Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage

Protein Engineering

Volumn 12, Issue 2, 1999, Pages 179-184

  Humphreys, David P ^a   Smith, Bryan J ^a   King, Lloyd M ^a   West, Shauna M ^a   Reeks, Dominic G ^a   Stephens, Paul E ^a  

^a UCB PHARMA   (Belgium)

Author keywords

Copper (II); Cupric; Fab'; FLAG peptide; Protein cleavage

Indexed keywords

COPPER; HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G1; PEPTIDE; RECOMBINANT PROTEIN;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE INSERTION SEQUENCE; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS;

EID: 0032939420     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.2.179     Document Type: Article

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