Deletion mutant comprising 198 residues of BoNT/A toxin receptor binding domain retained GT1b binding property but failed to induce protective antibody response in a mouse model

Protein and Peptide Letters

Volumn 19, Issue 5, 2012, Pages 530-537

  Kumar Singh, Manglesh ^c   Gupta, Garima ^a   Boopathi, Mannan ^a   Gupta, Pallavi ^c   Chauhan, Vinita ^c   Tomar, Arvind ^c   Singh, Lokendra ^b   Dhaked, Ram Kumar ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^b Defence R D Organization   (India)

^c NONE

Author keywords

Antibody isotyping; Botulinum neurotoxin; GT 1b; HCC subunit; Renaturation; SPR

Indexed keywords

BOTULINUM TOXIN A; FREUND ADJUVANT; GANGLIOSIDE GT 1B; GUANIDINE; IMIDAZOLE; IMMUNOGLOBULIN A ANTIBODY; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2A ANTIBODY; IMMUNOGLOBULIN G2B ANTIBODY; IMMUNOGLOBULIN G3 ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; MERCAPTOETHANOL; NICKEL; RECOMBINANT BOTULINUM TOXIN A C TERMINAL HEAVY CHAIN BETA TREFOIL FOLD SUBDOMAIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY AFFINITY; ANTIBODY BLOOD LEVEL; ANTIBODY RESPONSE; ANTIBODY TITER; ARTICLE; BOTULISM; CELL INCLUSION; CONTROLLED STUDY; DELETION MUTANT; DRUG BINDING; DRUG PURIFICATION; DRUG PURITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; IMMUNOPROPHYLAXIS; MOLECULAR CLONING; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN REFOLDING; PROTEIN RENATURATION; SOLUBILIZATION; SURFACE PLASMON RESONANCE; VACCINE FAILURE; VACCINE PRODUCTION;

ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL VACCINES; BINDING SITES; BOTULINUM TOXINS, TYPE A; CLONING, MOLECULAR; CLOSTRIDIUM BOTULINUM; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GANGLIOSIDES; HISTIDINE; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MICE; OLIGOPEPTIDES; RECOMBINANT PROTEINS; SEQUENCE DELETION; SURFACE PLASMON RESONANCE;

ANIMALIA; ESCHERICHIA COLI;

EID: 84860586315     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986612800191008     Document Type: Article

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