Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking

Journal of Bacteriology

Volumn 181, Issue 11, 1999, Pages 3438-3444

  Harris, Chris R ^a   Silhavy, Thomas J ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN; PROTEIN SECE; SECY PROTEIN; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI; GENE MUTATION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); COLA; ESCHERICHIA; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033032483     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.11.3438-3444.1999     Document Type: Article

References (42)

1. Baba, T., T. Taura, T. Shimoike, Y. Akiyama, T. Yoshihisa, and K. Ito. 1994. A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex. Proc. Natl. Acad. Sci. USA 91:4539-4543.
2. Bardwell, J. C., J. O. Lee, G. Jander, N. Martin, D. Belin, and J. Beckwith. 1993. A pathway for disulfide bond formation in vivo. Proc. Natl. Acad. Sci. USA 90:1038-1042.
3. Bardwell, J. C. A., K. McGovern, and J. Beckwith. 1991. Identification of a protein required for disulfide bond formation in vivo. Cell 67:581-589.
4. Bieker, K. L., G. J. Phillips, and T. J. Silhavy. 1990. The sec and prl genes of Escherichia coli. J. Bioenerg. Biomembr. 22:291-310.
5. Bieker, K. L., and T. J. Silhay. 1990. PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli. Cell 61:833-842.
6. Bieker-Brady, K., and T. J. Silhavy. 1992. Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli. EMBO J. 11:3165-3174.
7. Brundage, L., J. P. Hendrick, E. Schiebel, A. J. M. Driessen, and W. Wickner. 1990. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 62:649-657.
8. Crick, F. H. C., L. Barnett, S. Brenner, and R. J. Watts-Tobin. 1961. General nature of the genetic code for proteins. Nature 192:1227-1232.
9. Deng, W. P., and J. A. Miekoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
10. Derman, A. I., W. A. Prinz, D. Belin, and J. Beckwith. 1993. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 262:1744-1747.
11. Duong, F., J. Eichler, A. Price, M. R. Leonard, and W. Wickner. 1997. Biogenesis of the Gram-negative bacterial envelope. Cell 91:567-573.
12. Economou, A. 1998. Bacterial preprotein translocase: mechanism and conformational dynamics of a processive enzyme. Mol. Microbiol. 27:511-518.
13. Economou, A., and W. Wickner. 1994. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78:835-843.
14. Esnault, Y., D. Feldheim, M.-O. Blondel, R. Schekman, and F. Kepes. 1994. SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus. J. Biol. Chem. 269:27478-27485.
15. Falke, J. J., and D. E. Koshland, Jr. 1987. Global flexibility in a sensory receptor: a site-directed cross-linking approach. Science 237:1596-1600.
16. Flower, A. M., R. S. Osborne, and T. J. Silhavy. 1995. The allele-specific lethality of prlA-prtG double mutants predicts interactive domains of SecY and SecE. EMBO J. 14:884-893.
17. Guarente, L. 1993. Synthetic enhancement in gene interaction: a genetic tool come of age. Trends Genet. 9:362-366.
18. Huffaker, T. C., M. H. Hoyt, and D. Botstein. 1987. Genetic analysis of the yeast cytoskeleton. Annu. Rev. Genet. 21:259-284.
19. Joly, J. C., M. R. Leonard, and W. Wickner. 1994. Subunit dynamics in Escherichia coli preprotein translocase. Proc. Natl. Acad. Sci. USA 91:4703-4707.
20. Kim, Y. J., T. Rajapandi, and D. Oliver. 1994. SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state. Cell 78: 845-853.
21. Kusukawa, N., T. Yura, C. Ueguchi, Y. Akiyama, and K. Ito. 1989. Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli. EMBO J. 8:3517-3521.
22. Lee, G. F., G. G. Burrows, M. R. Lebert, D. P. Dutton, and G. L. Hazelbauer. 1994. Deducing the organization of a transmembrane domain by disulfide cross-linking. The bacterial chemoreceptor Trg. J. Biol. Chem. 269:29920-29927.
23. Lingappa, V. R., J. Chaidez, C. S. Yost, and J. Hedgpeth. 1984. Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes. Proc. Natl. Acad. Sci. USA 81:456-460.
24. Mason, D. J., E. G. M. Power, H. Talsania, I. Phillips, and V. A. Gant. 1995. Antimicrobial action of ciprofloxacin. Antimicrob. Agents Chemother. 39: 2752-2758.
25. Meyer, T. H., J. F. Menetret, R. Breitling, K. R. Miller, C. W. Akey, and T. A. Rapoport. 1999. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J. Mol. Biol. 285:1789-1800.
26. Milligan, D. L., and D. E. Koshland, Jr. 1992. Site-directed cross-linking: establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis. J. Biol. Chem. 263:6268-6275.
27. Nishiyama, K., T. Suzuki, and H. Tokuda. 1996. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85:71-81.
28. Osborne, R. S., and T. J. Silhavy. Unpublished data.
29. Osborne, R. S, and T. J. Silhavy. 1993. PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains. EMBO J. 12: 3391-3398.
30. Pakula, A. A., and M. I. Simon. 1992. Determination of transmembrane protein structure by disulfide cross-linking: the Escherichia coli Tar receptor. Proc. Natl. Acad. Sci. USA 89:4144-4148.
31. Phillips, G. J., and T. J. Silhavy. 1992. The E. coli ffh gene is necessary for viability and efficient protein export. Nature 359:744-746.
32. Pogliano, K. J., and J. Beckwith. 1993. The cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself. Genetics 133:763-773.
33. Pohlschrnder, M., C. Murphy, and J. Beckwith. 1996. In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery. J. Biol. Chem. 271:19908-19914.
34. Pohlschroder, M., W. A. Prinz, E. Hartmann, and J. Beckwith. 1997. Protein translocation in the three domains of life: variations on a theme. Cell 91: 563-566.
35. Rapoport, T. A., B. Jungnickel, and U. Kutay. 1996. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu. Rev. Biochem. 65:271-303.
36. Roth, B. L., M. Pool, S. T. Yue, and P. J. Millard. 1997. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl. Environ. Microbiol. 63:2421-2431.
37. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
38. Talmadge, K., S. Stahl, and W. Gilbert. 1980. Bacteria mature preproinsulin to proinsulin. Proc. Natl. Acad. Sci. USA 77:3369-3373.
39. Ulbrandt, N. D., J. A. Jewitt, and H. D. Bernstein. 1997. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins. Cell 88:187-196.
40. von Heijne, G. 1985. Signal sequences: the limits of variation. J. Mol. Biol. 184:99-105.
41. Whitley, P., L. Nilsson, and G. von Heijne. 1993. Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry 32:8534-8539.
42. Wilkinson, B. M., Y. Esnault, R. A. Craven, F. Skiba, J. Fieschi, F. Kepes, and C. J. Stirling. 1997. Molecular architecture of the ER translocase probed by chemical crosslinking of Sss1p to complementary fragments of Sec61p. EMBO J. 16:4549-4559.