2-aminobenzamide and 2-aminobenzoic acid as new MALDI matrices inducing radical mediated in-source decay of peptides and proteins

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 3, 2012, Pages 469-474

  Smargiasso, Nicolas ^a   Quinton, Loic ^a   De Pauw, Edwin ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Fragmentation; In Source decay; MALDI; Peptides; Proteins

Indexed keywords

2-AMINOBENZAMIDE; 2-AMINOBENZOIC ACID; ANALYTES; DISULFIDE BRIDGE; FRAGMENTATION; HYDROGEN RADICAL; IN-SOURCE DECAYS; MALDI; TOPDOWN;

AMINO ACIDS; COVALENT BONDS; HYDROGEN; IONS; PROTEINS;

PEPTIDES;

2 AMINOBENZAMIDE; A ION; ANTHRANILIC ACID; C ION; CALCITONIN; HYDROGEN RADICAL; ION; MYOGLOBIN; PEPTIDE; PROTEIN; RADICAL; UNCLASSIFIED DRUG; X ION; Y ION; Z ION; ANTHRANILIC ACID DERIVATIVE; DISULFIDE; PROTON; SERUM ALBUMIN;

AMINO ACID SEQUENCE; ARTICLE; CALCULATION; CHEMICAL PARAMETERS; DISULFIDE BOND; LASER; MATRIX ASSISTED LASER DESORPTION IONIZATION IN SOURCE DECAY; MOLECULAR WEIGHT; PROTEIN ANALYSIS; REDUCTION; CHEMISTRY; HUMAN; MASS SPECTROMETRY; METHODOLOGY; PROTEIN CONFORMATION;

ANTHRANILIC ACIDS; DISULFIDES; HUMANS; MYOGLOBIN; PEPTIDES; PROTEIN CONFORMATION; PROTEINS; PROTONS; SERUM ALBUMIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84860569521     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0307-5     Document Type: Article

References (32)

1. Brown, R.S., Lennon, J.J.: Sequence-specific fragmentation of matrixassisted laser-desorbed protein/peptide ions. Anal. Chem. 67(21), 3990-3999 (1995)
2. Takayama, M.: In-source decay characteristics of peptides in matrixassisted laser desorption/ionization time-of-flight mass spectrometry. J. Am. Soc. Mass Spectrom. 12(4), 420-427 (2001)
3. Sakakura, M., Takayama, M.: In-source decay and fragmentation characteristics of peptides using 5-aminosalicylic acid as a matrix in matrix-assisted laser desorption/ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 21, 979-988 (2010)
4. Demeure, K., Quinton, L., Gabelica, V., De Pauw, E.: Rational selection of the optimum MALDI matrix for top-down proteomics by in-source decay. Anal. Chem. 79(22), 8678-8685 (2007)
5. Resemann, A., Wunderlich, D., Rothbauer, U., Warscheid, B., Leonhardt, H., Fuchser, J., Kuhlmann, K., Suckau, D.: Top-down de novo protein sequencing of a 13.6 kDa camelid single heavy chain antibody by matrix-assisted laser desorption ionization-time-of-flight/ time-of-flight mass spectrometry. Anal. Chem. 82(8), 3283-3292 (2010)
6. Suckau, D., Resemann, A.: T3-sequencing: targeted characterization of the N- and C-termini of undigested proteins by mass spectrometry. Anal. Chem. 75(21), 5817-5824 (2003)
7. Wuhrer, M., Deelder, A.M.: Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: Targeted characterization of specific parts of the glycan structure. Rapid Commun. Mass Spectrom. 20(6), 943-951 (2006)
8. Yamagaki, T., Nakanishi, H.: Post-source decay fragmentation analyses of linkage isomers of Lewis-type oligosaccharides in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: Combined in-source decay/post-source decay experiments and relative ion abundance analysis. J. Mass Spectrom. 35(11), 1300-1307 (2000)
9. Yamagaki, T., Suzuki, H., Tachibana, K.: In-source and postsource decay in negative-ion matrix-assisted laser desorption/ionization timeof- flight mass spectrometry of neutral oligosaccharides. Anal. Chem. 77 (6), 1701-1707 (2005)
10. Bashir, S., Giannakopulos, A.E., Derrick, P.J., Critchley, P., Bottrill, A., Padley, H.J.: Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. A comparison of fragmentation patterns of linear dextran obtained by in-source decay, post-source decay, and collisioninduced dissociation and the stability of linear and cyclic glucans studied by in-source decay. Eur. J. Mass Spectrom. (Chichester, Eng) 10(1), 109-120 (2004)
11. Kameyama, A., Kaneda, Y., Yamanaka, H., Yoshimine, H., Narimatsu, H., Shinohara, Y.: Detection of oligosaccharides labeled with cyanine dyes using matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 76(15), 4537-4542 (2004)
12. Smargiasso, N., De Pauw, E.: Optimization of matrix conditions for the control of MALDI in-source decay of permethylated glycans. Anal. Chem. 82(22), 9248-9253 (2010)
13. Wang, B.H., Hopkins, C.E., Belenky, A.B., Cohen, A.S.: Sequencing of modified oligonucleotides using in-source fragmentation and delayed pulsed ion extraction matrix-assisted laser desorption ionization time-offlight mass spectrometry. Int. J. Mass Spectrom. 169, 331-350 (1997)
14. Suckau, D., Resemann, A.: MALDI top-down sequencing: Calling Nand C-terminal protein sequences with high confidence and speed. J. Biomol. Tech. 20(5), 258-262 (2009)
15. Schulz, E., Karas, M., Rosu, F., Gabelica, V.: Influence of the matrix on analyte fragmentation in atmospheric pressure MALDI. J. Am. Soc. Mass Spectrom. 17(7), 1005-1013 (2006)
16. Gabelica, V., Schulz, E., Karas, M.: Internal energy build-up in matrixassisted laser desorption/ionization. J. Mass Spectrom 39(6), 579-593 (2004)
17. Demeure, K., Gabelica, V., De Pauw, E.A.: New advances in the understanding of the in-source decay fragmentation of peptides in MALDI-TOF-MS. J. Am. Soc. Mass Spectrom. 21(11), 1906-1917 (2010)
18. Takayama, M.: N-C-→ bond cleavage of the peptide backbone via hydrogen abstraction. J. Am. Soc. Mass Spectrom. 12(9), 1044-1049 (2001)
19. Kocher, T., Engstrom, A., Zubarev, R.A.: Fragmentation of peptides in MALDI in-source decay mediated by hydrogen radicals. Anal. Chem. 77(1), 172-177 (2005)
20. Lennon, J.J., Walsh, K.A.: Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry. Protein Sci. 8(11), 2487-2493 (1999)
21. Netz, D.J., Pohl, R., Beck-Sickinger, A.G., Selmer, T., Pierik, A.J., Bastos Mdo, C., Sahl, H.G.: Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus. J. Mol. Biol. 319(3), 745-756 (2002)
22. Patterson, S.D., Katta, V.: Prompt fragmentation of disulfide-linked peptides during matrix-assisted laser desorption ionization mass spectrometry. Anal. Chem. 66(21), 3727-3732 (1994)
23. Quinton, L., Demeure, K., Dobson, R., Gilles, N., Gabelica, V., De Pauw, E.: New method for characterizing highly disulfide-bridged peptides in complex mixtures: Application to toxin identification from crude venoms. J. Proteome Res. 6(8), 3216-3223 (2007)
24. Schnaible, V., Wefing, S., Resemann, A., Suckau, D., Bucker, A., Wolf-Kummeth, S., Hoffmann, D.: Screening for disulfide bonds in proteins by MALDI in-source decay and LIFT-TOF/TOF-MS. Anal. Chem. 74(19), 4980-4988 (2002)
25. Deleuze, C., De Pauw, E., Quinton, L.: Selective reduction of C=C double bonds in matrix-assisted laser desorption/ionization time-offlight mass spectrometry of microcystins. Eur. J. Mass Spectrom. (Chichester, Eng) 16(1), 91-99 (2010)
26. Fukuyama, Y., Iwamoto, S., Tanaka, K.: Rapid sequencing and disulfide mapping of peptides containing disulfide bonds by using 1,5-diaminonaphthalene as a reductive matrix. J. Mass Spectrom. 41(2), 191-201 (2006)
27. Asakawa, D., Takayama, M.: C(→)-C bond cleavage of the peptide backbone in MALDI in-source decay using salicylic acid derivative matrices. J. Am. Soc. Mass Spectrom. 22(7), 1224-1233 (2011)
28. Takayama, M., Tsugita, A.: Sequence information of peptides and proteins with in-source decay in matrix assisted laser desorption/ ionization-time of flight-mass spectrometry. Electrophoresis 21(9), 1670-1677 (2000)
29. Zubarev, R.A., Kjeldsen, F., Haselmann, K.F., Budnik, B.A., Jensen, F.: Dissociative capture of hot (3-13 eV) electrons by polypeptide polycations: An efficient process accompanied by secondary fragmentation. Chem. Phys. Lett. 356(3/4), 201-206 (2002)
30. Johnson, R.S., Martin, S.A., Biemann, K.: Collision-induced fragmentation of (M+H)+ions of peptides-side-chain specific sequence ions. Int. J. Mass Spectrom. Ion Processes 86, 137-154 (1988)
31. Diedrich, J. K., Julian, R. R. Facile identification of phosphorylation sites in peptides by radical directed dissociation. Anal. Chem. 83(17), 6818-6826 (2011)
32. Kruger, N.A., Zubarev, R.A., Horn, D.M., McLafferty, F.W.: Electron capture dissociation of multiply charged peptide cations. Int. J. Mass Spectrom. 187, 787-793 (1999)