Fluorogenic substrates with single fluorophores for nucleic acid-modifying enzymes: Design principles and new applications

Analytical Biochemistry

Volumn 424, Issue 2, 2012, Pages 142-148

  Nikiforov, Theo T ^a  

^a Life Technologies Corporation   (United States)

Author keywords

DNA repair enzymes; Enzymatic assays; Exonucleases; Fluorescence; Fluorescence quenching; Fluorophore stacking; FPG; T4 polynucleotide kinase; Taq polymerase; UDG

Indexed keywords

AROMATIC COMPOUNDS; DNA; ENZYMES; FLUORESCENCE; FLUORESCENCE QUENCHING; OLIGONUCLEOTIDES; QUENCHING; SYNTHESIS (CHEMICAL);

DNA-REPAIR ENZYMES; ENZYMATIC ASSAY; EXONUCLEASES; T4 POLYNUCLEOTIDE KINASE; TAQ POLYMERASE;

FLUOROPHORES;

DNA FORMAMIDOPYRIMIDINE GLYCOSYLASE; DNA POLYMERASE; ENDONUCLEASE; ENZYME; LIGASE; NUCLEIC ACID MODIFYING ENZYME; OLIGONUCLEOTIDE; PHOSPHODIESTERASE I; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; SPLEEN EXONUCLEASE; TAQ POLYMERASE; UNCLASSIFIED DRUG; URACIL DNA GLYCOSIDASE;

ARTICLE; DNA HAIRPIN; DNA REPAIR; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; FLUORESCENCE; NONPHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL;

EID: 84859067819     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.031     Document Type: Article

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