Structure of the SecY Complex Unlocked by a Preprotein Mimic

Cell Reports

Volumn 1, Issue 1, 2012, Pages 21-28

  Hizlan, Dilem ^a   Robson, Alice ^b   Whitehouse, Sarah ^b   Gold, Vicki A ^b   Vonck, Janet ^a   Mills, Deryck ^a   Kühlbrandt, Werner ^a   Collinson, Ian ^b  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; POLYPEPTIDE; PROTEIN PRECURSOR; SECYEG PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; MOLECULAR IMAGING; MOLECULAR MIMICRY; PHENOTYPE; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PEPTIDES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN PRECURSORS; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, SECONDARY; PROTEIN TRANSPORT; TRANSCRIPTIONAL ACTIVATION;

BACTERIA (MICROORGANISMS);

EID: 84861167327     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2011.11.003     Document Type: Article

References (32)

1. Bessonneau P., Besson V., Collinson I., Duong F. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure. EMBO J. 2002, 21:995-1003.
2. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 1975, 67:835-851.
3. Bondar A.N., del Val C., Freites J.A., Tobias D.J., White S.H. Dynamics of SecY translocons with translocation-defective mutations. Structure 2010, 18:847-857.
4. Bostina M., Mohsin B., Kühlbrandt W., Collinson I. Atomic model of the E. coli membrane-bound protein translocation complex SecYEG. J. Mol. Biol. 2005, 352:1035-1043.
5. Breyton C., Haase W., Rapoport T.A., Kühlbrandt W., Collinson I. Three-dimensional structure of the bacterial protein-translocation complex SecYEG. Nature 2002, 418:662-665.
6. Brundage L., Hendrick J.P., Schiebel E., Driessen A.J., Wickner W. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 1990, 62:649-657.
7. Cannon K.S., Or E., Clemons W.M., Shibata Y., Rapoport T.A. Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY. J. Cell Biol. 2005, 169:219-225.
8. Collinson I., Breyton C., Duong F., Tziatzios C., Schubert D., Or E., Rapoport T., Kühlbrandt W. Projection structure and oligomeric properties of a bacterial core protein translocase. EMBO J. 2001, 20:2462-2471.
9. Deville K., Gold V.A., Robson A., Whitehouse S., Sessions R.B., Baldwin S.A., Radford S.E., Collinson I. The oligomeric state and arrangement of the active bacterial translocon. J. Biol. Chem. 2011, 286:4659-4669.
10. Duong F. Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase. EMBO J. 2003, 22:4375-4384.
11. Egea P.F., Stroud R.M. Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes. Proc. Natl. Acad. Sci. USA 2010, 107:17182-17187.
12. Emr S.D., Hedgpeth J., Clément J.-M., Silhavy T.J., Hofnung M. Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein. Nature 1980, 285:82-85.
13. Emr S.D., Hanley-Way S., Silhavy T.J. Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 1981, 23:79-88.
14. Flower A.M., Osborne R.S., Silhavy T.J. The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE. EMBO J. 1995, 14:884-893.
15. Frauenfeld J., Gumbart J., Sluis E.O., Funes S., Gartmann M., Beatrix B., Mielke T., Berninghausen O., Becker T., Schulten K., Beckmann R. Cryo-EM structure of the ribosome-SecYE complex in the membrane environment. Nat. Struct. Mol. Biol. 2011, 18:614-621.
16. Gelis I., Bonvin A.M., Keramisanou D., Koukaki M., Gouridis G., Karamanou S., Economou A., Kalodimos C.G. Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR. Cell 2007, 131:756-769.
17. Gold V.A., Robson A., Bao H., Romantsov T., Duong F., Collinson I. The action of cardiolipin on the bacterial translocon. Proc. Natl. Acad. Sci. USA 2010, 107:10044-10049.
18. Gouridis G., Karamanou S., Gelis I., Kalodimos C.G., Economou A. Signal peptides are allosteric activators of the protein translocase. Nature 2009, 462:363-367.
19. Harris C.R., Silhavy T.J. Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking. J. Bacteriol. 1999, 181:3438-3444.
20. Or E., Navon A., Rapoport T.A. Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane. EMBO J. 2002, 21:4470-4479.
21. Osborne R.S., Silhavy T.J. PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains. EMBO J. 1993, 12:3391-3398.
22. Osborne A.R., Rapoport T.A. Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel. Cell 2007, 129:97-110.
23. Park E., Rapoport T.A. Preserving the membrane barrier for small molecules during bacterial protein translocation. Nature 2011, 473:239-242.
24. Plath K., Mothes W., Wilkinson B.M., Stirling C.J., Rapoport T.A. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane. Cell 1998, 94:795-807.
25. Robson A., Booth A.E., Gold V.A., Clarke A.R., Collinson I. A large conformational change couples the ATP binding site of SecA to the SecY protein channel. J. Mol. Biol. 2007, 374:965-976.
26. Robson A., Carr B., Sessions R.B., Collinson I. Synthetic peptides identify a second periplasmic site for the plug of the SecYEG protein translocation complex. FEBS Lett. 2009, 583:207-212.
27. Robson A., Gold V.A., Hodson S., Clarke A.R., Collinson I. Energy transduction in protein transport and the ATP hydrolytic cycle of SecA. Proc. Natl. Acad. Sci. USA 2009, 106:5111-5116.
28. Simon S.M., Blobel G. Signal peptides open protein-conducting channels in E. coli. Cell 1992, 69:677-684.
29. Smith M.A., Clemons W.M., DeMars C.J., Flower A.M. Modeling the effects of prl mutations on the Escherichia coli SecY complex. J. Bacteriol. 2005, 187:6454-6465.
30. Tam P.C., Maillard A.P., Chan K.K., Duong F. Investigating the SecY plug movement at the SecYEG translocation channel. EMBO J. 2005, 24:3380-3388.
31. Van den Berg B., Clemons W.M., Collinson I., Modis Y., Hartmann E., Harrison S.C., Rapoport T.A. X-ray structure of a protein-conducting channel. Nature 2004, 427:36-44.
32. Zimmer J., Nam Y., Rapoport T.A. Structure of a complex of the ATPase SecA and the protein-translocation channel. Nature 2008, 455:936-943.