Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system

Biotechnology and Bioengineering

Volumn 55, Issue 6, 1997, Pages 854-863

  Hasenwinkle, Diane ^a   Jervis, Eric ^a   Kops, Oliver ^a   Liu, Chang ^a   Lesnicki, Gary ^b   Haynes, Charles A ^a   Kilburn, Douglas G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b NONE

Author keywords

Affinity purification; Cellulose binding protein; High density fermentations; Recombinant protein production

Indexed keywords

BATCH CELL CULTURE; BIOSYNTHESIS; FERMENTATION; GENES; GENETIC ENGINEERING; POLYPEPTIDES; PROTEINS; PURIFICATION; SOLUTIONS;

AFFINITY PURIFICATION; CELLULOSE BINDING PROTEIN; RECOMBINANT PROTEIN PRODUCTION;

COLIFORM BACTERIA;

BINDING PROTEIN; CELLULOSE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BINDING AFFINITY; CULTURE MEDIUM; ESCHERICHIA COLI; FERMENTATION; GENETIC RECOMBINATION; GROWTH RATE; NONHUMAN; PROTEIN PURIFICATION;

EID: 0342276083     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970920)55:6<854::AID-BIT4>3.0.CO;2-F     Document Type: Article

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