A high-throughput fluorescence polarization anisotropy assay for the 70N domain of replication protein A

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 742-749

  Souza Fagundes, Elaine M ^a,b   Frank, Andreas O ^a   Feldkamp, Michael D ^a   Dorset, Daniel C ^a   Chazin, Walter J ^a   Rossanese, Olivia W ^a   Olejniczak, Edward T ^a   Fesik, Stephen W ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

ATRIP; Cancer; Checkpoint pathways; Fluorescence polarization anisotropy assay; High throughput screening; RPA70N

Indexed keywords

ANISOTROPY; CELLS; CHEMOTHERAPY; DISEASES; DNA; FLUORESCENCE; MAMMALS; PEPTIDES;

ATRIP; CANCER; CHECKPOINT PATHWAYS; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; RPA70N;

POLARIZATION;

ATR PROTEIN; FLUORESCEIN ISOTHIOCYANATE; MRE11 PROTEIN; PROTEIN ATRIP; PROTEIN P53; PROTEIN RAD9; REPLICATION FACTOR A; UNCLASSIFIED DRUG;

ARTICLE; DNA DAMAGE; DNA REPAIR; FLUORESCENCE POLARIZATION IMMUNOASSAY; HIGH THROUGHPUT SCREENING; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION;

MAMMALIA;

EID: 84859708704     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.025     Document Type: Article

References (22)

1. M.S. Wold, Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism, Annu. Rev. Biochem. 66 (1997) 61-92. (Pubitemid 27274652)
2. E. Fanning, V. Klimovich, A.R. Nager, A dynamic model for replication protein A (RPA) function in DNA processing pathways, Nucleic Acids Res. 34 (2006) 4126-4137. (Pubitemid 44542191)
3. K.A. Cimprich, D. Cortez, ATR: an essential regulator of genome integrity, Nat. Rev. Mol. Cell Biol. 9 (2008) 616-627. (Pubitemid 352032926)
4. X. Xu, S. Vaithiyalingam, G.G. Glick, D.A. Mordes, W.J. Chazin, D. Cortez, The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling, Mol. Cell. Biol. 28 (2008) 7345-7353.
5. H.L. Ball, M.R. Ehrhardt, D.A. Mordes, G.G. Glick, W.J. Chazin, D. Cortez, Function of a conserved checkpoint recruitment domain in ATRIP proteins, Mol. Cell. Biol. 27 (2007) 3367-3377. (Pubitemid 46691119)
6. G.G. Oakley, J.G. Glanzer, S.Q. Liu, Small molecule inhibitor of the RPA70 N-terminal protein interaction domain discovered using in silico and in vitro methods, Bioorg. Med. Chem. 19 (2011) 2589-2595.
7. J. Bartek, J. Bartkova, Z. Horejsi, K. Koed, A. Kramer, F. Tort, K. Zieger, P. Guldberg, M. Sehested, J.M. Nesland, C. Lukas, T. Orntoft, J. Lukas, DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis, Nature 434 (2005) 864-870. (Pubitemid 40558993)
8. M.R. Arkin, J.A. Wells, Small-molecule inhibitors of protein-protein interactions: progressing towards the dream, Nat. Rev. Drug Discov. 3 (2004) 301-317. (Pubitemid 38499758)
9. P.J. Hajduk, J. Greer, A decade of fragment-based drug design: strategic advances and lessons learned, Nat. Rev. Drug Discov. 6 (2007) 211-219. (Pubitemid 46344625)
10. S.B. Shuker, P.J. Hajduk, R.P. Meadows, S.W. Fesik, Discovering high-affinity ligands for proteins: SAR by NMR, Science 274 (1996) 1531-1534. (Pubitemid 26403929)
11. P.J. Hajduk, G. Sheppard, D.G. Nettesheim, E.T. Olejniczak, S.B. Shuker, R.P. Meadows, D.H. Steinman, G.M. Carrera, P.A. Marcotte, J. Severin, K. Walter, H. Smith, E. Gubbins, R. Simmer, T.F. Holzman, D.W. Morgan, S.K. Davidsen, J.B. Summers, S.W. Fesik, Discovery of potent nonpeptide inhibitors of stromelysin using SAR by NMR, J. Am. Chem. Soc. 119 (1997) 5818-5827. (Pubitemid 27305763)
12. J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer, New York, 2006.
13. B. Brutscher, P. Schanda, E. Kupce, SOFAST-HMQC experiments for recording two-dimensional heteronuclear correlation spectra of proteins within a few seconds, J. Biomol. NMR 33 (2005) 199-211. (Pubitemid 43118574)
14. Z.X. Wang, An exact mathematical expression for describing competitive-binding of 2 different ligands to a protein molecule, FEBS Lett. 360 (1995) 111-114.
15. Z.X. Wang, R.F. Jiang, A novel two-site binding equation presented in terms of the total ligand concentration, FEBS Lett. 392 (1996) 245-249.
16. R. Buchli, R.S. VanGundy, H.D. Hickman-Miller, C.F. Giberson, W. Bardet, W.H. Hildebrand, Development and validation of a fluorescence polarization-based competitive peptide-binding assay for HLA-A/0201: a new tool for epitope discovery, Biochemistry 44 (2005) 12491-12507. (Pubitemid 41324340)
17. Z. Nikolovska-Coleska, R. Wang, X. Fang, H. Pan, Y. Tomita, P. Li, P.P. Roller, K. Krajewski, N.G. Saito, J.A. Stuckey, S. Wang, Development and optimization of a binding assay for the XIAP BIR3 domain using fluorescence polarization, Anal. Biochem. 332 (2004) 261-273. (Pubitemid 39485734)
18. R. Macarron, R.P. Hertzberg, Design and implementation of high throughput screening assays, Mol. Biotechnol. 47 (2011) 270-285.
19. H.L. Ball, J.S. Myers, D. Cortez, ATRIP binding to replication protein A: single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation, Mol. Biol. Cell 16 (2005) 2372-2381. (Pubitemid 40632219)
20. C. Dalvit, NMR methods in fragment screening: theory and a comparison with other biophysical techniques, Drug Discov. Today 14 (2009) 1051-1057.
21. J. Kusba, V. Bogdanov, I. Gryczynski, J.R. Lakowicz, Theory of light quenching: effects on fluorescence polarization, intensity, and anisotropy decays, Biophys. J. 67 (1994) 2024-2040. (Pubitemid 24342075)
22. T.C. Turek-Etienne, E.C. Small, S.C. Soh, T.P.A. Xin, P.V. Gaitonde, E.B. Barrabee, R.F. Hart, R.W. Bryant, Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 kinases, 1 protease, and 1 phosphatase, J. Biomol. Screen. 8 (2003) 176-184.