A rapid method for determining dynamic binding capacity of resins for the purification of proteins

Protein Expression and Purification

Volumn 60, Issue 2, 2008, Pages 147-150

  Do, Tri ^a   Ho, Frank ^a   Heidecker, Bettina ^b   Witte, Krista ^b   Chang, Lucille ^a   Lerner, Laura ^a  

^a Raven Biotechnologies Inc   (United States)

^b ForteBio Inc   (United States)

Author keywords

Biolayer interferometry; Chromatography; Dynamic binding capacity; Light diffraction; Protein assay; Protein purification

Indexed keywords

PROTEIN;

ANIMAL; ARTICLE; CHO CELL; CRICETULUS; ENZYME LINKED IMMUNOSORBENT ASSAY; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING;

ANIMALS; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; PROTEIN BINDING; PROTEINS;

EID: 45449104608     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.04.009     Document Type: Article

References (8)

1. McCue J.T., Kemp G., Low D., and Quinones-Garcia I. Evaluation of protein-A chromatography media. J. Chromatogr. A 989 (2003) 139-153
2. Hahn R., Bauerhansl P., Shimahara K., Wizniewski C., Tscheliessnig A., and Jungbauer A. Comparison of protein A affinity sorbents II. Mass transfer properties. Chromatogr. A 1093 (2005) 98-110
3. Brorson K., Brown J., Hamilton E., and Stein K.E. Identification of protein A media performance attributes that can be monitored as surrogates for retrovirus clearance during extended re-use. J. Chromatogr. A 989 (2003) 155-163
4. Hahn R., Shimahara K., Steindl F., and Jungabauer A. Comparison of protein A affinity sorbents III. Life time study. J. Chromatogr. A 1102 (2006) 224-231
5. Hahn R., Schlegel R., and Jungbauer A. Comparison of protein A affinity sorbents. J. Chromatogr. B 790 (2003) 35-51
6. Rich R.L., and Myszka D.G. Higher-throughput, label-free, real-time molecular interaction analysis. Anal. Biochem. 361 (2007) 1-6
7. Rich R.L., and Myszka D.G. Survey of the year 2006 commercial optical biosensor literature. J. Mol. Recognit. 20 (2007) 300-366
8. In: Taylor R.F. (Ed). Protein Immobilization: Fundamentals and Applications (1991), Marcel Dekker Inc.