A RECEPTOR; TUNICAMYCIN; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE; CELL SURFACE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; LIGAND BINDING; MOLECULAR WEIGHT; MUTATION; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; RECEPTOR AFFINITY; RECEPTOR DENSITY; TRANSIENT EXPRESSION; WESTERN BLOTTING; WILD TYPE;

ADENYLATE CYCLASE; ASPARAGINE; GLUTAMINE; GLYCOSYLATION; HEK293 CELLS; HUMANS; MUTATION; PLASMIDS; PROTEIN BINDING; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; SEROTONIN; TRANSFECTION;

EID: 84861190674     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08581.x     Document Type: Article

References (39)

1. Barnes NM, &, Sharp T, (1999) A review of central 5-HT receptors and their function. Neuropharmacology 38, 1083-1152. (Pubitemid 29292403)
2. 7) receptor isoforms produced by alternative splicing. Neuropharmacology 37, 1621-1632.
3. 7 receptor splice variants from mouse brain. Gene 426, 23-31.
4. 4) receptors and central nervous system function: an update. Prog Drug Res 49, 9-24. (Pubitemid 27473750)
5. 7 receptors activate the mitogen activated protein kinase extracellular signal related kinase in cultured rat hippocampal neurons. Neuroscience 102, 361-367. (Pubitemid 32126490)
6. 7(a). J Biol Chem 278, 3098-3104. (Pubitemid 36801219)
7. 12-protein to regulate gene transcription and neuronal morphology. J Neurosci 25, 7821-7830. (Pubitemid 41208654)
8. Markkanen PM, &, Petaja-Repo UE, (2008) N-glycan-mediated quality control in the endoplasmic reticulum is required for the expression of correctly folded δ opioid receptors at the cell surface. J Biol Chem 283, 29086-29098.
9. Lederkremer GZ, &, Glickman MH, (2005) A window of opportunity: timing protein degradation by trimming of sugars and ubiquitins. Trends Biochem Sci 30, 297-303. (Pubitemid 40799047)
10. 2 receptor sialylation and N-glycosylation participate with disulfide bonding in surface receptor dimerization. Biochemistry 45, 2699-2707.
11. Couvineau A, Fabre C, Gaudin P, Maoret JJ, &, Laburthe M, (1996) Mutagenesis of N-glycosylation sites in the human vasoactive intestinal peptide 1 receptor. Evidence that asparagine 58 or 69 is crucial for correct delivery of the receptor to plasma membrane. Biochemistry 35, 1745-1752. (Pubitemid 26062623)
12. 1a angiotensin II receptor delivery to the plasma membrane. Biochem J 339, 397-405. (Pubitemid 29209971)
13. Yan K, Khoshnoodi J, Ruotsalainen V, &, Tryggvason K, (2002) N-linked glycosylation is critical for the plasma membrane localization of nephrin. J Am Soc Nephrol 13, 1385-1389. (Pubitemid 34441421)
14. 3β for correct sorting to the plasma membrane but not for correct folding. Biochem J 350, 839-847.
15. 1 receptor. Biochemistry 38, 8621-8627.
16. 5A receptor. Neurochem Int 52, 419-425.
17. Michineau S, Muller L, Pizard A, Alhenc-Gelas F, &, Rajerison RM, (2004) N-linked glycosylation of the human bradykinin B2 receptor is required for optimal cell-surface expression and coupling. Biol Chem 385, 49-57. (Pubitemid 38240294)
18. Kelley LP, &, Kinsella BT, (2003) The role of N-linked glycosylation in determining the surface expression, G protein interaction and effector coupling of the alpha (alpha) isoform of the human thromboxane A(2) receptor. Biochim Biophys Acta 1621, 192-203. (Pubitemid 36515099)
19. 1, dopamine receptors in transfected mammalian cells. Mol Pharmacol 56, 1071-1078.
20. 1a vasopressin receptor. Biochem Biophys Res Commun 286, 707-713.
21. 2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function. J Biol Chem 265, 20887-20892. (Pubitemid 120014093)
22. 2 receptor by site-directed mutagenesis: N-glycosylation is not vital for its action. Biochem J 310, 553-558.
23. 4 receptor biogenesis. J Biol Chem 280, 19350-19357. (Pubitemid 41379642)
24. 7 receptor isoforms and PLAC-24/eIF3k. Cell Signal 19, 278-288. (Pubitemid 44969977)
25. 2 dopamine receptors and their chimaeras: analysis by subtype-specific antibodies. Biochem Biophys Res Commun 205, 1460-1466.
26. 7 receptors: radioligand binding and functional evidence for GPCR homodimer protomer interactions. Psychopharmacology 212, 687-697.
27. Trombetta ES, &, Parodi AJ, (2003) Quality control and protein folding in the secretory pathway. Annu Rev Cell Dev Biol 19, 649-676. (Pubitemid 37487364)
28. 2C receptors as glycoproteins containing N-linked oligosaccharides. Brain Res Mol Brain Res 33, 311-318.
29. Abramowski D, &, Staufenbiel M, (1995) Identification of the 5-hydroxytryptamine2C receptor as a 60-kDa N-glycosylated protein in choroid plexus and hippocampus. J Neurochem 65, 782-790.
30. Contesse V, Lenglet S, Grumolato L, Anouar Y, Lihrmann I, Lefebvre H, Delarue C, &, Vaudry H, (1999) Pharmacological and molecular characterization of 5-hydroxytryptamine(7) receptors in the rat adrenal gland. Mol Pharmacol 56, 552-561. (Pubitemid 29406882)
31. Kaushal S, Ridge KD, &, Khorana HG, (1994) Structure and function in rhodopsin: the role of asparagine-linked glycosylation. Proc Natl Acad Sci USA 91, 4024-4028. (Pubitemid 24139565)
32. A. J Biol Chem 275, 24752-24759. (Pubitemid 30626578)
33. Ray K, Clapp P, Goldsmith PK, &, Spiegel AM, (1998) Identification of the sites of N-linked glycosylation on the human calcium receptor and assessment of their role in cell surface expression and signal transduction. J Biol Chem 273, 34558-34567. (Pubitemid 29008938)
34. 7(a) receptor and Gs. Receptors Channels 9, 107-116. (Pubitemid 36553039)
35. 7 receptor splice variants: a comparative characterization of their pharmacology, function and distribution. Naunyn Schmiedebergs Arch Pharmacol 363, 620-632. (Pubitemid 32477270)
36. 4(b) receptors have nearly identical pharmacology and are both expressed in human atrium and ventricle. Naunyn Schmiedebergs Arch Pharmacol 363, 146-160. (Pubitemid 32200322)
37. Salomon Y, Londos C, &, Rodbell M, (1974) A highly sensitive adenylate cyclase assay. Anal Biochem 58, 541-548.
38. Bockaert J, Hunzicker-Dunn M, &, Birnbaumer L, (1976) Hormone-stimulated desensitization of hormone-dependent adenylyl cyclase. Dual action of luteninizing hormone on pig graafian follicle membranes. J Biol Chem 251, 2653-2663.
39. 7 serotonin receptor. Eur J Pharmacol 532, 1-10.