Negatively charged purification tags for selective anion-exchange recovery

Protein Engineering, Design and Selection

Volumn 17, Issue 11, 2004, Pages 779-786

  Hedhammar, M ^a   Graslund T ^a   Uhlen M ^a   Hober, S ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Ion exchange chromatography; Protein A; Protein design; Z domain

Indexed keywords

AMINO ACIDS; CELLS; CHROMATOGRAPHIC ANALYSIS; FLUORESCENCE; GENES; ION EXCHANGE; PH EFFECTS; PROTEINS; PURIFICATION; SODIUM CHLORIDE;

ANION-EXCHANGE CHROMATOGRAPHY; GREEN FLUORESCENT PROTEINS (GFP); MALTOSE BINDING PROTEINS (MBP); PH VALUES;

NEGATIVE IONS;

AMINO ACID; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LUCIFERASE; MALTOSE BINDING PROTEIN; RECOMBINANT DNA; RECOMBINANT PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

AMINO ACID SEQUENCE; CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY, ION EXCHANGE; CIRCULAR DICHROISM; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

EID: 14644444571     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh092     Document Type: Article

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