Micro segmented-flow in biochemical and cell-based assays

Frontiers in Bioscience - Elite

Volumn 4 E, Issue 5, 2012, Pages 1768-1779

  Clausell Tormos, Jenifer ^a   Merten, Christoph A ^b  

^a Structural Biology and Biocomputing Programme   (Spain)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Biochemical and cell based screens; Droplets; Emulsions; Microfluidics; Plugs; Review; Segmented flow; Single cell assays

Indexed keywords

MEMBRANE PROTEIN; ENZYME;

CAPILLARY ELECTROPHORESIS; CELL ENCAPSULATION; CELL LYSATE; CRYSTALLIZATION; EMULSION; ENZYME KINETICS; GENETIC ALGORITHM; MICROFLUIDICS; MOLECULAR BIOLOGY; REVIEW; TRANSCRIPTOMICS; ALGORITHM; CATALYSIS; KINETICS; METABOLISM; METHODOLOGY;

ALGORITHMS; CATALYSIS; CRYSTALLIZATION; ELECTROPHORESIS, CAPILLARY; ENZYMES; KINETICS; MICROFLUIDICS;

EID: 84860877548     PISSN: 19450494     EISSN: 19450508     Source Type: Journal    
DOI: None     Document Type: Review

References (46)

1. Desbordes, S. C., D. G. Placantonakis, A. Ciro, N. D. Socci, G. Lee, H. Djaballah & L. Studer: High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell, 2, 602-12 (2008) (Pubitemid 351729221)
2. Shum, D., C. Radu, E. Kim, M. Cajuste, Y. Shao, V. E. Seshan & H. Djaballah: A high density assay format for the detection of novel cytotoxic agents in large chemical libraries. J Enzyme Inhib Med Chem, 23, 931-45 (2008)
3. Brandish, P. E., C. S. Chiu, J. Schneeweis, N. J. Brandon, C. L. Leech, O. Kornienko, E. M. Scolnick, B. Strulovici & W. Zheng: A cell-based ultra-high-throughput screening assay for identifying inhibitors of D-amino acid oxidase. J Biomol Screen, 11, 481-7 (2006) (Pubitemid 44222999)
4. Oldenburg, K. R., J.-H. Zhang, T. Chen, A. Maffia, K. F. Blom, A. P. Combs & T. D. Y. Chung: Assay Miniaturization for Ultra-High Throughput Screening of Combinatorial and Discrete Compound Libraries: A 9600-Well (0.2 Microliter) Assay System. Journal of Biomolecular Screening, 3, 55-62 (1998) (Pubitemid 128659555)
5. Berg, M., K. Undisz, R. Thiericke, P. Zimmermann, T. Moore & C. Posten: Evaluation of liquid handling conditions in microplates. J Biomol Screen, 6, 47-56 (2001) (Pubitemid 32172444)
6. Dove, A.: Drug screening - beyond the bottleneck. Nat Biotechnol, 17, 859-63 (1999) (Pubitemid 29416657)
7. Vyawahare, S., A. D. Griffiths & C. A. Merten: Miniaturization and parallelization of biological and chemical assays in microfluidic devices. Chem Biol, 17, 1052-65 (2010)
8. Stone, H. D. & Z. X. Xie: Efficacy of experimental Newcastle disease water-in-oil oil-emulsion vaccines formulated from squalane and squalene. Avian Dis, 34, 979-83 (1990)
9. Izquierdo, P., J. Feng, J. Esquena, T. F. Tadros, J. C. Dederen, M. J. Garcia, N. Azemar & C. Solans: The influence of surfactant mixing ratio on nano-emulsion formation by the pit method. J Colloid Interface Sci, 285, 388-94 (2005) (Pubitemid 41350451)
10. Bai, Y., X. He, D. Liu, S. N. Patil, D. Bratton, A. Huebner, F. Hollfelder, C. Abell & W. T. Huck: A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip, 10, 1281-5 (2010)
11. Courtois, F., L. F. Olguin, G. Whyte, A. B. Theberge, W. T. Huck, F. Hollfelder & C. Abell: Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Anal Chem, 81, 3008-16 (2009)
12. Zheng, B. & R. F. Ismagilov: A microfluidic approach for screening submicroliter volumes against multiple reagents by using preformed arrays of nanoliter plugs in a three-phase liquid/liquid/gas flow. Angew Chem Int Ed Engl, 44, 2520-3 (2005) (Pubitemid 40600319)
13. Baret, J. C., V. Taly, M. Ryckelynck, C. A. Merten & A. D. Griffiths: [Droplets and emulsions: very highthroughput screening in biology]. Med Sci (Paris), 25, 627-32 (2009)
14. Kelly, B. T., J. C. Baret, V. Taly & A. D. Griffiths: Miniaturizing chemistry and biology in microdroplets. Chemical Communications1773-1788 (2007) (Pubitemid 46699184)
15. Theberge, A. B., F. Courtois, Y. Schaerli, M. Fischlechner, C. Abell, F. Hollfelder & W. T. S. Huck: Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology. Angewandte Chemie-International Edition, 49, 5846-5868 (2010)
16. Song, H., D. L. Chen & R. F. Ismagilov: Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl, 45, 7336-56 (2006) (Pubitemid 44786956)
17. Teh, S. Y., R. Lin, L. H. Hung & A. P. Lee: Droplet microfluidics. Lab Chip, 8, 198-220 (2008) (Pubitemid 351191417)
18. Chen, D. L. & R. F. Ismagilov: Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening. Curr Opin Chem Biol, 10, 226-31 (2006) (Pubitemid 43815780)
19. Chen, D. L., L. Li, S. Reyes, D. N. Adamson & R. F. Ismagilov: Using three-phase flow of immiscible liquids to prevent coalescence of droplets in microfluidic channels: criteria to identify the third liquid and validation with protein crystallization. Langmuir, 23, 2255-60 (2007) (Pubitemid 46406190)
20. Chen, D., W. Du, Y. Liu, W. Liu, A. Kuznetsov, F. E. Mendez, L. H. Philipson & R. F. Ismagilov: The chemistrode: a droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution. Proc Natl Acad Sci U S A, 105, 16843-8 (2008)
21. Chabert, M., K. D. Dorfman, P. de Cremoux, J. Roeraade & J. L. Viovy: Automated microdroplet platform for sample manipulation and polymerase chain reaction. Anal Chem, 78, 7722-8 (2006)
22. Clausell-Tormos, J., A. D. Griffiths & C. A. Merten: An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries. Lab Chip, 10, 1302-7 (2010)
23. Fang, X., Y. Liu, J. Kong & X. Jiang: Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens. Anal Chem, 82, 3002-6 (2010)
24. Martin, K., T. Henkel, V. Baier, A. Grodrian, T. Schön, M. Roth, J. Köhler & J. Metze: Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices. Lab on a Chip, 3, 202-207 (2003) (Pubitemid 41646556)
25. Srisa-Art, M., D. K. Kang, J. Hong, H. Park, R. J. Leatherbarrow, J. B. Edel, S. I. Chang & A. J. deMello: Analysis of Protein-Protein Interactions by Using Droplet-Based Microfluidics. Chembiochem, 10, 1605-1611 (2009)
26. Robinson, T., Y. Schaerli, R. Wootton, F. Hollfelder, C. Dunsby, G. Baldwin, M. Neil, P. French & A. deMello: Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip, 9, 3437-41 (2009)
27. Cecchini, M. P., J. Hong, C. Lim, J. Choo, T. Albrecht, A. J. Demello & J. B. Edel: Ultrafast Surface Enhanced Resonance Raman Scattering Detection in Droplet-Based Microfluidic Systems. Anal Chem (2011)
28. Fidalgo, L. M., G. Whyte, B. T. Ruotolo, J. L. Benesch, F. Stengel, C. Abell, C. V. Robinson & W. T. Huck: Coupling microdroplet microreactors with mass spectrometry: reading the contents of single droplets online. Angew Chem Int Ed Engl, 48, 3665-8 (2009)
29. Kautz, R. A., W. K. Goetzinger & B. L. Karger: High-throughput microcoil NMR of compound libraries using zero-dispersion segmented flow analysis. J Comb Chem, 7, 14-20 (2005) (Pubitemid 40221561)
30. Rane, T. D., C. M. Puleo, K. J. Liu, Y. Zhang, A. P. Lee & T. H. Wang: Counting single molecules in subnanolitre droplets. Lab Chip, 10, 161-4 (2010)
31. Li, L., D. Mustafi, Q. Fu, V. Tereshko, D. L. Chen, J. D. Tice & R. F. Ismagilov: Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins. Proc Natl Acad Sci U S A, 103, 19243-8 (2006)
32. Gerdts, C. J., V. Tereshko, M. K. Yadav, I. Dementieva, F. Collart, A. Joachimiak, R. C. Stevens, P. Kuhn, A. Kossiakoff & R. F. Ismagilov: Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization. Angew Chem Int Ed Engl, 45, 8156-60 (2006) (Pubitemid 44974151)
33. Senkan, S.: Combinatorial Heterogeneous Catalysis - A New Path in an Old Field. Angewandte Chemie International Edition, 40, 312-329 (2001) (Pubitemid 32114870)
34. Vriamont, N., B. Govaerts, P. Grenouillet, C. de Bellefon & O. Riant: Design of a Genetic Algorithm for the Simulated Evolution of a Library of Asymmetric Transfer Hydrogenation Catalysts. Chemistry - A European Journal, 15, 6267-6278 (2009)
35. Kreutz, J. E., A. Shukhaev, W. Du, S. Druskin, O. Daugulis & R. F. Ismagilov: Evolution of catalysts directed by genetic algorithms in a plug-based microfluidic device tested with oxidation of methane by oxygen. J Am Chem Soc, 132, 3128-32
36. Song, H. & R. F. Ismagilov: Millisecond kinetics on a microfluidic chip using nanoliters of reagents. J Am Chem Soc, 125, 14613-9 (2003)
37. Niu, X. Z., B. Zhang, R. T. Marszalek, O. Ces, J. B. Edel, D. R. Klug & A. J. deMello: Droplet-based compartmentalization of chemically separated components in two-dimensional separations. Chem Commun (Camb)6159-61 (2009)
38. Martin, K., T. Henkel, V. Baier, A. Grodrian, T. Schon, M. Roth, J. Michael Kohler & J. Metze: Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices. Lab Chip, 3, 202-7 (2003) (Pubitemid 41646556)
39. Ventura, M., F. Turroni, C. Canchaya, E. E. Vaughan, P. W. O'Toole & D. van Sinderen: Microbial diversity in the human intestine and novel insights from metagenomics. Front Biosci, 14, 3214-21 (2009)
40. Singh, J., A. Behal, N. Singla, A. Joshi, N. Birbian, S. Singh, V. Bali & N. Batra: Metagenomics: Concept, methodology, ecological inference and recent advances. Biotechnol J, 4, 480-94 (2009)
41. Grodrian, A., J. Metze, T. Henkel, K. Martin, M. Roth & J. M. Kohler: Segmented flow generation by chip reactors for highly parallelized cell cultivation. Biosens Bioelectron, 19, 1421-8 (2004)
42. He, M., J. S. Edgar, G. D. Jeffries, R. M. Lorenz, J. P. Shelby & D. T. Chiu: Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Anal Chem, 77, 1539-44 (2005) (Pubitemid 40396244)
43. Funfak, A., A. Brosing, M. Brand & J. M. Kohler: Micro fluid segment technique for screening and development studies on Danio rerio embryos. Lab Chip, 7, 1132-8 (2007)
44. Clausell-Tormos, J., D. Lieber, J. C. Baret, A. El-Harrak, O. J. Miller, L. Frenz, J. Blouwolff, K. J. Humphry, S. Koster, H. Duan, C. Holtze, D. A. Weitz, A. D. Griffiths & C. A. Merten: Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms. Chem Biol, 15, 427-37 (2008) (Pubitemid 351645111)
45. Schena, M., D. Shalon, R. W. Davis & P. O. Brown: Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science, 270, 467-70 (1995)
46. Bontoux, N., L. Dauphinot, T. Vitalis, V. Studer, Y. Chen, J. Rossier & M. C. Potier: Integrating whole transcriptome assays on a lab-on-a-chip for single cell gene profiling. Lab Chip, 8, 443-50 (2008) (Pubitemid 351322624)