Identification and functional significance of N-glycosylation of the 5-ht5A receptor

Neurochemistry International

Volumn 52, Issue 3, 2008, Pages 419-425

  Dutton, Alice C ^a   Massoura, Andrew N ^a   Dover, Terri J ^a   Andrews, Nick A ^b   Barnes, Nicholas M ^a  

^a UNIVERSITY DEPARTMENT OF MEDICINE   (United Kingdom)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

h5 ht5A receptor; Membrane trafficking; N Glycosylation; Serotonin

Indexed keywords

ASPARAGINE; SEROTONIN 5A RECEPTOR; TUNICAMYCIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE MUTATION; GLYCOSYLATION; IMMUNOCYTOCHEMISTRY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION;

ANIMALS; BRAIN; CERCOPITHECUS AETHIOPS; COS CELLS; GLYCOSYLATION; HUMANS; MOLECULAR WEIGHT; MUTATION; NEURONS; NITROGEN; PROTEIN ISOFORMS; RECEPTORS, SEROTONIN; SEROTONIN; SYNAPTIC TRANSMISSION; TRANSFECTION; TUNICAMYCIN;

EID: 38049063439     PISSN: 01970186     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neuint.2007.07.020     Document Type: Article

References (40)

1. Barnes N.M., and Sharp T. A review of central 5-HT receptors and their function. Neuropharmacology 38 8 (1999) 1083-1152
2. Birkett J.T., Arranz M.J., Munro J., Osbourn S., Kerwin R.W., and Collier D.A. Association analysis of the 5-HT5A gene in depression, psychosis and antipsychotic response. Neuroreport 11 9 (2000) 2017-2020
3. Boer U., Neuschafer-Rube F., Moller U., and Puschel G.P. Requirement of N-glycosylation of the prostaglandin E2 receptor EP3beta for correct sorting to the plasma membrane but not for correct folding. Biochem. J. 350 Pt 3 (2000) 839-847
4. Corbett D.F., Heightman T.D., Moss S.F., Bromidge S.M., Coggon S.A., Longley M.J., Roa A.M., Williams J.A., and Thomas D.R. Discovery of a potent and selective 5-ht5A receptor antagonist by high-throughput chemistry. Bioorg. Med. Chem. Lett. 15 18 (2005) 4014-4018
5. Couvineau A., Fabre C., Gaudin P., Maoret J.-J., and Laburthe M. Mutagenesis of N-glycosylation sites in the human vasoactive intestinal peptide 1 receptor evidence that asparagine 58 or 69 is crucial for correct delivery of the receptor to plasma membrane. Biochemistry 35 (1996) 1745-1752
6. Deriu D., Gassmann M., Firbank S., Ristig D., Lampert C., Mosbacher J., Froestl W., Kaupmann K., Bettler B., and Grutter M.G. Determination of the minimal functional ligand-binding domain of the GABAB1b receptor. Biochem. J. 386 Pt 3 (2005) 423-431
7. Dietz B.M., Mahady G.B., Pauli G.F., and Farnsworth N.R. Valerian extract and valerenic acid are partial agonists of the 5-HT5a receptor in vitro. Brain Res. Mol. Brain Res. 138 2 (2005) 191-197
8. Doly S., Fischer J., Brisorgueil M.J., Verge D., and Conrath M. 5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature. J. Comp. Neurol. 476 4 (2004) 316-329
9. Dubertret C., Hanoun N., Ades J., Hamon M., and Gorwood P. Family-based association studies between 5-HT5A receptor gene and schizophrenia. J. Psychiatr. Res. 38 4 (2004) 371-376
10. Duvernay M.T., Filipeanu C.M., and Wu G. The regulatory mechanisms of export trafficking of G protein-coupled receptors. Cell. Signal. 12 (2005) 1457-1465
11. Erlander M.G., Lovenberg T.W., Baron B.M., de Lecea L., Danielson P.E., Racke M., Slone A.L., Siegel B.W., Foye P.E., and Cannon K. Two members of a distinct subfamily of 5-hydroxytryptamine receptors differentially expressed in rat brain. Proc. Natl. Acad. Sci. USA 90 8 (1993) 3452-3456
12. Francken B.J., Jurzak M., Vanhauwe J.F., Luyten W.H., and Leysen J.E. The human 5-ht5A receptor couples to Gi/Go proteins and inhibits adenylate cyclase in HEK 293 cells. Eur. J. Pharmacol. 361 2-3 (1998) 299-309
13. Fukushima Y., Oka Y., Saitoh T., Katagiri H., Asano T., Matsuhashi N., Takata K., van Breda E., Yazaki Y., and Sugano K. Structural and functional analysis of the canine histamine H2 receptor by site-directed mutagenesis: N-glycosylation is not vital for its action. Biochem. J. 310 Pt 2 (1995) 553-558
14. Grailhe R., Waeber C., Dulawa S.C., Hornung J.P., Zhuang X., Brunner D., Geyer M.A., and Hen R. Increased exploratory activity and altered response to LSD in mice lacking the 5-HT(5A) receptor. Neuron 22 3 (1999) 581-591
15. Grailhe R., Grabtree G.W., and Hen R. Human 5-HT(5) receptors: the 5-HT(5A) receptor is functional but the 5-HT(5B) receptor was lost during mammalian evolution. Eur. J. Pharmacol. 418 3 (2001) 157-167
16. Hurley P.T., McMahon R.A., Fanning P., O'Boyle K.M., Rogers M., and Martin F. Functional coupling of a recombinant human 5-HT5A receptor to G-proteins in HEK-293 cells. Brit. J. Pharmacol. 124 6 (1998) 1238-1244
17. Hwan Lee K., Ahn J.I., Yu D.H., Jeong H.S., Lee S.H., Kim K.S., Chung I.Y., Kim J.H., and Lee Y.S. Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor. Biochem. Biophys. Res. Commun. 286 (2001) 707-713
18. Kelley L.P., and Kinsella B.T. The role of N-linked glycosylation in determining the surface expression G protein interaction and effector coupling of the alpha (alpha) isoform of the human thromboxane A(2) receptor. Biochim. Biophys. Acta 1621 2 (2003) 192-203
19. Khorana N., Smith C., Herrick-Davis K., Purohit A., Teitler M., Grella B., Dukat M., and Glennon R.A. Binding of tetrahydrocarboline derivatives at human 5-HT5A receptors. J. Med. Chem. 46 18 (2003) 3930-3937
20. Lanctot P.M., Leclerc P.C., Escher E., Leduc R., and Guillemette G. Role of N-glycosylation in the expression and functional properties of human AT1 receptor. Biochemistry 38 27 (1999) 8621-8627
21. Lederkremer G.Z., and Glickman M.H. A window of opportunity: timing protein degradation by trimming of sugars and ubiquitins. Trends Biochem. Sci. 30 6 (2005) 297-303
22. Lee K.H., Ahn J.I., Yu D.H., Jeong H.S., Lee S.H., Kim K.S., Chung I.Y., Kim J.H., and Lee Y.S. Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor. Biochem. Biophys. Res. Commun. 286 4 (2001) 707-713
23. Matthes H., Boschert U., Amlaiky N., Grailhe R., Plassat J.L., Muscatelli F., Mattei M.G., and Hen R. Mouse 5-hydroxytryptamine5A and 5-hydroxytryptamine5B receptors define a new family of serotonin receptors: cloning, functional expression, and chromosomal localization. Mol. Pharmacol. 43 3 (1993) 313-319
24. Mialet-Perez J., Green S.A., Miller W.E., and Liggett S.B. A primate-dominant third glycosylation site of the beta2-adrenergic receptor routes receptors to degradation during agonist regulation. J. Biol. Chem. 279 37 (2004) 38603-38607
25. Noda M., Yasuda S., Okada M., Higashida H., Shimada A., Iwata N., Ozaki N., Nishikawa K., Shirasawa S., Uchida M., Aoki S., and Wada K. Recombinant human serotonin 5A receptors stably expressed in C6 glioma cells couple to multiple signal transduction pathways. J. Neurochem. 84 2 (2003) 222-232
26. Oliver K.R., Kinsey A.M., Wainwright A., and Sirinathsinghji D.J. Localization of 5-ht(5A) receptor-like immunoreactivity in the rat brain. Brain Res. 867 1-2 (2000) 131-142
27. Parodi A.J. Protein glucosylation and its role in protein folding. Ann. Rev. Biochem. 69 (2000) 69-93
28. Pasqualetti M., Ori M., Marazziti D., Castagna M., and Nardi I. Distribution of 5-HT2c and 5-ht5a receptor mRNA in human brain. Ann. N Y Acad. Sci. 861 (1998) 245
29. Petaja-Repo U.E., Hogue M., Laperriere A., Walker P., and Bouvier M. Export from the endoplasmic reticulum represents the limiting step in the maturation and cell surface expression of the human delta opioid receptor. J. Biol. Chem. 275 18 (2000) 13727-13736
30. Plassat J.L., Boschert U., Amlaiky N., and Hen R. The mouse 5HT5 receptor reveals a remarkable heterogeneity within the 5HT1D receptor family. EMBO J. 11 13 (1992) 4779-4786
31. Rees S., den Daas I., Foord S., Goodson S., Bull D., Kilpatrick G., and Lee M. Cloning and characterisation of the human 5-HT5A serotonin receptor. FEBS Lett. 355 3 (1994) 242-246
32. Sadeghi H.M., Innamorati G., and Birnbaumer M. Maturation of receptor proteins in eukaryotic expression systems. J. Recept. Signal Transduct. Res. 17 1-3 (1997) 433-445
33. Sawutz D.G., Lanier S.M., Warren C.D., and Graham R.M. Glycosylation of the mammalian alpha 1-adrenergic receptor by complex type N-linked oligosaccharides. Mol. Pharmacol. 32 5 (1987) 565-571
34. Sprouse J., Reynolds L., Braselton J., and Schmidt A. Serotonin-induced phase advances of SCN neuronal firing in vitro: a possible role for 5-HT5A receptors?. Synapse 54 2 (2004) 111-118
35. Thomas D.R. 5-ht5A receptors as a therapeutic target. Pharmacol. Therapeut. 111 3 (2006) 707-714
36. Thomas D.R., Larminie C.G., Lyons H.R., Fosberry A., Hill M.J., and Hayes P.D. Cloning and pharmacological characterisation of the guinea pig 5-ht5A receptor. Eur. J. Pharmacol. 494 2-3 (2004) 91-99
37. Thomas D.R., Soffin E.M., Roberts C., Kew J.N., de la Flor R.M., Dawson L.A., Fry V.A., Coggon S.A., Faedo S., Hayes P.D., Corbett D.F., Davies C.H., and Hagan J.J. SB-699551-A (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4′-{[(2-phenylethyl)amino]methyl}-4-biphenylyl)methyl]propanamide dihydrochloride), a novel 5-ht5A receptor-selective antagonist, enhances 5-HT neuronal function: Evidence for an autoreceptor role for the 5-ht5A receptor in guinea pig brain. Neuropharmacology 51 3 (2006) 566-577
38. van Koppen C.J., and Nathanson N.M. Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function. J. Biol. Chem. 265 34 (1990) 20887-20892
39. Wheatley M., and Hawtin S.R. Glycosylation of G-protein-coupled receptors for hormones central to normal reproductive functioning: its occurrence and role. Hum. Reproduct. Update 5 4 (1999) 356-364
40. Xu C., Giuliano F., Sun X.Q., Brisorgueil M.J., Leclerc P., Verge D., and Conrath M. Serotonin 5-HT2A and 5-HT5A receptors are expressed by different motoneuron populations in rat Onuf's nucleus. J. Comp. Neurol. 502 4 (2007) 620-634