High-throughput determination of mode of inhibition in lead identification and optimization

Journal of Biomolecular Screening

Volumn 12, Issue 2, 2007, Pages 220-228

  Wei, Min ^a   Wynn, Richard ^b   Hollis, Gregory ^b   Liao, Boshan ^b   Margulis, Alexander ^b   Reid, Brian G ^c   Klabe, Ronald ^b   Liu, Phillip C C ^b   Becker Pasha, Mary ^b   Rupar, Mark ^b   Burn, Timothy C ^b   McCall, Dale E ^b   Li, Yanlong ^b  

^a GLAXOSMITHKLINE   (United States)

^b Experimental Station   (United States)

^c Myogen Inc   (United States)

Author keywords

Competitive; High throughput screening; Leads identification and optimization; Mode of inhibition; Noncompetitive

Indexed keywords

ENZYME; G PROTEIN COUPLED RECEPTOR; LIGAND; PROTEIN TYROSINE PHOSPHATASE 1B;

ARTICLE; CHEMICAL ANALYSIS; CONCENTRATION (PARAMETERS); ENZYME ASSAY; ENZYME KINETICS; EVALUATION; HIGH THROUGHPUT SCREENING; HUMAN; IC 50; MOLECULAR DYNAMICS; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN TARGETING; THEORY;

ADENOSINE TRIPHOSPHATE; ANIMALS; BACULOVIRIDAE; BINDING SITES; CATALYTIC DOMAIN; CELL LINE; COMBINATORIAL CHEMISTRY TECHNIQUES; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; ESCHERICHIA COLI; HISTIDINE; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; LIGANDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-TYROSINE-PHOSPHATASE; RECEPTORS, G-PROTEIN-COUPLED; SPODOPTERA;

EID: 33847622408     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057106296679     Document Type: Article

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