Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system

Biotechnology Letters

Volumn 30, Issue 11, 2008, Pages 2001-2006

  Shimada, Josui ^a   Maruyama, Tatsuo ^a,b   Hosogi, Takuya ^a   Tominaga, Jo ^a   Kamiya, Noriho ^a   Goto, Masahiro ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

DNA aptamer; DNA protein conjugate; Enzymatic reaction; Histidine tag; Protein immobilization

Indexed keywords

CHEMISTRY; CHLORINE COMPOUNDS; GENES; NICKEL; NUCLEIC ACIDS; ORGANIC ACIDS; SENSORS;

APTAMER; DETECTION SYSTEMS; DNA APTAMER; DNA-PROTEIN CONJUGATE; ENZYMATIC REACTION; HIGH AFFINITY; HIS-TAG; HISTIDINE-TAG; NOVEL METHODS; PROTEIN IMMOBILIZATION; RECOMBINANT PROTEINS;

DNA;

ALKALINE PHOSPHATASE; APTAMER; DNA; HISTIDINE; NITRILOTRIACETIC ACID; PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; PROTEIN BINDING; REPRODUCIBILITY;

ALKALINE PHOSPHATASE; APTAMERS, NUCLEOTIDE; CHEMISTRY, ANALYTICAL; DNA; HISTIDINE; NITRILOTRIACETIC ACID; PROTEIN BINDING; PROTEINS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 52449088063     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9784-4     Document Type: Article

References (19)

1. MK Beissenhirtz I Willner 2006 DNA-based machines Org Biomol Chem 4 3392 3401
2. LC Bock LC Griffin JA Latham EH Vermaas JJ Toole 1992 Selection of single-stranded DNA molecules that bind and inhibit human thrombin Nature 355 564 566
3. C Boozer J Ladd S Chen Q Yu J Homola S Jiang 2004 DNA directed protein immobilization on mixed ssDNA/oligo(ethylene glycol) self-assembled monolayers for sensitive biosensor Anal Chem 76 6967 6972
4. DW Drolet L MoonMcDermott TS Romig 1996 An enzyme-linked oligonucleotide assay Nat Biotechnol 14 1021 1025
5. AD Ellington JW Szostak 1990 In vitro selection of RNA molecules that bind specific ligands Nature 346 818 822
6. JF Hainfeld WQ Liu CMR Halsey P Freimuth RD Powell 1999 Ni-NTA-gold clusters target his-tagged proteins J Struct Biol 127 185 198
7. T Maruyama T Hosogi M Goto 2007 Sequence-selective extraction of single-strand DNA using DNA-functionalized reverse micelles Chem Commun 43 4450 4452
8. CM Niemeyer 2001 Bioorganic applications of semisynthetic DNA-protein conjugates Chem Eur J 7 3189 3195
9. CM Niemeyer 2002 The developments of semisynthetic DNA-protein conjugates Trends Biotechnol 20 395 401
10. R Nutiu YF Li 2003 Structure-switching signaling aptamers J Am Chem Soc 125 4771 4778
11. A Saghatelian KM Guckian DA Thayer MR Ghadiri 2003 DNA detection and signal amplification via an engineered allosteric enzyme J Am Chem Soc 125 344 345
12. EL Schmid TA Keller Z Dienes H Vogel 1997 Reversible oriented surface immobilization of functional proteins on oxide surfaces Anal Chem 69 1979 1985
13. J Schmitt H Hess HG Stunnenberg 1993 Affinity purification of histidine-tagged proteins Mol Biol Rep 18 223 230
14. NC Seeman 2003 DNA in a material world Nature 421 427 431
15. S Takeda S Tsukiji T Nagamune 2004 Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation Bioorg Med Chem Lett 14 2407 2410
16. J Tominaga Y Kemori Y Tanaka T Maruyama N Kamiya M Goto 2007 An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides Chem Commun 4 401 403
17. C Tuerk L Gold 1990 Systematic evolution of ligands by exponential enrichment-RNA ligands to bacteriophage-T4 DNA-polymerase Science 249 505 510
18. S Tyagi FR Kramer 1996 Molecular beacons: probes that fluorescence upon hybridization Nat Biotechnol 14 303 308
19. H Yan SH Park G Finkelstein JH Reif TH LaBean 2003 DNA-templated self-assembly of protein arrays and highly conductive nanowires Science 301 1882 1884