Optimized procedure for expression and renaturation of recombinant human bone morphogenetic protein-2 at high protein concentrations

Molecular Biology Reports

Volumn 37, Issue 7, 2010, Pages 3089-3095

  Zhang, Hongbin ^a   Wu, Jie ^b   Zhang, Yu ^a   Fu, Na ^a   Wang, Jie ^a   Zhao, Shujin ^a  

^a Guangzhou Liuhuaqiao Hospital   (China)

^b GUANGZHOU CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

Expression; Purification; Recombinant human bone morphogenetic protein 2; Renaturation

Indexed keywords

ALKALINE PHOSPHATASE; BUFFER; RECOMBINANT BONE MORPHOGENETIC PROTEIN 2; SEPHAROSE; UREA; WATER;

ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; DIALYSIS; ENZYME ACTIVITY; ESCHERICHIA COLI; MUSCLE; NONHUMAN; OSSIFICATION; OSTEOBLAST; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN RENATURATION; VASTUS INTERMEDIUS MUSCLE;

ALKALINE PHOSPHATASE; ANIMALS; BIOCHEMISTRY; BLOTTING, WESTERN; BONE MORPHOGENETIC PROTEINS; CELL LINE; FERMENTATION; HUMANS; MICE; MUSCLES; PLASMIDS; PROTEIN RENATURATION; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; TRANSFORMING GROWTH FACTOR BETA;

ESCHERICHIA COLI; PROKARYOTA;

EID: 77956975741     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9883-x     Document Type: Article

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