Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays

Physiological and Molecular Plant Pathology

Volumn 53, Issue 1, 1998, Pages 17-37

  Nicholson, P ^a   Simpson, D R ^a   Weston, G ^a   Rezanoor, H N ^a   Lees, A K ^a,b   Parry, D W ^a,c   Joyce, D ^a,d  

^a JOHN INNES CENTRE   (United Kingdom)

^b SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^c HORTICULTURE RESEARCH INTERNATIONAL   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAY; DNA AMPLIFICATION; FUNGAL DNA; FUNGUS DETECTION; PLANT DISEASE; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; TRICHOTHECENE; VIRULENCE; WHEAT;

FUSARIUM CULMORUM; FUSARIUM GRAMINEARUM;

EID: 0031672867     PISSN: 08855765     EISSN: None     Source Type: Journal    
DOI: 10.1006/pmpp.1998.0170     Document Type: Article

References (33)

1. Bateman GL, Kwaśna H, Ward E. 1996. Relationships among Fusarium spp. estimated by comparing restriction fragment length polymorphisms in polymerase chain reaction-amplified nuclear rDNA. Canadian Journal of Microbiology 42: 1232-1240.
2. Cook RJ. 1968. Fusarium root and foot rot of cereals in the Pacific Northwest. Phytopathology 58: 127-131.
3. Desjardins AE, Hohn TM. 1997. Mycotoxins in plant pathogenesis. Molecular-Plant Microbe Interactions 10: 147-152.
4. Desjardins AE, Proctor RH, Bai G, McCormick SP, Shaner G, Buechley G, Hohn TM. 1996. Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Molecular Plant-Microbe Interactions 9: 775-781.
5. Doohan FM, Parry DW, Jenkinson P, Nicholson P. 1998. The use of species-specific PCR-based assays to analyse fusarium ear blight of wheat. Plant Pathology 47: 197-205.
6. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 1991. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Research 19: 4008.
7. Edel V, Steinberg C, Gautheron N, Alabouvette C. 1996. Evaluation of restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA for the identification of Fusarium species. Mycological Research 101: 179-187.
8. Feinberg AP, Vogelstein B. 1984. Addendum: a technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137: 266-267.
9. Förster E. 1994. An improved general method to generate internal standards for competitive PCR. Biotechniques 16: 18-20.
10. Goulds A, Polley RW. 1990. Assessment of eyespot and other stem base diseases of winter wheat and winter barley. Mycological Research 94: 819-822.
11. Guadet J, Julien J, Lafay JF, Brygoo Y. 1989. Phylogeny of some Fusarium species, as determined by large-subunit rDNA sequence comparison. Molecular and Biological Evolution 6: 227-242.
12. Hu X, Nazar RN, Robb J. 1993. Quantification of Verticillium biomass in wilt disease development. Physiological and Molecular Plant Pathology 42: 23-36.
13. Joffe AZ. 1986. Fusarium Species : Their biology and toxicology. New York: John Wiley & Sons.
14. Lincoln SE, Daly MJ, Lander ES. 1991. Primer. The Whitehead Institute for Biomedical Research.
15. Moukhamedov R, Hu X, Nazar RN, Robb J. 1994. Use of polymerase chain reaction-amplified ribosomal intergenic sequences for the diagnosis of Verticillium tricorpus. Phytopathology 84: 256-259.
16. Nicholson P, Rezanoor HN. 1994. The use of random amplified polymorphic DNA to identify pathotype and detect variation in Pseudocercosporella herpotrichoides. Mycological Research 98: 13-21.
17. Nicholson P, Parry DW. 1996. Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot, in wheat. Plant Pathology 45: 872-883.
18. Nicholson P, Lees AK, Maurin N, Parry DW, Rezanoor HN. 1996. Development of a PCR assay to identify and quantify Microdochium nivale var. nivale and Microdochium nivale var. majus in wheat. Physiological and Molecular Plant Pathology 48: 257-271.
19. Nicholson P, Rezanoor HN, Simpson DR, Joyce D. 1997. Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay. Plant Pathology 46: 842-856.
20. Oullet T, Seifert KA. 1993. Genetic characterisation of Fusarium graminearum strains using RAPD and PCR amplification. Phytopathology 83: 1003-1007.
21. Parry DW, Nicholson P. 1996. Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology 45: 383-391.
22. Parry DW, Jenkinson P, McLeod L. 1995. Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathology 44: 207-238.
23. Pettitt TR, Parry DW, Policy RW. 1993. Improved estimation of the incidence of Microdochium nivale in winter wheat in England and Wales during 1992, by use of benomyl agar. Mycological Research 97: 1172-1174.
24. Polley RW, Turner JA. 1995. Surveys of stem base diseases and Fusarium ear diseases in winter wheat in England, Wales and Scotland (1989-1990). Annals of Applied Biology 126: 45-59.
25. Reed KC, Mann DA. 1985. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Research 13: 7207-7221.
26. Sambrook J, Fritsch EF, Maniatis TA. 1989. Molecular cloning: A laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
27. Schilling AG. 1996. Characterisation and differentiation of the cereal pathogens Fusarium culmorum and F. graminearum by PCR-based molecular markets. PhD thesis, Hohenheim University, Germany.
28. Schilling AG, Möller EM, Geiger HH. 1996. Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum and F. avenaceum. Phytopathology 86: 515-522.
29. Snedecor GW, Cochran WG. 1980. Statistical Methods. The Iowa State University Press, Iowa, USA.
30. Turner AS, Lees AK, Rezanoor HN, Nicholson P. 1998. Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to F. tricinctum. Plant Pathology (in press).
31. Williams JGK, Kubelik AR, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531-6535.
32. Wales R, Newman BJ, Rose SA, Pappin D, Gray JC. 1989. Characterisation of cDNA clones encoding the extrinsic 23 kDa polypeptide of the oxygen-evolving complex of photosystem-II in pea. Plant Molecular Biology 13: 573-582.
33. Zadoks JC, Chang TT, Konzak CF. 1974. A decimal code for the growth stages of cereals. Weed Research 14: 415-421.