MALDI top-down sequencing: Calling N- and C-terminal protein sequences with high confidence and speed

Journal of Biomolecular Techniques

Volumn 20, Issue 5, 2009, Pages 258-262

  Suckau, Detlev ^a   Resemann, Anja ^a  

^a BRUKER DALTONIK GMBH   (Germany)

Author keywords

Edman sequencing; MALDI TOF; Protein QC; Top down protein sequencing

Indexed keywords

HYBRID PROTEIN; PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN STRUCTURE;

ALGORITHMS; COMPUTATIONAL BIOLOGY; DATABASES, PROTEIN; FUNGAL PROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 77951049022     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Hardouin J. Protein sequence information by matrix-assisted laser desorption/ionization in-source decay mass spectrometry. Mass Spectrom Rev 2007;26:672-682.
2. Suckau D, Resemann A. T3-sequencing: targeted characterization of the N- and C-termini of undigested proteins by mass spectrometry. Anal Chem 2003;75:5817-5824.
3. Suckau D, Resemann A, Schuerenberg M, Hufnagel P, Franzen J, Holle A. A novel MALDI LIFT-TOF/TOF mass spectrometer for proteomics. Anal Bioanal Chem 2003;376:952-965.
4. Anonymous Participant. ABRF-PRG-08 Study. http://www.abrf.org/ ResearchGroups/Proteomics/Studies/27960.pdf. Accessed September 2009.
5. ABRF Proteomics Research Group. PRG08 Poster. http://www.abrf.org/ ResearchGroups/Proteomics/Studies/PRG08-poster.pdf Accessed September 2009.