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Volumn 32, Issue 5, 2012, Pages 899-908

Molecular cloning and characterization of nucleotide binding and oligomerization domain-1 (NOD1) receptor in the Indian Major Carp, rohu (Labeo rohita), and analysis of its inductive expression and down-stream signalling molecules following ligands exposure and Gram-negative bacterial infections

Author keywords

Indian Major Carp; Labeo rohita; NOD1; RICK

Indexed keywords

AEROMONAS HYDROPHILA; BACTERIA (MICROORGANISMS); CTENOPHARYNGODON; CYPRINIDAE; EDWARDSIELLA TARDA; LABEO ROHITA; NEGIBACTERIA; SHIGELLA FLEXNERI;

EID: 84858342796     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2012.02.018     Document Type: Article
Times cited : (62)

References (55)
  • 2
    • 32944464648 scopus 로고    scopus 로고
    • Pathogen recognition and innate immunity
    • Akira S., Uematsu S., Takeuchi O. Pathogen recognition and innate immunity. Cell 2006, 124:783-801.
    • (2006) Cell , vol.124 , pp. 783-801
    • Akira, S.1    Uematsu, S.2    Takeuchi, O.3
  • 3
    • 22844435410 scopus 로고    scopus 로고
    • Innate immunity of fish (overview)
    • Magnadottir B. Innate immunity of fish (overview). Fish Shellfish Immunol 2006, 20:137-151.
    • (2006) Fish Shellfish Immunol , vol.20 , pp. 137-151
    • Magnadottir, B.1
  • 4
    • 0030666222 scopus 로고    scopus 로고
    • Innate immunity: the virtues of a nonclonal system of recognition
    • Medzhitov R., Janeway C.A. Innate immunity: the virtues of a nonclonal system of recognition. Cell 1997, 91:295-298.
    • (1997) Cell , vol.91 , pp. 295-298
    • Medzhitov, R.1    Janeway, C.A.2
  • 5
    • 70249110860 scopus 로고    scopus 로고
    • Cutting edge: TNF-α mediated sensitization to ATP and Silica via the NLRP3 inflammasome in the absence of microbial stimulation
    • Franchi L., Eigenbrod T., Nunez G. Cutting edge: TNF-α mediated sensitization to ATP and Silica via the NLRP3 inflammasome in the absence of microbial stimulation. J Immunol 2009, 183:792-796.
    • (2009) J Immunol , vol.183 , pp. 792-796
    • Franchi, L.1    Eigenbrod, T.2    Nunez, G.3
  • 6
    • 66149086936 scopus 로고    scopus 로고
    • NOD-like receptors: role in innate immunity and inflammatory disease
    • Chen G., Shaw M.H., Kim Y.G., Nunez G. NOD-like receptors: role in innate immunity and inflammatory disease. Annu Rev Pathol Mech 2009, 4:365-398.
    • (2009) Annu Rev Pathol Mech , vol.4 , pp. 365-398
    • Chen, G.1    Shaw, M.H.2    Kim, Y.G.3    Nunez, G.4
  • 7
    • 5144228220 scopus 로고    scopus 로고
    • Toll-like receptors and Nod proteins in bacterial infection
    • Philpott D.J., Girardin S.E. Toll-like receptors and Nod proteins in bacterial infection. Mol Immunol 2004, 41:1099-1108.
    • (2004) Mol Immunol , vol.41 , pp. 1099-1108
    • Philpott, D.J.1    Girardin, S.E.2
  • 8
    • 51849103168 scopus 로고    scopus 로고
    • The microbial and danger signals that activate NOD-like receptors
    • Benko S., Philpott D.J., Girardin S.E. The microbial and danger signals that activate NOD-like receptors. Cytokine 2008, 43:368-373.
    • (2008) Cytokine , vol.43 , pp. 368-373
    • Benko, S.1    Philpott, D.J.2    Girardin, S.E.3
  • 9
    • 38149113537 scopus 로고    scopus 로고
    • NLR proteins: integral members of innate immunity and mediators of inflammatory diseases
    • Wilmanski J.M., Ocwieja T.P., Kobayashi K.S. NLR proteins: integral members of innate immunity and mediators of inflammatory diseases. J Leukoc Biol 2008, 83:13-30.
    • (2008) J Leukoc Biol , vol.83 , pp. 13-30
    • Wilmanski, J.M.1    Ocwieja, T.P.2    Kobayashi, K.S.3
  • 11
    • 72949115274 scopus 로고    scopus 로고
    • Roll of Toll-like receptors, NOD-like receptors and RIG-I-like receptors in endothelial cells and systemic infections
    • Opitz B., Eitel J., Meixenberger K., Suttorp N. Roll of Toll-like receptors, NOD-like receptors and RIG-I-like receptors in endothelial cells and systemic infections. J Thromb Haemost 2009, 102:1103-1109.
    • (2009) J Thromb Haemost , vol.102 , pp. 1103-1109
    • Opitz, B.1    Eitel, J.2    Meixenberger, K.3    Suttorp, N.4
  • 14
    • 0033591330 scopus 로고    scopus 로고
    • Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB
    • Inohara N., Koseki T., Peso L.D., Hu Y., Yee C., Chen S., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J Biol Chem 1999, 274:14560-14567.
    • (1999) J Biol Chem , vol.274 , pp. 14560-14567
    • Inohara, N.1    Koseki, T.2    Peso, L.D.3    Hu, Y.4    Yee, C.5    Chen, S.6
  • 15
    • 0033532091 scopus 로고    scopus 로고
    • Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB
    • Bertin J., Nir W.J., Fischer C.M., Tayber O.V., Errada P.R., Grant J.R., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB. J Biol Chem 1999, 274:12955-12958.
    • (1999) J Biol Chem , vol.274 , pp. 12955-12958
    • Bertin, J.1    Nir, W.J.2    Fischer, C.M.3    Tayber, O.V.4    Errada, P.R.5    Grant, J.R.6
  • 16
    • 0038615855 scopus 로고    scopus 로고
    • Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan
    • Girardin S.E., Boneca I.G., Carneiro L.A., Antignac A., Jehanno M., Viala J., et al. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science 2003, 300:1584-1587.
    • (2003) Science , vol.300 , pp. 1584-1587
    • Girardin, S.E.1    Boneca, I.G.2    Carneiro, L.A.3    Antignac, A.4    Jehanno, M.5    Viala, J.6
  • 17
    • 0038824980 scopus 로고    scopus 로고
    • An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid
    • Chamaillard M., Hashimoto M., Horie Y., Masumoto J., Qiu S., Saab L., et al. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat Immunol 2003, 4:702-707.
    • (2003) Nat Immunol , vol.4 , pp. 702-707
    • Chamaillard, M.1    Hashimoto, M.2    Horie, Y.3    Masumoto, J.4    Qiu, S.5    Saab, L.6
  • 19
    • 37749012084 scopus 로고    scopus 로고
    • NOD1 and NOD2 in innate immunity and human inflammatory disorders
    • Bourhis L.L., Benko S., Girardin S.E. NOD1 and NOD2 in innate immunity and human inflammatory disorders. Biochem Soc Trans 2007, 35:1479-1484.
    • (2007) Biochem Soc Trans , vol.35 , pp. 1479-1484
    • Bourhis, L.L.1    Benko, S.2    Girardin, S.E.3
  • 20
    • 70350356853 scopus 로고    scopus 로고
    • Activating immunity: lessons from the TLRs and NLRs
    • Monie T.P., Bryant C.E., Gay N.J. Activating immunity: lessons from the TLRs and NLRs. Trends Biochem Sci 2009, 34:553-561.
    • (2009) Trends Biochem Sci , vol.34 , pp. 553-561
    • Monie, T.P.1    Bryant, C.E.2    Gay, N.J.3
  • 22
    • 73049088885 scopus 로고    scopus 로고
    • Molecular characterization and expression analysis of nuclear oligomerisation domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella
    • Chen W.Q., Xu Q.Q., Chang M.X., Nie P., Peng K.M. Molecular characterization and expression analysis of nuclear oligomerisation domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol 2010, 28:18-29.
    • (2010) Fish Shellfish Immunol , vol.28 , pp. 18-29
    • Chen, W.Q.1    Xu, Q.Q.2    Chang, M.X.3    Nie, P.4    Peng, K.M.5
  • 23
    • 38549084725 scopus 로고    scopus 로고
    • A critical role of RICK/RIP2 polyubiquitination in Nod-induced NF-κB activation
    • Hasegawa M., Fujimoto Y., Lucas P.C., Nakano H., Fukase K., Nunez G., et al. A critical role of RICK/RIP2 polyubiquitination in Nod-induced NF-κB activation. EMBO J 2008, 27:373-383.
    • (2008) EMBO J , vol.27 , pp. 373-383
    • Hasegawa, M.1    Fujimoto, Y.2    Lucas, P.C.3    Nakano, H.4    Fukase, K.5    Nunez, G.6
  • 25
    • 0034623225 scopus 로고    scopus 로고
    • An induced proximity model for NF-kB activation in the Nod1/RICK and RIP signaling pathway
    • Inohara N., Koseki T., Lin J., Peso L., Lucas P.C., Chen F.F., et al. An induced proximity model for NF-kB activation in the Nod1/RICK and RIP signaling pathway. J Biol Chem 2000, 275:27823-27831.
    • (2000) J Biol Chem , vol.275 , pp. 27823-27831
    • Inohara, N.1    Koseki, T.2    Lin, J.3    Peso, L.4    Lucas, P.C.5    Chen, F.F.6
  • 27
    • 33644849605 scopus 로고    scopus 로고
    • NOD2/CARD 15 mediates induction of the antimicrobial peptide human beta-defensin-2
    • Voss E., Wehkamp J., Wehkamp K., Stange E.F., Schroder J.M., Harder J. NOD2/CARD 15 mediates induction of the antimicrobial peptide human beta-defensin-2. J Biol Chem 2006, 281:2005-2011.
    • (2006) J Biol Chem , vol.281 , pp. 2005-2011
    • Voss, E.1    Wehkamp, J.2    Wehkamp, K.3    Stange, E.F.4    Schroder, J.M.5    Harder, J.6
  • 29
    • 38149125275 scopus 로고    scopus 로고
    • Molecular cloning and functional characterization of porcine Nucleotide-binding oligomerization domain-1 (NOD1) recognizing minimum agonists, meso- diaminopimelic acid and meso-lanthionine
    • Tohno M., Shimazu T., Aso H., Uehara A., Takada H., Kawasaki A., et al. Molecular cloning and functional characterization of porcine Nucleotide-binding oligomerization domain-1 (NOD1) recognizing minimum agonists, meso- diaminopimelic acid and meso-lanthionine. Mol Immunol 2008, 45:1807-1817.
    • (2008) Mol Immunol , vol.45 , pp. 1807-1817
    • Tohno, M.1    Shimazu, T.2    Aso, H.3    Uehara, A.4    Takada, H.5    Kawasaki, A.6
  • 30
    • 40949159815 scopus 로고    scopus 로고
    • A genomic review of the NOD-like receptor family in teleost fish: identification of novel NLR subfamily in Zebrafish
    • Laing K.J., Purcell M.K., Winton J.R., Hansen J.D. A genomic review of the NOD-like receptor family in teleost fish: identification of novel NLR subfamily in Zebrafish. BMC Evo Biol 2008, 8:42.
    • (2008) BMC Evo Biol , vol.8 , pp. 42
    • Laing, K.J.1    Purcell, M.K.2    Winton, J.R.3    Hansen, J.D.4
  • 31
    • 81455134351 scopus 로고    scopus 로고
    • The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish
    • Oehlers S.H., Flores M.V., Hall C.J., Swift S., Crosier K.E., Crosider P.S. The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish. Dis Models Mech 2011, 10.1242/dmm.006122.
    • (2011) Dis Models Mech
    • Oehlers, S.H.1    Flores, M.V.2    Hall, C.J.3    Swift, S.4    Crosier, K.E.5    Crosider, P.S.6
  • 32
    • 67349242443 scopus 로고    scopus 로고
    • NOD-like subfamily of the nucleotide-binding domain and leucine-rich repeat containing family receptors and their expression in channel catfish
    • Sha Z., Abernathy J.W., Wang S., Li P., Kucuktas H., Liu H., et al. NOD-like subfamily of the nucleotide-binding domain and leucine-rich repeat containing family receptors and their expression in channel catfish. Dev Comp Immunol 2009, 33:991-999.
    • (2009) Dev Comp Immunol , vol.33 , pp. 991-999
    • Sha, Z.1    Abernathy, J.W.2    Wang, S.3    Li, P.4    Kucuktas, H.5    Liu, H.6
  • 33
    • 78650689216 scopus 로고    scopus 로고
    • Cloning of two rainbow trout nucleotide-binding oligomerisation domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effectors domains
    • Chang M., Wang T., Nie P., Zou J., Secombes C.J. Cloning of two rainbow trout nucleotide-binding oligomerisation domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effectors domains. Fish Shellfish Immunol 2011, 30:118-127.
    • (2011) Fish Shellfish Immunol , vol.30 , pp. 118-127
    • Chang, M.1    Wang, T.2    Nie, P.3    Zou, J.4    Secombes, C.J.5
  • 34
    • 79959790384 scopus 로고    scopus 로고
    • Molecular characterization, expression and functional analysis of a nuclear oligomerization domain proteins subfamily C (NLRC) in Japanese flounder (Paralichthys olivaceus)
    • Unajak S., Santos M.D., Hikima J., Jang T., Kondo H., Hirono I., et al. Molecular characterization, expression and functional analysis of a nuclear oligomerization domain proteins subfamily C (NLRC) in Japanese flounder (Paralichthys olivaceus). Fish Shellfish Immunol 2011, 31:201-211.
    • (2011) Fish Shellfish Immunol , vol.31 , pp. 201-211
    • Unajak, S.1    Santos, M.D.2    Hikima, J.3    Jang, T.4    Kondo, H.5    Hirono, I.6
  • 35
    • 0000327905 scopus 로고
    • Isolation and preliminary identification of the causative agent of carp erythrodermatitis
    • Bootsma R., Fijan N., Blommaert J. Isolation and preliminary identification of the causative agent of carp erythrodermatitis. Vet Arh 1977, 47:291-302.
    • (1977) Vet Arh , vol.47 , pp. 291-302
    • Bootsma, R.1    Fijan, N.2    Blommaert, J.3
  • 37
    • 0001013866 scopus 로고
    • Aeromonas hydrophila septicemia of Indian major carps in some commercial fish farms of West Godavari District, Andhra Pradesh
    • Karunasagar I., Rosalind G., Gopal M., Rao K. Aeromonas hydrophila septicemia of Indian major carps in some commercial fish farms of West Godavari District, Andhra Pradesh. Curr Sci 1989, 58:1044-1045.
    • (1989) Curr Sci , vol.58 , pp. 1044-1045
    • Karunasagar, I.1    Rosalind, G.2    Gopal, M.3    Rao, K.4
  • 38
    • 0012513974 scopus 로고    scopus 로고
    • Etiological charac-terization of acute infectious abdominal dropsy outbreak affecting Indian major carp, Cirrhinus mrigala in South Andaman
    • Shome R.B., Shome R., Sarangi N., Bandyopadhyay N.K. Etiological charac-terization of acute infectious abdominal dropsy outbreak affecting Indian major carp, Cirrhinus mrigala in South Andaman. Curr Sci 1996, 70(8):744-747.
    • (1996) Curr Sci , vol.70 , Issue.8 , pp. 744-747
    • Shome, R.B.1    Shome, R.2    Sarangi, N.3    Bandyopadhyay, N.K.4
  • 40
    • 0027968068 scopus 로고
    • Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
    • Thompson J.D., Higgins D.G., Gibson T.J., Clustal W. improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22:4673-4680.
    • (1994) Nucleic Acids Res , vol.22 , pp. 4673-4680
    • Thompson, J.D.1    Higgins, D.G.2    Gibson, T.J.3    Clustal, W.4
  • 41
    • 34547781750 scopus 로고    scopus 로고
    • MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0
    • Tamura k, Dudley J., Nei M., Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.
    • (2007) Mol Biol Evol , vol.24 , pp. 1596-1599
    • Tamura, K.1    Dudley, J.2    Nei, M.3    Kumar, S.4
  • 42
    • 34347209992 scopus 로고    scopus 로고
    • Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors
    • Matsushima N., Tanaka T., Enkhbayar P., Mikami T., Taga M., Yamada K., et al. Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors. BMC Genomics 2007, 8:124.
    • (2007) BMC Genomics , vol.8 , pp. 124
    • Matsushima, N.1    Tanaka, T.2    Enkhbayar, P.3    Mikami, T.4    Taga, M.5    Yamada, K.6
  • 44
    • 0347823003 scopus 로고    scopus 로고
    • MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences
    • Campanella J.J., Bitincka L., Smalley J. MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinform 2003, 4:29.
    • (2003) BMC Bioinform , vol.4 , pp. 29
    • Campanella, J.J.1    Bitincka, L.2    Smalley, J.3
  • 45
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method
    • Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method. Methods 2001, 25:402-408.
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 46
    • 77249101479 scopus 로고    scopus 로고
    • Host-microb interactions in the developing zebrafish
    • Kanther M., Rawls J.F. Host-microb interactions in the developing zebrafish. Curr Opin Immunol 2010, 22:10-19.
    • (2010) Curr Opin Immunol , vol.22 , pp. 10-19
    • Kanther, M.1    Rawls, J.F.2
  • 48
    • 79953327085 scopus 로고    scopus 로고
    • Ontogeny and tissue-specific expression of innate immune related genes in rohu, Labeo rohita (Hamilton)
    • Nayak S.P., Mohanty B.R., Mishra J., Rauta P.R., Das A., Eknath A.E., et al. Ontogeny and tissue-specific expression of innate immune related genes in rohu, Labeo rohita (Hamilton). Fish Shellfish Immunol 2011, 30:1197-1201.
    • (2011) Fish Shellfish Immunol , vol.30 , pp. 1197-1201
    • Nayak, S.P.1    Mohanty, B.R.2    Mishra, J.3    Rauta, P.R.4    Das, A.5    Eknath, A.E.6
  • 49
    • 80054963115 scopus 로고    scopus 로고
    • Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu (Labeo rohita)
    • Swain B., Basu M., Sahoo B.R., Maiti N.K., Routray P., Eknath A.E., et al. Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu (Labeo rohita). Dev Comp Immunol 2012, 36:93-103.
    • (2012) Dev Comp Immunol , vol.36 , pp. 93-103
    • Swain, B.1    Basu, M.2    Sahoo, B.R.3    Maiti, N.K.4    Routray, P.5    Eknath, A.E.6
  • 50
    • 70349978304 scopus 로고    scopus 로고
    • Structure and expression pattern of teleost caspase recruitment domain (CARD) containing protein that are potentially involved in NF-κB signaling
    • Chang M.X., Chen W.Q., Nie P. Structure and expression pattern of teleost caspase recruitment domain (CARD) containing protein that are potentially involved in NF-κB signaling. Dev Comp Immunol 2010, 34:1-13.
    • (2010) Dev Comp Immunol , vol.34 , pp. 1-13
    • Chang, M.X.1    Chen, W.Q.2    Nie, P.3
  • 51
    • 33744969808 scopus 로고    scopus 로고
    • Up-regulation of NOD1 and NOD2 through TLR4 and TNF-α in LPS-treated murine macrophages
    • Takahashi Y., Isuzugawa K., Murase Y., Imai M., Yamamoto S., Iizuka M., et al. Up-regulation of NOD1 and NOD2 through TLR4 and TNF-α in LPS-treated murine macrophages. J Vet Med Sci 2006, 68:471-478.
    • (2006) J Vet Med Sci , vol.68 , pp. 471-478
    • Takahashi, Y.1    Isuzugawa, K.2    Murase, Y.3    Imai, M.4    Yamamoto, S.5    Iizuka, M.6
  • 52
    • 34247177912 scopus 로고    scopus 로고
    • Role of nods in bacterial infection
    • Bourhis L.L., Werts C. Role of nods in bacterial infection. Microbes Infect 2007, 9:629-636.
    • (2007) Microbes Infect , vol.9 , pp. 629-636
    • Bourhis, L.L.1    Werts, C.2
  • 53
    • 29244441151 scopus 로고    scopus 로고
    • Induction and localization of NOD2 protein in human endothelial cells
    • Oh H.M., Lee H.J., Seo G.S., Choi E.Y., Kweon S.H., Chun C.H., et al. Induction and localization of NOD2 protein in human endothelial cells. Cell Immunol 2005, 237:37-44.
    • (2005) Cell Immunol , vol.237 , pp. 37-44
    • Oh, H.M.1    Lee, H.J.2    Seo, G.S.3    Choi, E.Y.4    Kweon, S.H.5    Chun, C.H.6
  • 54
    • 43749095152 scopus 로고    scopus 로고
    • Regulation and functional impact of lipopolysaccharide induced Nod2 gene expression in the murine epididymal epithelial cell line PC1
    • Muhlbauer M., Cheely A.W., Yenugu S., Jobin C. Regulation and functional impact of lipopolysaccharide induced Nod2 gene expression in the murine epididymal epithelial cell line PC1. Immunology 2008, 124:256-264.
    • (2008) Immunology , vol.124 , pp. 256-264
    • Muhlbauer, M.1    Cheely, A.W.2    Yenugu, S.3    Jobin, C.4
  • 55
    • 0042858341 scopus 로고    scopus 로고
    • Interferon-gamma augments CARD4/NOD1 gene and protein expression through interferon regulatory factor-1 in intestinal epithelial cells
    • Hisamatsu T., Suzuki M., Podolsky D.K. Interferon-gamma augments CARD4/NOD1 gene and protein expression through interferon regulatory factor-1 in intestinal epithelial cells. J Biol Chem 2003, 278:32962-32968.
    • (2003) J Biol Chem , vol.278 , pp. 32962-32968
    • Hisamatsu, T.1    Suzuki, M.2    Podolsky, D.K.3


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