Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: The FLIRT system

Journal of Bacteriology

Volumn 179, Issue 19, 1997, Pages 6076-6083

  Huang, Li Chun ^a   Wood, Elizabeth A ^a   Cox, Michael M ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RECOMBINASE;

ARTICLE; CHROMOSOME; CONTROLLED STUDY; ELECTROPORATION; ESCHERICHIA COLI; GENETIC RECOMBINATION; GENOME; NONHUMAN; PRIORITY JOURNAL; TRANSCRIPTION INITIATION; TRANSPOSON; YEAST;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030883633     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.19.6076-6083.1997     Document Type: Article

References (53)

1. Ayres, E. K., V. J. Thomson, G. Merino, D. Balderes, and D. H. Figurski. 1993. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230:174-185.
2. Baubonis, W., and B. Sauer. 1993. Genomic targeting with purified Cre recombinase. Nucleic Acids Res. 21:2025-2029.
3. Bayley, C. C., M. Morgan, E. C. Dale, and D. W. Ow. 1992. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Mol. Biol. 18:353-361.
4. Bernet, A., S. Sabatier, D. J. Picketts, R. Ouazana, F. Morle, D. R. Higgs, and J. Godet. 1995. Targeted inactivation of the major positive regulatory element (HS-40) of the human alpha-globin gene locus. Blood 86:1202-1211.
5. Cherepanov, P. P., and W. Wackernagel. 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158:9-14.
6. Cox, M. M. 1983. The FLP protein of the yeast 2-micron plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli. Proc. Natl. Acad. Sci. USA 80:4223-4227.
7. Cox, M. M. 1989. DNA inversion in the 2μm plasmid of Saccharomyces cerevisiae, p. 661-670. In D. E. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D.C.
8. Craig, N. L. 1988. The mechanism of conservative site-specific recombination. Annu. Rev. Genet. 22:77-105.
9. Daum, H., III. Personal communication.
10. Davis, R. W., D. Botstein, and J. R. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
11. DiSanto, J. P., W. Muller, G. D. Guy, A. Fischer, and K. Rajewsky. 1995. Lymphoid development in mice with a targeted deletion of the interleukin 2 receptor gamma chain. Proc. Natl. Acad. Sci. USA 92:377-381.
12. Fiering, S., E. Epner, K. Robinson, Y. Zhuang, A. Telling, M. Hu, D. I. Martin, T. Enver, T. J. Ley, and M. Groudine. 1995. Targeted deletion of 5′HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes Dev. 9:2203-2213.
13. Fiering, S., C. G. Kim, E. M. Epner, and M. Groudine. 1993. An "in-out" strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: analysis of the beta-globin locus control region. Proc. Natl. Acad. Sci. USA 90:8469-8473.
14. Fukushige, S., and B. Sauer. 1992. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 89:7905-7909.
15. Fuse, T., H. Kodama, N. Hayashida, K. Shinozaki, M. Nishimura, and K. Iba. 1995. A novel Ti-plasmid-convertible lambda phage vector system suitable for gene isolation by genetic complementation of Arabidopsis thaliana mutants. Plant J. 7:849-856.
16. Gage, P. J., B. Sauer, M. Levine, and J. C. Glorioso. 1992. A cell-free recombination system for site-specific integration of multigenic shuttle plasmids into the herpes simplex virus type 1 genome. J. Virol. 66:5509-5515.
17. Golic, K. G. 1994. Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase. Genetics 137:551-563.
18. Golic, K. G., and S. Lindquist. 1989. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59:499-509.
19. Gu, H., Y. R. Zou, and K. Rajewsky. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 73:1155-1164.
20. Guyer, M. S., R. R. Reed, J. A. Steitz, and K. B. Low. 1981. Identification of a sex factor affinity site in E. coli as γσδ. Cold Spring Harbor Symp. Quant. Biol. 45:135-140.
21. Huang, L. C. 1992. A bacterial model system for chromosomal targeting. Ph.D. thesis. University of Wisconsin - Madison, Madison.
22. Huang, L. C., E. A. Wood, and M. M. Cox. 1991. A bacterial model system for chromosomal targeting. Nucleic Acids Res. 19:443-448.
23. Jayaram, M. 1994. Phosphoryl transfer in Flp recombination: a template for strand transfer mechanisms. Trends Biochem. Sci. 19:78-82.
24. Johnson, R. C., J. C. Yin, and W. S. Reznikoff. 1982. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat. Cell 30:873-882.
25. Lakso, M., B. Sauer, B. J. Mosinger, E. J. Lee, R. W. Manning, S. H. Yu, K. L. Mulder, and H. Westphal. 1992. Targeted oncogene activation by site-specific recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 89:6232-6236.
26. Lakso, M., J. G. Pichel, J. R. Gorman, B. Sauer, Y. Okamoto, E. Lee, F. W. Alt, and H. Westphal. 1996. Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc. Natl. Acad. Sci. USA 93:5860-5865.
27. Leslie, N. R., and D. J. Sherratt. 1995. Site-specific recombination in the replication terminus region of Escherichia coli: functional replacement of dif. EMBO J. 14:1561-1570.
28. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
29. Medberry, S. L., E. Dale, M. Qin, and D. W. Ow. 1995. Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination. Nucleic Acids Res. 23:485-490.
30. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
31. Morris, A. C., T. L. Schaub, and A. A. James. 1991. FLP-mediated recombination in the vector mosquito, Aedes aegypti. Nucleic Acids Res. 19:5895-5900.
32. O'Gorman, S., D. T. Fox, and G. M. Wahl. 1991. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251:1351-1355.
33. Osborne, B. I., U. Wirtz, and B. Baker. 1995. A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. Plant J. 7:687-701.
34. Posfai, G., M. Koob, Z. Hradecna, N. Hasan, M. Filutowicz, and W. Szybalski. 1994. In vivo excision and amplification of large segments of the Escherichia coli genome. Nucleic Acids Res. 22:2392-2398.
35. Qin, M., E. Lee, T. Zankel, and D. W. Ow. 1995. Site-specific cleavage of chromosomes in vitro through Cre-lox recombination. Nucleic Acids Res. 23:1923-1927.
36. Ramirez, S. R., P. Liu, and A. Bradley. 1995. Chromosome engineering in mice. Nature 378:720-724.
37. Reznikoff, W. Unpublished data.
38. Sadowski, P. D. 1995. The Flp recombinase of the 2-micron plasmid of Saccharomyces cerevisiae. Prog. Nucleic Acid Res. Mol. Biol. 51:53-91.
39. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
40. Sauer, B. 1992. Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance. J. Mol. Biol. 223:911-928.
41. Sauer, B. 1993. Manipulation of transgenes by site-specific recombination: use of Cre recombinase. Methods Enzymol. 225:890-900.
42. Sauer, B. 1994. Recycling selectable markers in yeast. BioTechniques 16: 1086-1088.
43. Sauer, B. 1994. Site-specific recombination: developments and applications. Curr. Opin. Biotechnol. 5:521-527.
44. Senecoff, J. F., R. C. Bruckner, and M. M. Cox. 1985. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site. Proc. Natl. Acad. Sci. USA 82:7270-7274.
45. Slater, S., and R. Maurer. 1993. Simple phagemid-based system for generating allele replacements in Escherichia coli. J. Bacteriol. 175:4260-4262.
46. Smith, A. J., S. M. De, A. B. Kwabi, P. A. Heppell, H. Impey, and P. Rabbitts. 1995. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nat. Genet. 9:376-385.
47. Van, D. J., M. Fornerod, R. B. Van, and G. Grosveld. 1995. Cre-mediated site-specific translocation between nonhomologous mouse chromosomes. Proc. Natl. Acad. Sci. USA 92:7376-7380.
48. Waite, L. L., and M. M. Cox. 1995. A protein dissociation step limits turnover in FLP recombinase-mediated site-specific recombination. J. Biol. Chem. 270:23409-23414.
49. Wang, P., M. Anton, F. L. Graham, and S. Bacchetti. 1995. High frequency recombination between loxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase. Somatic Cell Mol. Genet. 21:429-441.
50. Weinreich, M. D., H. Yigit, and W. S. Reznikoff. 1994. Overexpression of the Tn5 transposase in Escherichia coli results in filamentation, aberrant nucleoid segregation, and cell death: analysis of E. coli and transposase suppressor mutations. J. Bacteriol. 176:5494-5504.
51. Wu, H. Y., S. H. Shyy, J. C. Wang, and L. F. Liu. 1988. Transcription generates positively and negatively supercoiled domains in the template. Cell 53:433-440.
52. Xu, T., and G. M. Rubin. 1993. Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117:1223-1237.
53. Yu, G.-L., and E. H. Blackburn. 1990. Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila. Mol. Cell. Biol. 10:2070-2080.