Accurate quantitation of phospholamban phosphorylation by immunoblot

Analytical Biochemistry

Volumn 425, Issue 1, 2012, Pages 68-75

  Ablorh, Naa Adjeley ^a   Miller, Tyler ^a   Nitu, Florentin ^a   Gruber, Simon J ^a   Karim, Christine ^a   Thomas, David D ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Heart failure; Phospholamban; Phosphorylation; SERCA; Western blot

Indexed keywords

CELL PROLIFERATION; MAMMALS; SYNTHESIS (CHEMICAL);

BIOLOGICAL SAMPLES; HEART FAILURE; PHOSPHOLAMBAN; PLB PHOSPHORYLATION; SARCOPLASMIC RETICULUM; SERCA; SOLID PHASE PEPTIDE SYNTHESIS; WESTERN BLOTS;

PHOSPHORYLATION;

CALCIUM ION; PHOSPHOLAMBAN; PROTEIN ANTIBODY; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; SERINE;

ACCURACY; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOPLASM; DIASTOLE; ENZYME ACTIVATION; ENZYME INHIBITION; HEART MUSCLE CELL; HEART MUSCLE RELAXATION; IMMUNOBLOTTING; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; SARCOPLASMIC RETICULUM; SOLID PHASE SYNTHESIS; SWINE;

ANIMALIA; SUIDAE;

EID: 84859426518     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.028     Document Type: Article

References (54)

1. M. Heron, D.L. Hoyert, S.L. Murphy, J. Xu, K.D. Kochanek, and B. Tejada-Vera Deaths: final data for 2006 Natl. Vital Stat. Rep. 57 2009 1 134
2. C.B. Karim, Z. Zhang, E.C. Howard, K.D. Torgersen, and D.D. Thomas Phosphorylation-dependent conformational switch in spin-labeled phospholamban bound to SERCA J. Mol. Biol. 358 2006 1032 1040
3. D.M. Bers Cardiac excitation-contraction coupling Nature 415 2002 198 205
4. 2+-dependent ATPase of cardiac sarcoplasmic reticulum by adenosine 3′:5′-monophosphate-dependent protein kinase: Role of the 22,000-dalton protein J. Biol. Chem. 254 1979 319 326
5. 2+-ATPase/phospholamban in cardiomyocyte function J. Card. Fail. 2 1996 S77 S85
6. 2+-dependent ATPase of cardiac sarcoplasmic reticulum Adv. Myocardiol. 1 1980 161 171
7. 2+-dependent ATPase and calcium transport by cardiac sarcoplasmic reticulum: Effect of cyclic AMP-dependent protein kinase-catalyzed phosphorylation of phospholamban J. Biol. Chem. 255 1980 1985 1992
8. D.J. Beuckelmann, M. Nabauer, and E. Erdmann Intracellular calcium handling in isolated ventricular myocytes from patients with terminal heart failure Circulation 85 1992 1046 1055
9. 2+ sensitivity of SERCA 2a in failing human myocardium due to reduced serin-16 phospholamban phosphorylation J. Mol. Cell. Cardiol. 31 1999 479 491
10. D.H. MacLennan, and E.G. Kranias Phospholamban: a crucial regulator of cardiac contractility Nat. Rev. 4 2003 666 678
11. 2+-ATPase in patients with advanced heart failure Circulation 124 2011 304 313
12. K.R. Chien, J. Ross Jr., and M. Hoshijima Calcium and heart failure: the cycle game Nat. Med. 9 2003 508 509
13. H.K. Simmerman, J.H. Collins, J.L. Theibert, A.D. Wegener, and L.R. Jones Sequence analysis of phospholamban: identification of phosphorylation sites and two major structural domains J. Biol. Chem. 261 1986 13333 13341
14. H.K. Simmerman, and L.R. Jones Phospholamban: protein structure, mechanism of action, and role in cardiac function Physiol. Rev. 78 1998 921 947
15. P.M. Heerdt, P. Lane, B.Y. Pan, U. Schaefer, M. Crabtree, R. Hong, A.A. Singer, R. Levi, and B.J. Park Nitrosative stress and myocardial sarcoplasmic endoreticular calcium adenosine triphosphatase subtype 2a activity after lung resection in swine Anesthesiology 107 2007 954 962
16. E.J. Mayer, W. Huckle, R.G. Johnson Jr., and E. McKenna Characterization and quantitation of phospholamban and its phosphorylation state using antibodies Biochem. Biophys. Res. Commun. 267 2000 40 48
17. A. Mattiazzi, C. Mundina-Weilenmann, C. Guoxiang, L. Vittone, and E. Kranias Role of phospholamban phosphorylation on Thr17 in cardiac physiological and pathological conditions Cardiovasc. Res. 68 2005 366 375
18. 2+, and contraction following β-adrenergic stimulation of rat heart Pflugers Arch. Eur. J. Physiol. 436 1998 948 956
19. i protein, phosphodiesterase, and phosphatases Circ. Res. 85 1999 848 855
20. K. Oxenoid, A.J. Rice, and J.J. Chou Comparing the structure and dynamics of phospholamban pentamer in its unphosphorylated and pseudo-phosphorylated states Protein Sci. 16 2007 1977 1983
21. M. Hoshijima, Y. Ikeda, Y. Iwanaga, S. Minamisawa, M.O. Date, Y. Gu, M. Iwatate, M. Li, L. Wang, J.M. Wilson, Y. Wang, J. Ross Jr., and K.R. Chien Chronic suppression of heart-failure progression by a pseudophosphorylated mutant of phospholamban via in vivo cardiac rAAV gene delivery Nat. Med. 8 2002 864 871
22. 2+-ATPase by tuning phospholamban structural dynamics J. Biol. Chem. 282 2007 37205 37214
23. E.L. Lockamy, R.L. Cornea, C.B. Karim, and D.D. Thomas Functional and physical competition between phospholamban and its mutants provides insight into the molecular mechanism of gene therapy for heart failure Biochem. Biophys. Res. Commun. 408 2011 388 392
24. P. Nicolaou, R.J. Hajjar, and E.G. Kranias Role of protein phosphatase-1 inhibitor-1 in cardiac physiology and pathophysiology J. Mol. Cell. Cardiol. 47 2009 365 371
25. E.G. Kranias, and D.M. Bers Calcium and cardiomyopathies Subcell. Biochem. 45 2007 523 537
26. K.N. Ha, L.R. Masterson, Z. Hou, R. Verardi, N. Walsh, G. Veglia, and S.L. Robia Lethal Arg9Cys phospholamban mutation hinders Ca2+-ATPase regulation and phosphorylation by protein kinase A Proc. Natl. Acad. Sci. USA 108 2011 2735 2740
27. J. Colyer, and J.H. Wang Dependence of cardiac sarcoplasmic reticulum calcium pump activity on the phosphorylation status of phospholamban J. Biol. Chem. 266 1991 17486 17493
28. R.L. Cornea, L.R. Jones, J.M. Autry, and D.D. Thomas Mutation and phosphorylation change the oligomeric structure of phospholamban in lipid bilayers Biochemistry 36 1997 2960 2967
29. 2+ ATPase (SERCA2a) activity and impairs cardiac function in mice Proc. Natl. Acad. Sci. USA 101 2004 9199 9204
30. E.J. Mayer, G.M. Savage, and R.G. Johnson Jr. A quantitative immunoassay for the measurement of phospholamban levels and phosphorylation states: measurement of phospholamban levels in transgenic mouse hearts Ann. NY Acad. Sci. 853 1998 284 287
31. S.L. Robia, N.C. Flohr, and D.D. Thomas Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy Biochemistry 44 2005 4302 4311
32. S. Huke, and M. Periasamy Phosphorylation status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies J. Mol. Cell. Cardiol. 37 2004 795 799
33. 2+ signalling and muscle disease Eur. J. Biochem. 267 2000 5291 5297
34. S. Huke, and D.M. Bers Temporal dissociation of frequency-dependent acceleration of relaxation and protein phosphorylation by CaMKII J. Mol. Cell. Cardiol. 42 2007 590 599
35. A. Gorbe, Z. Giricz, A. Szunyog, T. Csont, D.S. Burley, G.F. Baxter, and P. Ferdinandy Role of cGMP-PKG signaling in the protection of neonatal rat cardiac myocytes subjected to simulated ischemia/reoxygenation Basic Res. Cardiol 105 2010 643 650
36. D.A. Ferrington, Q. Yao, T.C. Squier, and D.J. Bigelow Comparable levels of Ca-ATPase inhibition by phospholamban in slow-twitch skeletal and cardiac sarcoplasmic reticulum Biochemistry 41 2002 13289 13296
37. C.B. Karim, C.G. Marquardt, J.D. Stamm, G. Barany, and D.D. Thomas Synthetic null-cysteine phospholamban analogue and the corresponding transmembrane domain inhibit the Ca-ATPase Biochemistry 39 2000 10892 10897
38. C.B. Karim, T.L. Kirby, Z. Zhang, Y. Nesmelov, and D.D. Thomas Phospholamban structural dynamics in lipid bilayers probed by a spin label rigidly coupled to the peptide backbone Proc. Natl. Acad. Sci. USA 101 2004 14437 14442
39. C.B. Karim, M.G. Paterlini, L.G. Reddy, G.W. Hunter, G. Barany, and D.D. Thomas Role of cysteine residues in structural stability and function of a transmembrane helix bundle J. Biol. Chem. 276 2001 38814 38819
40. N.A. Lockwood, R.S. Tu, Z. Zhang, M.V. Tirrell, D.D. Thomas, and C.B. Karim Structure and function of integral membrane protein domains resolved by peptide-amphiphiles: application to phospholamban Biopolymers 69 2003 283 292
41. E.J. Mayer, E. McKenna, V.M. Garsky, C.J. Burke, H. Mach, C.R. Middaugh, M. Sardana, J.S. Smith, and R.G. Johnson Jr. Biochemical and biophysical comparison of native and chemically synthesized phospholamban and a monomeric phospholamban analog J. Biol. Chem. 271 1996 1669 1677
42. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 4350 4354
43. N.A. Ablorh, F. Nitu, K. Engebretsen, D.D. Thomas, and J.S. Holger Insulin-dependent rescue from cardiogenic shock is not mediated by phospholamban phosphorylation Clin. Toxicol. 47 2009 296 302
44. M. Li, R.L. Cornea, J.M. Autry, L.R. Jones, and D.D. Thomas Phosphorylation-induced structural change in phospholamban and its mutants, detected by intrinsic fluorescence Biochemistry 37 1998 7869 7877
45. S. Calaghan, L. Kozera, and E. White Compartmentalisation of cAMP-dependent signalling by caveolae in the adult cardiac myocyte J. Mol. Cell. Cardiol. 45 2008 88 92
46. S. Calaghan, E. White, and J. Colyer Preservation of the in vivo phosphorylation status of phospholamban in the heart: Evidence for a site-specific difference in the dephosphorylation of phospholamban Biochem. Biophys. Res. Commun. 248 1998 701 705
47. S. Grote-Wessels, H.A. Baba, P. Boknik, A. El-Armouche, L. Fabritz, H.J. Gillmann, D. Kucerova, M. Matus, F.U. Muller, J. Neumann, M. Schmitz, F. Stumpel, G. Theilmeier, J. Wohlschlaeger, W. Schmitz, and U. Kirchhefer Inhibition of protein phosphatase 1 by inhibitor-2 exacerbates progression of cardiac failure in a model with pressure overload Cardiovasc. Res. 79 2008 464 471
48. 2+ homeostasis differentially determine the progression of cTnT-related cardiomyopathies in murine models Am. J. Physiol. Heart Circ. Physiol. 297 2009 H614 H626
49. 2+-pump stimulation Biochem. J. 316 1996 201 207
50. X. Loyer, A.M. Gomez, P. Milliez, M. Fernandez-Velasco, P. Vangheluwe, L. Vinet, D. Charue, E. Vaudin, W. Zhang, Y. Sainte-Marie, E. Robidel, I. Marty, B. Mayer, F. Jaisser, J.J. Mercadier, S. Richard, A.M. Shah, J.P. Benitah, J.L. Samuel, and C. Heymes Cardiomyocyte overexpression of neuronal nitric oxide synthase delays transition toward heart failure in response to pressure overload by preserving calcium cycling Circulation 117 2008 3187 3198
51. L. Vittone, C. Mundina-Weilenmann, M. Said, and A. Mattiazzi Mechanisms involved in the acidosis enhancement of the isoproterenol-induced phosphorylation of phospholamban in the intact heart J. Biol. Chem. 273 1998 9804 9811
52. W. Luo, G. Chu, Y. Sato, Z. Zhou, V.J. Kadambi, and E.G. Kranias Transgenic approaches to define the functional role of dual site phospholamban phosphorylation J. Biol. Chem. 273 1998 4734 4739
53. D.M. Kaye, A. Preovolos, T. Marshall, M. Byrne, M. Hoshijima, R. Hajjar, J.A. Mariani, S. Pepe, K.R. Chien, and J.M. Power Percutaneous cardiac recirculation-mediated gene transfer of an inhibitory phospholamban peptide reverses advanced heart failure in large animals J. Am. Coll. Cardiol. 50 2007 253 260
54. L.T. Cooper, K.L. Baughman, A.M. Feldman, A. Frustaci, M. Jessup, U. Kuhl, G.N. Levine, J. Narula, R.C. Starling, J. Towbin, and R. Virmani The role of endomyocardial biopsy in the management of cardiovascular disease: a scientific statement from the American Heart Association, the American College of Cardiology, and the European Society of Cardiology J. Am. Coll. Cardiol. 50 2007 1914 1931