Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin

Protein Engineering, Design and Selection

Volumn 25, Issue 5, 2012, Pages 251-259

  Back, J W ^a   Frisch, C ^b   Van Pee, K ^c   Boschert, V ^c   Van Vught, R ^a,d   Puijk, W ^a   Mueller, T D ^c   Knappik, A ^b   Timmerman, P ^a,e  

^a Pepscan Therapeutics   (Netherlands)

^b Division of MorphoSys   (Germany)

^c UNIVERSITY OF WÜRZBURG   (Germany)

^d UTRECHT UNIVERSITY   (Netherlands)

^e UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

cystine knot; epitope specific antibodies; oxidative folding; sclerostin

Indexed keywords

CYSTINE KNOT; LINEAR PEPTIDES; NATIVE PROTEINS; NEGATIVE REGULATORS; OXIDATIVE FOLDING; PHAGE DISPLAY LIBRARIES; PRIMARY SEQUENCES; PROTEIN DOMAINS; RECOGNITION SPECIFICITY; REFOLDING; SCLEROSTIN; SYNTHETIC PEPTIDE; WNT PATHWAY;

ANTIGENS; PEPTIDES; RECOMBINANT PROTEINS;

ANTIBODIES;

ANTIBODY; CYSTINE; SCLEROSTIN; WNT PROTEIN;

AMINO ACID SEQUENCE; ANTIBODY STRUCTURE; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NEGATIVE FEEDBACK; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SURFACE PLASMON RESONANCE;

ANIMALS; CYSTINE; EPITOPE MAPPING; EPITOPES; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MICE; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PEPTIDE LIBRARY; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEINS; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; WNT SIGNALING PATHWAY;

EID: 84860468166     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs012     Document Type: Article

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