Three-dimensional structure of the bacterial protein-translocation complex SecYEG

Nature

Volumn 418, Issue 6898, 2002, Pages 662-665

  Breyton, Cécile ^a,c   Haase, Winfried ^a   Rapoport, Tom A ^b   Kühlbrandt, Werner ^a   Collinson, Ian ^a,b  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

^c CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; ESCHERICHIA COLI; POLYPEPTIDES; STRUCTURE (COMPOSITION);

TRANSMEMBRANE HELICES;

PROTEINS;

BACTERIAL PROTEIN; DIMER; PROTEIN SUBUNIT;

BIOLOGY; PROTEIN;

ARTICLE; BACTERIAL MEMBRANE; COMPLEX FORMATION; ESCHERICHIA COLI; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; THREE DIMENSIONAL IMAGING;

CELL MEMBRANE; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; DIMERIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037043724     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature00827     Document Type: Article

References (30)

1. Protein translocation: Tunnel vision
2. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation
3. Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli
4. A mammalian homolog of SEC 61p and SECYp is associated with ribosomes and nascent polypeptides during translocation
5. Oligomeric ring of the Sec 61p complex induced by ligands required for protein translocation
6. The structure of ribosome-channel complexes engaged in protein translocation
7. Architecture of the protein-conducting channel associated with the translocating 80S ribosome
8. SecYEG assembles into a tetramer to form the active protein translocation channel
9. Projection structure and oligomeric properties of a bacterial core protein translocase
10. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
11. Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli
12. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme
13. Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature
14. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure
15. Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighbouring SecE
16. The allele-specific synthetic lethality of pr1A-pr 1G double mutants predicts interactive domains of SecY and SecE
17. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane
18. Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis
19. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
20. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events
21. The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase
22. Escherichia coli SecA shape and dimensions
23. Use of antibody fragments (Fv) in immunocytochemistry
24. SDS-digested freeze-fracture replica labeling electron microscopy to study the two-dimensional distribution of integral membrane proteins and phospholipids in biomembranes: Practical procedure, interpretation and application
25. Structure of purple membrane from Halobacterium halobium. Recording, measurement and evaluation of electron micrographs at 3.5 Ä resolution
26. MRC image processing programs
27. Electron-crystallographic refinement of the structure of bacteriorhodopsin
28. Assessment of electron crystallographic data obtained from two-dimensional crystals of biological specimens
29. The CCP4 suite: Programs for protein crystallography
30. Improved methods for building protein models in electron density maps