A cell-based ultra-high-throughput screening assay for identifying inhibitors of D-amino acid oxidase

Journal of Biomolecular Screening

Volumn 11, Issue 5, 2006, Pages 481-487

  Brandish, Philip E ^a   Chiu, Chi Sung ^a   Schneeweis, Jonathan ^a   Brandon, Nicholas J ^b   Leech, Clare L ^b   Kornienko, Oleg ^a   Scolnick, Edward M ^a,d   Strulovici, Berta ^a   Zheng, Wei ^a,c,e  

^a Department of Neurobiology ^*  (United States)

^b Merck Sharp & Dohme Limited   (United Kingdom)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

^d BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^e NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

Author keywords

Cell based enzyme assay; D amino acid oxidase; DAO; High throughput screening; Schizophrenia; uHTS

Indexed keywords

DEXTRO AMINO ACID OXIDASE; DEXTRO SERINE; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; INOSITOL PHOSPHATE;

ANIMAL CELL; ARTICLE; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; ENZYME ACTIVITY; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCHIZOPHRENIA; SIGNAL NOISE RATIO;

ANIMALS; AUTOMATION; CHO CELLS; CRICETINAE; D-AMINO-ACID OXIDASE; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; HUMANS; MODELS, BIOLOGICAL; TISSUE ARRAY ANALYSIS; TRANSFECTION;

CRICETULUS GRISEUS;

EID: 33747102410     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057106288181     Document Type: Article

References (20)

1. Bromet EJ, Fennig S: Epidemiology and natural history of schizophrenia. Biol Psychiatry 1999;46:871-881.
2. Javitt DC: Treatment of negative and cognitive symptoms. Curr Psychiatry Rep 1999;1(1):25-30.
3. Adams B, Moghaddam B: Corticolimbic dopamine neurotransmission is temporally dissociated from the cognitive and locomotor effects of phencyclidine. J Neurosci 1998;18:5545-5554.
4. Schell MJ: The N-methyl D-aspartate receptor glycine site and D-serine metabolism: an evolutionary perspective. Philos Trans R Soc Lond B Biol Sci 2004;359:943-964.
5. Miyamoto S, Duncan GE, Marx CE, Lieberman JA: Treatments for schizophrenia: a critical review of pharmacology and mechanisms of action of antipsychotic drugs. Mol Psychiatry 2004;10(1):79-104.
6. Goff DC, Henderson DC, Evins AE, Amico E: A placebo-controlled crossover trial of D-cycloserine added to clozapine in patients with schizophrenia. Biol Psychiatry 1999;45:512-514.
7. Heresco-Levy U, Javitt DC, Ermilov M, Mordel C, Silipo G, Lichtenstein M: Efficacy of high-dose glycine in the treatment of enduring negative symptoms of schizophrenia. Arch Gen Psychiatry 1999;56(1):29-36.
8. Tsai GE, Yang P, Chung LC, Tsai IC, Tsai CW, Coyle JT: D-serine added to clozapine for the treatment of schizophrenia. Am J Psychiatry 1999;156:1822-1825.
9. Chumakov I, Blumenfeld M, Guerassimenko O, et al: Genetic and physiological data implicating the new human gene G72 and the gene for D-amino acid oxidase in schizophrenia. Proc Natl Acad Sci USA 2002;99:13675-13680.
10. Raibekas AA, Fukui K, Massey V: Design and properties of human D-amino acid oxidase with covalently attached flavin. Proc Natl Acad Sci USA 2000;97:3089-3093.
11. Fukui K, Miyake Y: Molecular cloning and chromosomal localization of a human gene encoding D-amino-acid oxidase. J Biol Chem 1992;267:18631-18638.
12. Raibekas AA, Fukui K, Massey V: Design and properties of human D-amino acid oxidase with covalently attached flavin. Proc Natl Acad Sci USA 2000;97:3089-3093.
13. Curti B, Ronchi S, Simonetta, MP: The D- and L-amino acid oxidase. In: (Muller, F., ed.) Chemistry and Biochemistry of Flavoenzymes. Boca Raton, FL: CRC Press; 1992;3:69-94.
14. Kornienko O, Lacson R, Kunapuli P, Schneeweis J, Hoffman I, Smith T, et al: Miniaturization of whole live cell-based GPCR assays using microdispensing and detection systems. J Biomol Screen 2004;9:186-195.
15. Brandish PE, Hill LA, Zheng W, Scolnick EM: Scintillation proximity assay of inositol phosphates in cell extracts: high-throughput measurement of G-protein-coupled receptor activation. Anal Biochem 2003;313:311-318.
16. Zheng W, Brandish PE, Kolodin DG, Scolnick EM, Strulovici B: High-throughput cell-based screening using scintillation proximity assay for the discovery of inositol phosphatase inhibitors. J Biomol Screen 2004;9:132-140.
17. Biondi PA, Guidotti L, Negri A, Secchi C: High-performance liquid chromatographic determination of D-amino acid oxidase activity. J Chromatogr 1991;566:377-382.
18. Carone FA, Nakamura S, Goldman B: Urinary loss of glucose, phosphate, and protein by diffusion into proximal straight tubules injured by D-serine and maleic acid. Lab Invest 1985;52:605-610.
19. Stahura FL, Bajorath J: Virtual screening methods that complement HTS. Comb Chem High Throughput Screen 2004;7:259-269.
20. Kitchen DB, Stahura FL, Bajorath J: Computational techniques for diversity analysis and compound classification. Mini Rev Med Chem 2004;4:1029-1039.