A BRET assay for monitoring insulin receptor interactions and ligand pharmacology

Journal of Receptors and Signal Transduction

Volumn 32, Issue 2, 2012, Pages 57-64

  Kulahin, Nikolaj ^a   Sanni, Samra J ^b,c   Slaaby, Rita ^b   Nøhr, Jane ^a   Gammeltoft, Steen ^c   Hansen, Jakob Lerche ^b,d   Jorgensen, Rasmus ^b  

^a HAGEDORN RESEARCH INSTITUTE   (Denmark)

^b NOVO NORDISK A S   (Denmark)

^c GLOSTRUP UNIVERSITY HOSPITAL   (Denmark)

^d UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

BRET; Insulin receptor signaling; IRS1; IRS4; Shc

Indexed keywords

ASPARTIC ACID; GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN 2; HISTIDINE; INSULIN DERIVATIVE; INSULIN RECEPTOR; INSULIN RECEPTOR SUBSTRATE; INSULIN RECEPTOR SUBSTRATE 1; INSULIN RECEPTOR SUBSTRATE 4; INSULIN X10; PROTEIN SHC; RENILLA LUCIFERIN 2 MONOOXYGENASE; UNCLASSIFIED DRUG;

BINDING AFFINITY; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL STRAIN HEK293; ENZYME SUBSTRATE; GENETIC TRANSFECTION; LIGAND BINDING; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REVIEW; SIGNAL TRANSDUCTION;

CELLS, CULTURED; GREEN FLUORESCENT PROTEINS; HUMANS; INSULIN; INSULIN RECEPTOR SUBSTRATE PROTEINS; KIDNEY; LUMINESCENT MEASUREMENTS; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECEPTOR, INSULIN; RECOMBINANT FUSION PROTEINS; SHC SIGNALING ADAPTOR PROTEINS;

RENILLA RENIFORMIS;

EID: 84858254461     PISSN: 10799893     EISSN: 15324281     Source Type: Journal    
DOI: 10.3109/10799893.2011.647351     Document Type: Review

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