Online immunoaffinity LC/MS/MS. A general method to increase sensitivity and specificity: How do you do it and what do you need?

Methods

Volumn 56, Issue 2, 2012, Pages 236-245

  Dufield, Dawn R ^a   Radabaugh, Melissa R ^b  

^a PFIZER INC   (United States)

^b Sigma Aldrich Co   (United States)

Author keywords

Antibody enrichment; IA LC MRM; Immunoaffinity LC MS MS; Online SPE; Protein quantitation (SISCAPA); Triple quadrupole

Indexed keywords

ANTIBODY DETECTION; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOAFFINITY CHROMATOGRAPHY; LIGAND BINDING; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DEGRADATION; SENSITIVITY AND SPECIFICITY; STANDARD; TANDEM MASS SPECTROMETRY;

ANTIBODIES, IMMOBILIZED; BIOLOGICAL MARKERS; CHROMATOGRAPHY, AFFINITY; EDUCATION, DISTANCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOASSAY; INTERNET; LIGANDS; PEPTIDES; SENSITIVITY AND SPECIFICITY; SOFTWARE; TANDEM MASS SPECTROMETRY;

EID: 84858440044     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2011.08.012     Document Type: Article

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