Glutamate 268 regulates transport probability of the anion/proton exchanger ClC-5

Journal of Biological Chemistry

Volumn 287, Issue 11, 2012, Pages 8101-8109

  Grieschat, Matthias ^a   Alekov, Alexi K ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANION-BINDING; CHARGE MOVEMENT; CONSERVED RESIDUES; CURRENT AMPLITUDE; EXTRACELLULAR; INTERNAL PH; NONLINEAR CAPACITANCE; SULFHYDRYL; TRANSPORT CURRENTS; TRANSPORT CYCLE; VOLTAGE DEPENDENCE;

MAMMALS; PROBABILITY; PROTONATION;

PROTONS;

CHLORIDE CHANNEL; CHLORIDE ION; CIC 5 PROTEIN; GLUTAMIC ACID; MUTANT PROTEIN; PROTON; THIOCYANATE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ACIDITY; AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; HUMAN; HUMAN CELL; PH; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN TRANSPORT; PROTON TRANSPORT; REGULATORY MECHANISM;

AMINO ACID SUBSTITUTION; BINDING SITES; CHLORIDE CHANNELS; GLUTAMIC ACID; HEK293 CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; ION TRANSPORT; MUTATION, MISSENSE; PROTONS; SULFHYDRYL COMPOUNDS;

MAMMALIA;

EID: 84858018450     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.298265     Document Type: Article

References (36)

1. Scheel, O., Zdebik, A. A., Lourdel, S., and Jentsch, T. J. (2005) Voltage-dependent electrogenic chloride/proton exchange by endosomal CLC proteins. Nature 436, 424-427 (Pubitemid 41059055)
2. Picollo, A., and Pusch, M. (2005) Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5. Nature 436, 420-423 (Pubitemid 41059054)
3. De Angeli, A., Monachello, D., Ephritikhine, G., Frachisse, J. M., Thomine, S., Gambale, F., and Barbier-Brygoo, H. (2006) The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles. Nature 442, 939-942 (Pubitemid 44285951)
4. + antiporter ClC-7 is the primary chloride permeation pathway in lysosomes. Nature 453, 788-792
5. + antiporter becomes uncoupled at low extracellular pH. J. Biol. Chem. 285, 2569-2579
6. Neagoe, I., Stauber, T., Fidzinski, P., Bergsdorf, E. Y., and Jentsch, T. J. (2010) The late endosomal ClC-6 mediates proton/chloride countertransport in heterologous plasma membrane expression. J. Biol. Chem. 285, 21689-21697
7. Lloyd, S. E., Pearce, S. H., Fisher, S. E., Steinmeyer, K., Schwappach, B., Scheinman, S. J., Harding, B., Bolino, A., Devoto, M., Goodyer, P., Rigden, S. P., Wrong, O., Jentsch, T. J., Craig, I. W., and Thakker, R. V. (1996) A common molecular basis for three inherited kidney stone diseases. Nature 379, 445-449 (Pubitemid 26039523)
8. --channel disruption impairs endocytosis in a mouse model for Dent disease. Nature 408, 369-373
9. Hara-Chikuma, M., Wang, Y., Guggino, S. E., Guggino, W. B., and Verkman, A. S. (2005) Impaired acidification in early endosomes of ClC-5- deficient proximal tubule. Biochem. Biophys. Res. Commun. 329, 941-946 (Pubitemid 40341368)
10. Smith, A. J., Reed, A. A., Loh, N. Y., Thakker, R. V., and Lippiat, J. D. (2009) Characterization of Dent disease mutations of ClC-5 reveals a correlation between functional and cell biological consequences and protein structure. Am. J. Physiol. Renal Physiol. 296, F390-F397
11. Novarino, G., Weinert, S., Rickheit, G., and Jentsch, T. J. (2010) Endosomal chloride/proton exchange rather than chloride conductance is crucial for renal endocytosis. Science 328, 1398-1401
12. + exchange transporter by polyatomic anions. J. Mol. Biol. 362, 682-690
13. Zdebik, A. A., Zifarelli, G., Bergsdorf, E. Y., Soliani, P., Scheel, O., Jentsch, T. J., and Pusch, M. (2008) Determinants of anion/proton coupling in mammalian endosomal CLC proteins. J. Biol. Chem. 283, 4219-4227
14. Alekov, A. K., and Fahlke, Ch. (2009) Channel-like slippage modes in the human anion/proton exchanger ClC-4. J. Gen. Physiol. 133, 485-496
15. + exchanger. J. Gen. Physiol. 126, 563-570
16. + exchanger. J. Gen. Physiol. 133, 131-138
17. + exchanger. FASEB J. 24, 3696-3705
18. Orhan, G., Fahlke, Ch., and Alekov, A. K. (2011) Anion- and proton-dependent gating of CIC-4 anion/proton transporter under uncoupling conditions. Biophys. J. 100, 1233-1241
19. + exchanger and requires Ostm1 for transport activity. EMBO J. 30, 2140-2152
20. + exchanger. J. Gen. Physiol. 135, 653-659
21. Accardi, A., and Miller, C. (2004) Secondary active transport mediated by a prokaryotic homolog of ClC Cl- channels. Nature 427, 803-807 (Pubitemid 38297735)
22. Dutzler, R., Campbell, E. B., and MacKinnon, R. (2003) Gating the selectivity filter in ClC chloride channels. Science 300, 108-112 (Pubitemid 36423038)
23. Feng, L., Campbell, E. B., Hsiung, Y., and MacKinnon, R. (2010) Structure of a eukaryotic CLC transporter defines an intermediate state in the transport cycle. Science 330, 635-641
24. Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., and Tsien, R. Y. (2004) Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567-1572 (Pubitemid 40039460)
25. Graham, F. L., and van der Eb, A. J. (1973)Anew technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456-467
26. Hebeisen, S., Heidtmann, H., Cosmelli, D., Gonzalez, C., Poser, B., Latorre, R., Alvarez, O., and Fahlke, Ch. (2003) Anion permeation in human ClC-4 channels. Biophys. J. 84, 2306-2318 (Pubitemid 36373553)
27. Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391, 85-100 (Pubitemid 11012976)
28. Barry, P. H. (1994) JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial, and bilayer measurements and for correcting junction potential measurements. J. Neurosci. Methods 51, 107-116
29. Gillis, K. D. (2000) Admittance-based measurement of membrane capacitance using the EPC-9 patch-clamp amplifier. Pflugers Arch. 439, 655-664
30. Santos-Sacchi, J. (1991) Reversible inhibition of voltage-dependent outer hair cell motility and capacitance. J. Neurosci. 11, 3096-3110
31. Heinemann, S. H., and Conti, F. (1992) Non-stationary noise analysis and application to patch-clamp recordings. Methods Enzymol. 207, 131-148
32. + antiporter by a single point mutation. EMBO J. 28, 175-182
33. Picollo, A., Malvezzi, M., Houtman, J. C., and Accardi, A. (2009) Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters. Nat. Struct. Mol. Biol. 16, 1294-1301
34. Bossi, E., Centinaio, E., Castagna, M., Giovannardi, S., Vincenti, S., Sacchi, V. F., and Peres, A. (1999) Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes. J. Physiol. 515, 729-742 (Pubitemid 29140108)
35. +/glucose cotransporter (SGLT1). J. Membr. Biol. 155, 175-186
36. Mager, S., Naeve, J., Quick, M., Labarca, C., Davidson, N., and Lester, H. A. (1993) Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron 10, 177-188 (Pubitemid 23079540)