In vitro angiogenesis: Endothelial cell tube formation on gelled basement membrane extract

Nature Protocols

Volumn 5, Issue 4, 2010, Pages 628-635

  Arnaoutova, Irina ^a   Kleinman, Hynda K ^b  

^a TREVIGEN INC   (United States)

^b NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOGENESIS INHIBITOR; ANGIOGENIC FACTOR; PROTEINASE;

ANGIOGENESIS; ARTICLE; BASEMENT MEMBRANE; CAPILLARY; CELL ADHESION; CELL ASSAY; CELL MIGRATION; CELL TRANSFORMATION; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; GENE IDENTIFICATION; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; SIGNAL TRANSDUCTION; STEM CELL;

BASEMENT MEMBRANE; CAPILLARIES; CELLS, CULTURED; CYTOLOGICAL TECHNIQUES; ENDOTHELIAL CELLS; GELS; HUMANS; NEOVASCULARIZATION, PHYSIOLOGIC;

EID: 77949439845     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.6     Document Type: Article

References (33)

1. Kubota, Y., Kleinman, H.K., Martin, G.R. & Lawley, T.J. Role of laminin and basement membrane in the differentiation of human endothelial cells into capillary-like structures. J. Cell Biol. 107, 1589-1597 (1988
2. Benelli, R. & Albini, A. In vitro models of angiogenesis: the use of Matrigel. Int. J. Biol. Markers 14, 243-246 (1999
3. Auerbach, R., Lewis, R., Shinners, B., Kribai, L. & Akhtar, N. Angiogenesis assays: a critical overview. Clin. Chem. 49, 32-40 (2003
4. Arnaoutova, I., George, J., Kleinman, H.K. & Benton, G. The endothelial cell tube formation assay on basement membrane turns 20. Angiogenesis 12, 267-274 (2009
5. Kinsella, J.L., Grant, D.S., Weeks, B.S. & Kleinman, H.K. Protein kinase C regulates endothelial cell tube formation on basement membrane matrix, Matrigel. Exp. Cell Res. 199, 56-62 (1992
6. Grove, A.D. et al. Both protein activation and gene expression are involved in early vascular tube formation in vitro. Clin. Cancer Res. 8, 3019-3026 (2002
7. Grant, D.S. et al. Matrigel induces thymosin beta4 gene in differentiating endothelial cells. J. Cell Sci. 108, 3685-3694 (1995
8. Fukushima, K. et al. Gene expression profiles by microarray analysis during matrigel-induced tube formation in a human extravillous trophoblast cell line: comparison with endothelial cells. Placenta 29, 898-904 (2008
9. Bagley, R.G. et al. Endothelial precursor cells as a model of tumor endothelium: characterization and comparison with mature endothelial cells. Cancer Res. 63, 5866-5873 (2003
10. Mukai, N. et al. A comparison of the tube forming protentials of early and late endothelial progenitor cells. Exp. Cell Res. 314, 430-440 (2008
11. Rogers, M.S., Birsner, A.E. & D'amato, R.J. The mouse corneal micropocket angiogenesis assay. Nat. Protoc. 2, 2524-2550 (2007
12. Ribatti, D., Nico, B., Vacca, A. & Presta, M. The gelatin-sponge chorioallantoic membrane assay. Nat. Protoc. 1, 85-91 (2006
13. Cid, M.C. et al. Identification of haptoglobin as an angiogenic factor in sera from patients with systemic vasculitis. J. Clin. Invest. 91, 977-985 (1993
14. Grant, D.S. et al. Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures. Cell 58, 933-943 (1989
15. Kleinman, H.K. & Martin, G.R. Matrigel: Basement membrane extracellular matrix with biological activity. Semin. Cancer Biol. 15, 378-386 (2005
16. Lee, G.Y., Kenny, P.A., Lee, E.H. & Bissell, M.J. Three-dimentional culture models on normal and malignant breast epithelial cells. Nat. Methods 4, 359-365 (2007
17. Albini, A. & Benelli, R. The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation. Nat. Protoc. 2, 504-511 (2007
18. O'Connell, K.A. & Edidin, M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144, 521-525 (1990
19. Ades, E.W. et al. HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J. Invest. Dermatol. 99, 683-690 (1992
20. Shen, J.S., Meng, X.L., Schiffmann, R., Brady, R.O. & Kaneski, C.R. Establishement and characterization of Fabry disease endothelial cells with an extended lifespan. Mol. Genet. Metab. 92, 137-144 (2007
21. Van Beijnum, J.R., Rousch, M., Castermans, K., van der Linden, E. & Griffioen, A.W. Isolation of endothelial cells from fresh tissues. Nat. Protoc. 3, 1085-1091 (2008
22. Plendl, J., Neumuller, C., Vollmar, A., Auerbach, R. & Sinowatz, F. Isolation and characterization of endothelial cells from different organs of fetal pigs. Anat. Embryol. 194, 445-456 (1996
23. Yu, D. & Auerbach, R. Brain-specific differentiation of mouse yolk sac endothelial cells Brain Res. Dev. Brain Res. 117, 59-169 (1999
24. Gumkowski, F., Kaminska, G., Kaminski, M., Morrissey, L.W. & Auerbach, R. Heterogeneity of mouse vascular endothelium. In vitro studies of lymphatic, large, blood vessel and microvascular endothelial cells. Blood Vessels 24, 11-23 (1987
25. Grant, D.S., Lelkes, P.I., Fukuda, K. & Kleinman, H.K. Intracellular mechanisms involved in basement membrane induced blood vessel differentiation in vitro. In Vitro Cell Dev. Biol. 27, 327-335 (1991
26. Elkin, M. et al. Halofuginone: a potent inhibitor of critical steps in angiogenesis progression. FASEB J. 14, 2477-2485 (2000
27. Haralabopoulos, G.C. et al. Inhibitors of basement membrane collagen synthesis prevent endothelial cell alignment in Matrigel in vitro and angiogenesis in vivo. Lab. Invest. 71, 575-582 (1994
28. Rundhaug, J.E. Matrix metalloproteinases and angiogenesis. J. Cell. Mol. Med. 9, 267-285 (2007
29. Taub, M., Wang, Y., Szcesney, T.M., Kleinman, H.K. & Martin, G.R. Epidermal growth factor or transforming growth factor ? is required for kidney tubulogenesis in matrigel cultures in serum-free medium. Proc. Natl Acad. Sci. USA 87, 4002-4006 (1990
30. Vukicevic, S. et al. Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. Exp. Cell. Res 202, 1-8 (1992
31. Morales, D. et al. Estrogen promotes angiogenic activity in human umbilical vein endothelial cells in vitro and in a murine model. Circulation 91, 755-763 (1995
32. Gho, Y.S., Kleinman, H.K. & Sosne, G. Angiogenic activity of human soluble intercellular adhesion molecule-1. Cancer Res. 59, 5128-5132 (1999
33. Foubert, P. et al. Coadministration of endothelial and smooth muscle progenitor cells enhances the efficacy of proangiogenic cell-based therapy. Circ. Res. 103, 751-760 (2008