Atomic model of the E. coli membrane-bound protein translocation complex SecYEG

Journal of Molecular Biology

Volumn 352, Issue 5, 2005, Pages 1035-1043

  Bostina, Mihnea ^a   Mohsin, Brigitte ^a   Kühlbrandt, Werner ^a   Collinson, Ian ^a,b  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Conformational changes; Membrane bound; Model; Protein translocation; Structure

Indexed keywords

PROTEIN; PROTEIN SECYEG; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC PARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIMERIZATION; ESCHERICHIA COLI; GENE TRANSLOCATION; METHANOCOCCUS JANNASCHII; MOLECULAR MODEL; NONHUMAN; PREDICTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII;

EID: 24944458963     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.08.005     Document Type: Article

References (35)

1. C. Milstein, G.G. Brownlee, T.M. Harrison, and M.B. Mathews A possible precursor of immunoglobulin light chains Nature New Biol. 239 1972 117 120
2. G. Blobel, and B. Dobberstein Trasnsfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma J. Cell Biol. 67 1975 835 851
3. T.A. Rapoport, V. Goder, S.U. Heinrich, and K.E. Matlack Membrane-protein integration and the role of the translocation channel Trends Cell Biol. 14 2004 568 575
4. K.E. Matlack, W. Mothes, and T.A. Rapoport Protein translocation: tunnel vision Cell 92 1998 381 390
5. M. Pohlschroder, W. Prinz, E. Hartmann, and J. Beckwith Protein translocation in the three domains of life: variations on a theme Cell 91 1997 563 566
6. L. Brundage, J.P. Hendrick, E. Schiebel, A.J.M. Driessen, and W. Wickner The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation Cell 62 1990 649 657
7. F. Duong, and W. Wickner Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer function EMBO J. 17 1998 696 705
8. D. Oliver, and J. Beckwith Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli J. Bacteriol. 150 1982 686 691
9. R.A. Arkowitz, J.C. Joly, and W. Wickner Translocation can drive the unfolding of a preprotein domain EMBO J. 12 1993 243 253
10. D. Görlich, S. Prehn, E. Hartmann, K. Kalies, and T. Rapoport A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation Cell 71 1992 489 503
11. W. Mothes, S. Heinrich, R. Graf, I. Nilsson, G. von Heijne, J. Brunner, and T. Rapoport Molecular mechanism of membrane protein integration into the endoplasmic reticulum Cell 89 1997 523 533
12. K. Beck, L. Wu, J. Brunner, and M. Muller Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor EMBO J. 19 2000 134 143
13. D. Görlich, and T.A. Rapoport Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane Cell 75 1993 615 630
14. S.U. Heinrich, W. Mothes, J. Brunner, and T.A. Rapoport The Sec61p complex mediates the integration of a membrane protein by allowing lipid partitioning of the transmembrane domain Cell 102 2000 233 244
15. C. Breyton, W. Haase, T.A. Rapoport, W. Kühlbrandt, and I. Collinson Three-dimensional structure of the bacterial protein-translocation complex SecYEG Nature 418 2002 662 665
16. L. van den Berg, W.M.J. Clemons, I. Collinson, Y. Modis, E. Hartmann, S.C. Harrison, and T.A. Rapoport X-ray structure of a protein-conducting channel Nature 427 2004 36 44
17. C. Tziatzios, D. Schubert, M. Lotz, D. Gundogan, H. Betz, and H. Schägger The bacterial protein-translocation complex: dimeric SecYEG associates with both one or two molecules of SecA J. Mol. Biol. 340 2004 513 524
18. P. Bessonneau, V. Besson, I. Collinson, and F. Duong The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure EMBO J. 21 2002 995 1003
19. E.H. Manting, C. van der Does, H. Remigy, A. Engel, and A.J. Driessen SecYEG assembles into a tetramer to form the active protein translocation channel EMBO J. 19 2000 852 861
20. R. Beckmann, C. Spahn, N. Eswar, J. Helmers, P. Penczek, and A. Sali Architecture of the protein-conducting channel associated with the translating 80 S ribosome Cell 107 2001 361 372
21. F. Duong Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase EMBO J. 22 2003 4375 4384
22. D. Hanein, K. Matlack, B. Jungnickel, K. Plath, K. Kalies, and K. Miller Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation Cell 87 1996 721 732
23. J.F. Menetret, R.S. Hegde, S.U. Heinrich, P. Chandramouli, S.J. Ludtke, T.A. Rapoport, and C.W. Akey Architecture of the ribosome-channel complex derived from native membranes J. Mol. Biol. 348 2005 445 457
24. I. Collinson, C. Breyton, F. Duong, C. Tziatzios, D. Schubert, and E. Or Projection structure and oligomeric properties of a bacterial core protein translocase EMBO J. 20 2001 2462 2471
25. C. Chothia, and A.M. Lesk The relation between the divergence of sequence and structure in proteins EMBO J. 5 1986 823 826
26. H. Mori, and K. Ito An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY Proc. Natl Acad. Sci. USA 98 2001 5128 5133
27. G. Matsumoto, T. Yoshihisa, and K. Ito SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane EMBO J. 16 1997 6384 6393
28. K. Plath, W. Mothes, B. Wilkinson, C. Stirling, and T. Rapoport Signal sequence recognition in posttranslational protein transport across the yeast ER membrane Cell 94 1998 795 807
29. W. Wriggers, R.A. Milligan, and J.A. McCammon Situs: A package for docking crystal structures into low-resolution maps from electron microscopy J. Struct. Biol. 125 1999 185 195
30. +-ATPase Science 297 2002 1692 1696
31. A. Veenendaal, C. van der Does, and A. Driessen Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis J. Biol. Chem. 276 2001 32559 32566
32. A. Flower, R. Osborne, and T. Silhavy The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE EMBO J. 14 1995 884 893
33. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucl. Acids Res. 22 1994 4673 4680
34. A. Sali, and T.L. Blundell Comparative protein modelling by satisfaction of spatial restraints J. Mol. Biol. 234 1993 779 815
35. P.J. Kraulis MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures J. Appl. Crystallog. 24 1991 946 950