N-terminal purification tag alters thermal stability of the carboxylesterase EstGtA2 from G. thermodenitrificans by impairing reversibility of thermal unfolding

Protein and Peptide Letters

Volumn 19, Issue 3, 2012, Pages 264-269

  Charbonneau, David M ^a,b   Meddeb Mouelhi, Fatma ^a   Beauregard, Marc ^a,b  

^a UNIVERSITÉ DU QUÉBEC À TROIS RIVIÈRES   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Carboxylesterase; Circular dichroism; Geobacillus thermodenitrificans; Tag peptide; Thermal stability; Thermal unfolding

Indexed keywords

BACTERIAL ENZYME; CARBOXYLESTERASE; POLYHISTIDINE TAG;

AMINO TERMINAL SEQUENCE; ARTICLE; CIRCULAR DICHROISM; COLORIMETRY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVITY; GEOBACILLUS; GEOBACILLUS THERMODENITRIFICANS; MELTING POINT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN REFOLDING; PROTEIN STABILITY; PROTEIN UNFOLDING; ROOM TEMPERATURE; SEQUENCE TAGGED SITE; TEMPERATURE MEASUREMENT; THERMOSTABILITY;

ARTIFACTS; CARBOXYLESTERASE; ENZYME STABILITY; GEOBACILLUS; PROTEIN CONFORMATION; PROTEIN REFOLDING; PROTEIN UNFOLDING; RECOMBINANT FUSION PROTEINS; TEMPERATURE; THERMODYNAMICS;

GEOBACILLUS THERMODENITRIFICANS;

EID: 84858855137     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986612799363181     Document Type: Article

References (19)

1. Terpe, K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbio. Biotechnol., 2003, 60, 523-533. (Pubitemid 36169526)
2. Hedhammar, M.; Gräslunf, T.; Uhlén, M.; Hober, S. Negatively charged purification tags for selective anion-exchange recovery. Prot. Eng. Des. Sel., 2004, 17, 779-786. (Pubitemid 40313671)
3. Carson, M.; Johnson, D.H.; McDonald, H.; Brouilette, C.; Lawrence, J.D. His-tag impact on structure. Acta Cryst D., 2007, D63, 295-301. (Pubitemid 46438522)
4. Derewenda, Z.S. The use of recombinant methods and molecular engineering in protein crystallization. Methods., 2004, 34, 354-363. (Pubitemid 39119819)
5. Woestenenk, E.A.; Hammarström, M.; van den Berg, S.; Härd, T.; Berglund, H. His-tag effect on solubility of human proteins produced in Escherichia coli: a comparison between fuor expression vectors. J. Struct. Funct. Genomics., 2004, 5, 217-229. (Pubitemid 39030613)
6. Amor-Mahjoub, M.; Suppini, J.P.; Gomez-Vrielyunck, N.; Ladjimi, M. The effect of the hexahistidine-tag in the oligomerization of HSC70 construct. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 2006, 844, 328-334. (Pubitemid 44792013)
7. Lee, Y-N.; Su, M-S.; Huang, T-H.; Shaw, J-F. C-terminal Histagging results in substarte specificity changes of the thioesterase I from Escherichia coli. J. Am. Oil. Chem. Soc., 1999, 76, 1113-1118. (Pubitemid 30528814)
8. Sayari, A.; Mosbah, H.; Verger, R.; Gargouri, Y. The N-terminal His-tag affects the enantionselectivity of staphylococcal lipases: A monolayer study. J. Coll. Int. Sci., 2007, 313, 261-267. (Pubitemid 47042800)
9. Consalvi, V.; Chiaraluce, R.; Giangacomo, L.; Scandurra, R.; Christova, P.; Karshikoff, A., Knapp, S., and Ladenstein, R. Thermal unfolding and conformational stability of the recombinant domain II of glutamate dehydrogenase from the hyperthermophile Thermotoga maritima. Prot. Eng., 2000, 13(7), 501-507. (Pubitemid 30608562)
10. JunGang, L.; KeGui, Z.; and WenJun, Han. Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product. Microb. Cell Fact., 2010, 9, 83-87.
11. Bertolini, M.C.; Schrag, J.D.; Cygler, M.; Ziomek, E.; Thomas, D.Y.; Vernet, T. Expression and characterization of Geotrichum candidum lipase I gene. Eur. J. Biochem., 1995, 228, 863-869.
12. Blank, K.; Morfill, J.; Gumpp, H.; Gaub, HE. Functional expression of Candida antartica lipase B in Escherichia coli. J. Biotech., 2006, 125, 474-483. (Pubitemid 44333128)
13. Charbonneau, D.M.; Meddeb-Mouelhi, F.; Beauregard, M. A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: Evidence for a new carboxylesterase family. J. Biochem., 2010, 148, 299-308.
14. Smith, P.K.; Krohn, R.I.; Hermanson, G.T.; Mailla, A.K.; Gartner, F.H.; Provenzano, M. D.; Fujimoto, E. K.; Goeke, N. M.; Olson, B. J.; and Klenk, D. C. Measurement of protein using bicinchoninic acid. Anal. Biochem., 1985, 150, 76-85. (Pubitemid 16258399)
15. Sreerama, N.; and Woody, R.W. Estimation of the number of ahelical and b-strand segments in proteins using CD spectroscopy Protein Sci., 1999, 8, 370-380. (Pubitemid 29072437)
16. Sreerama, N.; Manning, M.C.; Powers, M.E.; Goldenberg, D.M.; and Woody, R.W. Tyrosine, phenylalanine and disulfide contribution to the circular dichroism of proteins: circular dichroism spectra of wild-type and mutant bovine pancreatic trypsin inhibitor. Biochemistry., 1999, 33, 10814-10822.
17. Sreerama, N.; and Woody, R.W. Estimation of protein secondary structure from circular dichroism spectra: Comparison on CONTIN, SELCON, and CDSSTR methods with an expended reference set. Anal. Biochem., 2000, 287, 252-260. (Pubitemid 32006234)
18. Pace, C.N.; Shirley, B.A.; Thomson, J.A. In: Creighton, TE, Ed. Protein Structure-A practical approach, IRL Press: Oxford, 1990; 311-329.
19. Wear, M.A.; Patterson, A.; and Walkinshaw, M.D. A kinetically trapped intermediate of FK506 binding protein forms in vitro: chaperone machinery dominates protein folding in vivo. Prot. Exp. Purif., 2006, 51, 80-95. (Pubitemid 44822661)