A rapid method for determining protein kinase phosphorylation specificity

Nature Methods

Volumn 1, Issue 1, 2004, Pages 27-29

  Hutti, Jessica E ^a   Jarrell, Emily T ^a   Chang, James D ^a   Abbott, Derek W ^a   Storz, Peter ^a   Toker, Alex ^a   Cantley, Lewis C ^a   Turk, Benjamin E ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PEPTIDE LIBRARY; PROTEIN SERINE THREONINE KINASE;

ARTICLE; BINDING SITE; CHEMISTRY; ENZYME ACTIVATION; ENZYME SPECIFICITY; EVALUATION; METABOLISM; METHODOLOGY; MICROFLUIDIC ANALYSIS; PHOSPHORYLATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MOTIF; SEQUENCE ANALYSIS;

AMINO ACID MOTIFS; BINDING SITES; ENZYME ACTIVATION; MICROFLUIDIC ANALYTICAL TECHNIQUES; PEPTIDE LIBRARY; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN-SERINE-THREONINE KINASES; SEQUENCE ANALYSIS, PROTEIN; SUBSTRATE SPECIFICITY;

EID: 17644391197     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth708     Document Type: Article

References (15)

1. Manning, G., Whyte, D.B., Martinez, R., Hunter, T & Sudarsanam, S. Science 298, 1912-1934 (2002).
2. Pinilla, C., Appel, J.R., Blanc, P. & Houghten, R.A. Biotechniques 13, 901-905 (1992).
3. Songyang, Z. Mol. Cell. Biol. 16, 6486-6493 (1996).
4. Nishikawa, K., Toker, A., Johannes, F.J., Songyang, Z. & Cantley, L.C. J. Biol. Chem. 272, 952-960 (1997).
5. Yaffe, M.B. et al. Nat. Biotechnol. 19, 348-353 (2001).
6. Meggio, F. & Pinna, L.A. FASEB J. 17, 349-368 (2003).
7. Wu, J.J., Phan, H. & Lam, K.S. Bioorg. Med. Chem Lett. 8, 2279-2284 (1998).
8. Rychlewski, L., Kschischo, M., Dong, L., Schutkowski, M. & Reimer, U. J. Mol. Biol. 336, 307-311 (2004).
9. Rodriguez, M., Li, S.S., Harper, J.W. & Songyang, Z. J. Biol. Chem. 279, 8802-8807 (2004).
10. Luo, K., Zhou, P. & Lodish, H.F. Proc. Natl. Acad. Sci. USA 92, 11761-11765 (1995).
11. Massagué, J. Ann. Rev. Biochem. 67, 753-791 (1998).
12. Mikkers, H. et al. Nat. Genet. 32, 153-159 (2002).
13. Friedmann, M., Nissen, M.S., Hoover, D.S., Reeves, R. & Magnuson, N.S. Arch. Biochem Biophys. 298, 594-601 (1992).
14. Yan, B. et al. J. Biol. Chem. 278, 45358-45367 (2003).
15. Fox, C.J. et al. Genes Dev. 17, 1841-1854 (2003).