Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry

Protein Science

Volumn 8, Issue 11, 1999, Pages 2487-2493

  Lennon, John J ^a,b   Walsh, Kenneth A ^b  

^a Large Scale Biology Corporation   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

Delayed ion extraction; In source decay; MALDI; Posttranslational modifications; Protein sequencing

Indexed keywords

PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; DESORPTION; MASS SPECTROMETRY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

EID: 0032725664     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.11.2487     Document Type: Article

References (17)

1. Annan RS, Carr SA. 1996. Phosphopeptide analysis by matrix-assisted laser desorption time-of-flight mass spectrometry. Anal Chem 68:3413-3421.
2. Biemann K. 1988. Contributions of mass spectrometry to peptide and protein structure. Biomed Environ Mass Spectrom 16:99-111.
3. Brown RS, Carr BL, Lennon JJ. 1996. Factors that influence the observed fast fragmentation of peptides in matrix-assisted laser desorption. J Am Soc Mass Spectrom 7:225-232.
4. Brown RS, Lennon JJ. 1995. Sequence-specific fragmentation of matrix-assisted laser-desorbed protein/peptide ions. Anal Chem 67:3990-3999.
5. Carr SA, Huddleston MJ, Annan RS. 1996. Selective detection and sequencing of phosphopeptides at the femtomole level by mass spectrometry. Anal Biochem 239:180-192.
6. Covey T, Sushan R, Bonner R, Schröder W, Hucho F. 1991. LC/MS and LC/MS/MS screening for the sites of posttranslational modification in proteins. In: Jörnvall H, Höög JO, Gustavsson, eds. Methods in protein sequence analysis. Basel: Birkhäuser Verlag. pp 249-256.
7. Graves DJ, Martin BL, Wang JH. 1994. Co- and post-translational modification of proteins. Chemical principles and biological effects. New York: Oxford University Press.
8. Huddleston MJ, Annan RS, Bean MF, Carr SA. 1993. Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/ mass spectrometry. J Am Soc Mass Spectrom 4:710-717.
9. Karas M, Hillenkamp F. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10000 daltons. Anal Chem 60:2299-2301.
10. Katta V, Chow DT, Rohde MF. 1998. Applications of in-source fragmentation of protein ions for direct sequence analysis by delayed extraction MALDI-TOF mass spectrometry. Anal Chem 70:4410-4416.
11. Kaufmann R, Kirsch D, Spengler B. 1994. Sequencing of peptides in a time-of-flight mass spectrometer: Evaluation of postsource decay following matrix-assisted laser desorption ionization (MALDI). Int J Mass Spectrom Ion Processes 131:355-385.
12. Kaufmann R, Spengler B, Lützenkirchen F. 1993. Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization. Rapid Comm Mass Spectrom 7:902-910.
13. Lennon JJ, Walsh KA. 1997. Direct sequence analysis of proteins by in-source fragmentation during delayed ion extraction. Protein Sci 6:2446-2453.
14. Reiher DC, Brown RS, Weinberger S, Kenny J, Bailey J. 1998. Unknown peptide sequencing using matrix-assisted laser desorption/ionization and in-source decay. Anal Chem 70:1214-1222.
15. Taylor JA, Walsh KA, Johnson RS. 1996. Sherpa: A Macintosh-based expert system for the interpretation of ESI LC/MS and MS/MS of protein digests. Rapid Comm Mass Spectrom 10:679-687.
16. Zubarev RA, Kelleher NL, McLafferty FW. 1998. Electron capture dissociation of multiply charged protein cations. A nonergodic process. J Am Chem Soc 120:3265-3266.
17. Zubarev RA, Kruger NA, Fridriksson EK, Lewis MA, Horn DM, Carpenter BK, McLafferty FW. 1999. Electron capture dissociation of gaseous multiply-charged proteins is favored at disulfide bonds and other sites of high hydrogen atom affinity. J Am Chem Soc 121:2857-2862.