Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production

Journal of Biological Chemistry

Volumn 287, Issue 15, 2012, Pages 12121-12131

  Zerbe, Philipp ^a   Chiang, Angela ^a   Yuen, Macaire ^a   Hamberger, Björn ^a   Hamberger, Britta ^a   Draper, Jason A ^b   Britton, Robert ^b   Bohlmann, Jörg ^a,c  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b SIMON FRASER UNIVERSITY   (Canada)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ABIES BALSAMEA; ABIETADIENE; ACTIVE SITE; ACTIVE SITE RESIDUES; BALSAM FIR; BIFUNCTIONAL; BIFUNCTIONAL ENZYMES; CARBOCATIONS; CLASS I; CLASS II; CYCLIZATIONS; DEPROTONATION REACTION; DIPHOSPHATES; DITERPENES; FRAGRANCE INDUSTRY; FULL-LENGTH CDNA; FUNCTIONAL CHARACTERIZATION; HIGH-THROUGHPUT; HOMOLOGY MODELING; METABOLITE PROFILING; REACTION MECHANISM; REACTION SEQUENCES; SYNTHASES; TRANSCRIPTOMES;

GENETIC ENGINEERING; OLEFINS; RESINS; TISSUE;

CYCLIZATION;

ABIETADIENE SYNTHASE; ASPARAGINE; CIS ABIENOL SYNTHASE; COMPLEMENTARY DNA; DITERPENE; DITERPENOID; FRAGRANCE; GLYCINE; LEUCINE; LEVOPIMARADIENE SYNTHASE; PHENYLALANINE; TRANSCRIPTOME; UNCLASSIFIED DRUG;

ABIES; ABIES BALSAMEA; AMINO ACID SEQUENCE; ARTICLE; BARK; CATALYSIS; CYCLIZATION; DNA BASE COMPOSITION; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME MECHANISM; HYDROXYLATION; MASS FRAGMENTOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CLEAVAGE; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN STRUCTURE; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS;

ABIES; AMINO ACID SUBSTITUTION; BIOCATALYSIS; CATALYTIC DOMAIN; DITERPENES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NAPHTHOLS; PERFUME; PHYLOGENY; PLANT BARK; PLANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANSCRIPTOME;

ABIES BALSAMEA; GYMNOSPERMAE;

EID: 84859487055     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.317669     Document Type: Article

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