Development of a high-throughput screening method for LIM kinase 1 using a luciferase-based assay of ATP consumption

Journal of Biomolecular Screening

Volumn 17, Issue 4, 2012, Pages 460-468

  Mezna, Mokdad ^a   Wong, Ai Ching ^b   Ainger, Margaret ^b   Scott, Rebecca W ^a   Hammonds, Tim ^b   Olson, Michael F ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

assay; cancer and cancer drugs; enzyme assay; high throughput screen; LIM kinase; luminescence

Indexed keywords

ADENOSINE TRIPHOSPHATE; ENZYME INHIBITOR; LIM KINASE 1; LUCIFERASE;

ACCURACY; ARTICLE; CONTROLLED STUDY; ENERGY CONSUMPTION; ENZYME ACTIVITY; ENZYME ASSAY; HIGH THROUGHPUT SCREENING; HUMAN; LUMINESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; RELIABILITY;

ACTIN DEPOLYMERIZING FACTORS; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; DRUG EVALUATION, PRECLINICAL; ENZYME ACTIVATION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LIM KINASES; LUCIFERASES; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS; SMALL MOLECULE LIBRARIES;

EID: 84858953576     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111430529     Document Type: Article

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