Combining label-free technologies: Discovery in strength

Bioanalysis

Volumn 4, Issue 6, 2012, Pages 619-622

  Barbour, Robin ^b   Bova, Michael P ^a  

^a ELAN PHARMACEUTICALS   (United States)

^b NONE

Author keywords

biolayer interferometry; biomolecular screening; differential scanning calorimetry; drug discovery; isothermal calorimetry; label free technology; plate based optical biosensors; quartz crystal microbalance; surface plasmon resonance

Indexed keywords

G PROTEIN COUPLED RECEPTOR; RECOMBINANT PROTEIN;

ANTIBODY AFFINITY; BIOTECHNOLOGY; BIOTINYLATION; CELL ASSAY; DIFFERENTIAL SCANNING CALORIMETRY; DRUG DEVELOPMENT; EDITORIAL; HIGH THROUGHPUT SCREENING; INTERFEROMETRY; OPTICAL BIOSENSOR; OPTICAL INSTRUMENTATION; PROTEIN BINDING; PROTEIN TARGETING; QUARTZ CRYSTAL MICROBALANCE; SURFACE PLASMON RESONANCE;

BIOSENSING TECHNIQUES; CALORIMETRY, DIFFERENTIAL SCANNING; DRUG DISCOVERY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INTERFEROMETRY; QUARTZ CRYSTAL MICROBALANCE TECHNIQUES; RECEPTORS, G-PROTEIN-COUPLED; SURFACE PLASMON RESONANCE;

EID: 84859153398     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.12.45     Document Type: Editorial

References (18)

1. Cunningham BT, Li P, Schulz S et al. Label-free assays on the BIND system. J. Biomol. Screen. 9(6), 481-490 (2004).
2. Barbour R. Combining label free assay platforms to support both large and small molecule drug discovery. Presented at: Screening Summit-World Pharmaceutical Congress. Philadelphia, PA, USA, 8 June 2011.
3. Bova MP, Nguyen L, Wallace W et al. A label-free approach to identify inhibitors of a4b7-mediated cell adhesion to MadCAM. J. Biomol. Screen. 16(5), 536-544 (2011).
4. McGuinness RP, Proctor JM, Gallant DL et al. Enhanced selectivity screening of GPCR ligands using a label-free cell based assay technology. Comb. Chem. High Throughput Screen. 12(8), 812-823 (2009).
5. Concepcion J, Witte K, Wartchow C et al. Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization. Comb. Chem. High Throughput Screen. 12(8), 791-800 (2009).
6. Wartchow CA, Podlaski F, Li S et al. Biosensor-based small molecule fragment screening with biolayer interferometry. J. Comput. Aided Mol. Des. 25(7), 669-676 (2011).
7. Do T, Ho F, Heidecker B, Witte K, Chang L, Lerner L. A rapid method for determining dynamic binding capacity of resins for the purification of proteins. Protein Exp. Purif. 60(2), 147-150 (2008).
8. Abdiche YN, Malashock DS, Pinkerton A, Pons J. Exploring blocking assays using Octet, ProteOn, and Biacore biosensors. Anal. Biochem. 386(2), 172-180 (2009).
9. Abdiche Y, Malashock D, Pinkerton A, Pons J. Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet. Anal. Biochem. 377(2), 209-217 (2008).
10. Abdiche YN, Lindquist KC, Pinkerton A, Pons J, Rajpal A. Expanding the ProteOn XPR36 biosensor into a 36-ligand array expedites protein interaction analysis. Anal. Biochem. 411(1), 139-151 (2011).
11. Jason-Moller L, Murphy M, Bruno J. Overview of Biacore systems and their applications. Curr. Protoc. Protein. Sci. 19, 19.13 (2006).
12. Löfås S. Optimizing the hit-to-lead process using SPR analysis. Assay. Drug. Dev. Technol. 2(4), 407-415 (2004).
13. Säfsten P, Klakamp SL, Drake AW, Karlsson R, Myszka DG. Screening antibody-antigen interactions in parallel using Biacore A100. Anal. Biochem. 353(2), 181-190 (2006).
14. Stumbaum M, Burkhard C, Gronewold T et al. Binding kinetics of therapeutic antibodies to native cell surgace proteins on living cells. Presented at: Miptec. Basel, Switzerland, 22 September 2011.
15. Pei Z, Elovsson K, Käck C, Kaiser L, Aastrup T. Label free cell based biosensors: bridging the gap between biosensors and cell based assays. Presented at: Next Generation Pharma Summit. Scottsdale, AZ, USA, 26 May 2011.
16. Chaires JB. Calorimetry and thermodynamics in drug design. Annu. Rev. Biophys. 37, 135-151 (2008).
17. Ahrer K, Buchacher A, Iberer G, Jungbauer A. Thermodynamic stability and formation of aggregates of human immunoglobulin G characterised by differential scanning calorimetry and dynamic light scattering. J. Biochem. Biophys. Methods 66(1-3), 73-86 (2006).
18. Rich RL, Myszka DG. Survey of the 2009 commercial optical biosensor literature. J. Mol. Recognit. 24(6), 892-914 (2011).