Esterases with an Introduced Amidase-Like Hydrogen Bond in the Transition State Have Increased Amidase Specificity

ChemBioChem

Volumn 13, Issue 5, 2012, Pages 645-648

  Syrén, Per Olof ^a,b   Hendil Forssell, Peter ^a   Aumailley, Lucie ^c   Besenmatter, Werner ^d   Gounine, Farida ^c   Svendsen, Allan ^d   Martinelle, Mats ^a   Hult, Karl ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

^b UNIVERSITY OF STUTTGART   (Germany)

^c UNIVERSITÉ DE LA ROCHELLE   (France)

^d NOVOZYMES A S   (Denmark)

Author keywords

Biocatalysis; Chemoselectivity; Enzyme catalysis; Hydrogen bonds; Protein engineering

Indexed keywords

AMIDASE; AMIDE; CUTINASE; ESTERASE; HYDROGEN; LIPASE B;

ARTICLE; BACTERIUM; CANDIDA ANTARCTICA; GENETIC VARIABILITY; HUMICOLA INSOLENS; HYDROGEN BOND; HYDROLYSIS; HYPOTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

AMIDES; AMIDOHYDROLASES; COMPUTATIONAL BIOLOGY; ESTERASES; ESTERS; HYDROGEN BONDING; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; SUBSTRATE SPECIFICITY;

EID: 84858306550     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201100779     Document Type: Article

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