Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on histidine-tag chemistry

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 541-546

  Shimada, Josui ^a   Maruyama, Tatsuo ^a,b   Kitaoka, Momoko ^a   Kamiya, Noriho ^a   Goto, Masahiro ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

DNA aptamer; DNA enzyme conjugate; DNase I; Enzyme reaction; His tag; Reversible immobilization

Indexed keywords

ALKALINITY; AMINO ACIDS; CATALYSIS; CROSSLINKING; ENZYME IMMOBILIZATION; NICKEL COMPOUNDS; OLIGONUCLEOTIDES; PHOSPHATASES; RECOMBINANT PROTEINS;

DNA APTAMER; DNASE I; ENZYME REACTION; HIS-TAG; REVERSIBLE IMMOBILIZATION;

DNA;

APTAMER; DEOXYRIBONUCLEASE; DNA; ENZYME; NITRILOTRIACETIC ACID; POLYHISTIDINE TAG; THROMBIN;

ARTICLE; ASSAY; BINDING AFFINITY; BIOTINYLATION; CONJUGATE; DNA ENZYME CONJUGATE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; GENE AMPLIFICATION; MICROPLATE ASSAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

EID: 84859711910     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.028     Document Type: Article

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