Developing an extended genomic engineering approach based on recombineering to knock-in heterologous genes to Escherichia coli genome

Molecular Biotechnology

Volumn 51, Issue 2, 2012, Pages 109-118

  Sukhija, Karan ^a   Pyne, Michael ^a   Ali, Saad ^a   Orr, Valerie ^a   Abedi, Daryoush ^b   Moo Young, Murray ^a   Chou, C Perry ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b ISFAHAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Escherichia coli; Genomic engineering; Lipase; Penicillin acylase; Recombinant protein; Recombineering

Indexed keywords

ANTARCTICA; BIOMANUFACTURING; BIOTECHNOLOGICAL APPLICATIONS; E. COLI; FOREIGN GENES; GENE KNOCKOUT; GENE REPLACEMENT; GENETIC BACKGROUNDS; HETEROLOGOUS GENES; KANAMYCINS; PENICILLIN ACYLASE; RECOMBINEERING; REGULATORY ELEMENTS; SELECTION MARKER;

ANTIBIOTICS; CHROMOSOMES; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; LIPASES; RECOMBINANT PROTEINS;

GENE EXPRESSION REGULATION;

ISOPROPYL THIOGALACTOSIDE;

ARTICLE; BACTERIAL GENETICS; BACTERIOPHAGE; BINDING SITE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION; GENE INSERTION; GENE REPLACEMENT THERAPY; GENE TARGETING; GENETIC ENGINEERING; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; NONHUMAN; OPERON;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE KNOCK-IN TECHNIQUES; GENETIC ENGINEERING; GENOME, BACTERIAL; LIPASE; MODELS, GENETIC; PENICILLIN AMIDASE; RECOMBINANT PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID;

ENTEROBACTERIA PHAGE LAMBDA; ESCHERICHIA COLI; PSEUDOZYMA ANTARCTICA;

EID: 84860840542     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9442-2     Document Type: Article

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