Molecular studies to identify the Fusarium species responsible for HT-2 and T-2 mycotoxins in UK oats

International Journal of Food Microbiology

Volumn 156, Issue 2, 2012, Pages 168-175

  Edwards, Simon G ^a   Imathiu, Samuel M ^a,b   Ray, Rumiana V ^a,c   Back, Matthew ^a   Hare, Martin C ^a  

^a HARPER ADAMS UNIVERSITY   (United Kingdom)

^b Kenya Polytechnic University College   (Kenya)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Cereals; Fusarium head blight; Fusarium langsethiae; Trichothecenes

Indexed keywords

FUNGAL DNA; HT 2 TOXIN; NIVALENOL; T 2 TOXIN; TRICHOTHECENE;

ARTICLE; CEREAL; CONTROLLED STUDY; CORRELATION ANALYSIS; FLOUR; FOOD CONTAMINATION; FUNGUS ISOLATION; FUSARIUM; FUSARIUM LANGSETHIAE; FUSARIUM POAE; FUSARIUM SPOROTRICHIOIDES; LIMIT OF QUANTITATION; MICROBIOLOGICAL EXAMINATION; MULTIPLE REGRESSION; NONHUMAN; OAT; REAL TIME POLYMERASE CHAIN REACTION; REGRESSION ANALYSIS; UNITED KINGDOM;

AVENA SATIVA; CEREALS; DNA, FUNGAL; FUSARIUM; GREAT BRITAIN; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; T-2 TOXIN; TRICHOTHECENES;

AVENA; FUNGI; FUSARIUM; FUSARIUM LANGSETHIAE; FUSARIUM POAE; FUSARIUM SPOROTRICHIOIDES;

EID: 84860377370     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2012.03.020     Document Type: Article

References (43)

1. Altomare C., Petrini O., Logrieco A., Bottalico A. Taxonomic relationships among the toxigenic species Fusarium acuminatum, Fusarium sporotrichioides and Fusarium tricinctum by isozyme analysis and RAPD assay. Canadian Journal of Botany-Revue Canadienne De Botanique 1997, 75:1674-1684.
2. Anon. Commission Regulation (EC) No. 1881/2006 setting maximum levels of certain contaminants in foodstuffs. Official Journal of the European Union 2006, L364:5-24.
3. Aoki T., O'Donnell K. Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the Group 1 population of F. graminearum. Mycologia 1999, 91:597-609.
4. Bottalico A., Perrone G. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology 2002, 108:611-624.
5. Burgess L.W., Summerell B.A. Taxonomy of Fusarium: Fusarium armeniacum stat & comb. nov. Mycotaxon 2000, 75:347-348.
6. Doohan F.M., Parry D.W., Jenkinson P., Nicholson P. The use of species specific PCR based assays to analyse fusarium ear blight of wheat. Plant Pathology 1998, 47:197-205.
7. Doohan F.M., Parry D.W., Nicholson P. Fusarium ear blight of wheat: the use of quantitative PCR and visual disease assessment in studies of disease control. Plant Pathology 1999, 48:209-217.
8. Edwards S.G. Fusarium mycotoxin content of UK organic and conventional oats. Food Additives and Contaminants. Part A, Chemistry, Analysis, Control, Exposure and Risk Assessment 2009, 26:1063-1069.
9. Fredlund E., Gidlund A., Pettersson H., Olsen M., Borjesson T. Real-time PCR detection of Fusarium species in Swedish oats and correlation to T-2 and HT-2 toxin content. World Mycotoxin Journal 2010, 3:77-88.
10. Gilbert J., Tekauz A. Effects of Fusarium head blight and seed treatment on germination, emergence, and seedling vigour of spring. Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie 1995, 17:252-259.
11. Goswami R.S., Kistler H.C. Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology 2004, 5:515-525.
12. Halstensen A.S., Nordby K.-C., Eduard W., Klemsdal S.S. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. Journal of Environmental Monitoring 2006, 8:1235-1241.
13. Halstensen A.S., Nordby K.-C., Klemsdal S.S., Elen O., Clasen P.-E., Eduard W. Toxigenic Fusarium spp. as determinants of trichothecene mycotoxins in settled grain dust. Journal of Occupational and Environmental Hygiene 2006, 3:651-659.
14. Imathiu, S.M. 2008. Fusarium langsethiae infection and mycotoxin production in oats. PhD Thesis. PhD Thesis, Harper Adams University College, UK.
15. Imathiu S.M., Hare M.C., Ray R.V., Back M., Edwards S.G. Evaluation of pathogenicity and aggressiveness of F. langsethiae on oat and wheat seedlings relative to known seedling blight pathogens. European Journal of Plant Pathology 2010, 126:203-216.
16. Infantino A., Pucci N., Conca G., Santori A. First report of Fusarium langsethiae on durum wheat kernels in Italy. Plant Disease 2007, 91:1362.
17. Jurado M., Vazquez C., Marin S., Sanchis V., Gonzalez-Jaen M.T. PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize. Systematic and Applied Microbiology 2006, 29:681-689.
18. Knutsen A.K., Torp M., Holst-Jensen A. Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene. International Journal of Food Microbiology 2004, 95:287-295.
19. Langseth W., Rundberget T. The occurrence of HT-2 toxin and other trichothecenes in Norwegian cereals. Mycopathologia 1999, 147:157-165.
20. Leslie J.F., Summerell B.A. The Fusarium Laboratory Manual 2006, Blackwell Publishing, Ames, Iowa.
21. McCartney H.A., Foster S.J., Fraaije B.A., Ward E. Molecular diagnostics for fungal plant pathogens. Pest Management Science 2003, 59:129-142.
22. Nicholson P., Simpson D.R., Weston G., Rezanoor H.N., Lees A.K., Parry D.W., Joyce D. Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 1998, 53:17-37.
23. Nicholson P., Simpson D.R., Wilson A.H., Chandler E., Thomsett M. Detection and differentiation of trichothecene and enniatin-producing Fusarium species on small-grain cereals. European Journal of Plant Pathology 2004, 110:503-514.
24. Nicolaisen M., Supronien S., Nielsen L.K., Lazzaro I., Spliid N.H., Justesen A.F. Real-time PCR for quantification of eleven individual Fusarium species in cereals. Journal of Microbiological Methods 2009, 76:234-240.
25. Nielsen L.K., Jensen J.D., Nielsen G.C., Jensen J.E., Spliid N.H., Thomsen I.K., Justesen A.F., Collinge D.B., Jorgensen L.N. Fusarium head blight of cereals in Denmark: species complex and related mycotoxins. Phytopathology 2011, 101:960-969.
26. Osborne L.E., Stein J.M. Epidemiology of Fusarium head blight on small-grain cereals. International Journal of Food Microbiology 2007, 119:103-108.
27. Parry D.W., Nicholson P. Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology 1996, 45:383-391.
28. Parry D.W., Jenkinson P., McLeod L. Fusarium ear blight (Scab) in small-grain cereals - a review. Plant Pathology 1995, 44:207-238.
29. Sinha R.C., Savard M.E. Concentration of deoxynivalenol in single kernels and various tissues of wheat heads. Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie 1997, 19:8-12.
30. Snijders C.H.A. Resistance in wheat to Fusarium infection and trichothecene formation. Toxicology Letters 2004, 153:37-46.
31. Thrane U., Adler A., Clasen P.E., Galvano F., Langseth W., Logrieco A., Nielsen K.F., Ritieni A. Diversity in metabolite production by Fusarium langsethiae, Fusarium poae, and Fusarium sporotrichioides. International Journal of Food Microbiology 2004, 95:257-266.
32. Torp M., Langseth W. Production of T-2 toxin by a Fusarium resembling Fusarium poae. Mycopathologia 1999, 147:89-96.
33. Torp M., Nirenberg H.I. Fusarium langsethiae sp. nov. on cereals in Europe. International Journal of Food Microbiology 2004, 95:247-256.
34. Walsh P.S., Metzger D.A., Higuchi R. Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 1991, 10:506-513.
35. White T.J., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 1990, 315-322. Academic Press, San Diego, California. M.A. Innis (Ed.).
36. Wilson A., Simpson D., Chandler E., Jennings P., Nicholson P. Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiology Letters 2004, 233:69-76.
37. Wing N., Lauren D.R., Bryden W.L., Burgess L.W. Toxicity and trichothecene production by Fusarium acuminatum subsp. acuminatum and Fusarium acuminatum subsp. armeniacum. Natural Toxins 1993, 1:229-234.
38. Xu X.M., Parry D.W., Nicholson P., Thomsett M.A., Simpson D., Edwards S.G., Cooke B.M., Doohan F.M., Brennan J.M., Moretti A., Tocco G., Mule G., Hornok L., Giczey G., Tatnell J. Predominance and association of pathogenic fungi causing Fusarium ear blight in wheat in four European countries. European Journal of Plant Pathology 2005, 112:143-154.
39. Yli-Mattila T. Ecology and evolution of toxigenic Fusarium species in cereals in northern Europe and Asia. Journal of Plant Pathology 2010, 92:7-18.
40. Yli-Mattila T., Paavanen-Huhtala S., Parikka P., Hietaniemi V., Jestoi M., Rizzo A. Toxigenic fungi and mycotoxins in Finnish cereals. An Overview on Toxigenic Fungi and Mycotoxins in Europe 2004, 83-100. Kluwer Academic Publishers, The Netherlands. A. Logrieco, A. Visconti (Eds.).
41. Yli-Mattila T., Paavanen-Huhtala S., Parikka P., Konstantinova P., Gagkaeva T.Y. Molecular and morphological diversity of Fusarium species in Finland and north-western Russia. European Journal of Plant Pathology 2004, 110:573-585.
42. Yli-Mattila T., Paavanen-Huhtala S., Jestoi M., Parikka P., Hietaniemi V., Gagkaeva T., Sarlin T., Haikara A., Laaksonen S., Rizzo A. Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. Archives of Phytopathology and Plant Protection 2008, 41:243-260.
43. Yli-Mattila T., Ward T.J., O'Donnell M., Proctor R.H., Burkin A.A., Kononenko G.P., Gavrilova O.P., Aoki T., McCormick S.P., Gagkaeva T.Y. Fusarium sibiricum sp. nov., a novel type A trichothecene-producing Fusarium from northern Asia closely related to F. sporotrichioides and F. langsethiae. International Journal of Food Microbiology 2011, 147:58-68.