Effects of 2-methoxyestradiol on proliferation, apoptosis and gene expression of cyclin B1 and c-Myc in esophageal carcinoma EC9706 cells

Cell Biochemistry and Function

Volumn 30, Issue 2, 2012, Pages 158-165

  Du, Bin ^a   Zhao, Zhilan ^a   Sun, Huibin ^a   Ma, Sijia ^a   Jin, Jianwen ^a   Zhang, Zhenzhong ^a  

^a ZHENGZHOU UNIVERSITY   (China)

Author keywords

2 methoxyestradiol; Apoptosis; C Myc; Cell cycle; Cyclin B1; Esophageal carcinoma

Indexed keywords

2 METHOXYESTRADIOL; CYCLIN B1; CYCLIN DEPENDENT KINASE 1; MYC PROTEIN;

ANTINEOPLASTIC ACTIVITY; ANTIPROLIFERATIVE ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL COUNT; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE PROGRESSION; CELL PROLIFERATION; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; ESOPHAGUS CARCINOMA; FLOW CYTOMETRY; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; ONCOGENE; PRIORITY JOURNAL; UPREGULATION;

APOPTOSIS; CARCINOMA; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; CYCLIN B1; ESOPHAGEAL NEOPLASMS; ESTRADIOL; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; PROTO-ONCOGENE PROTEINS C-MYC;

EID: 84858005024     PISSN: 02636484     EISSN: 10990844     Source Type: Journal    
DOI: 10.1002/cbf.1830     Document Type: Article

References (27)

1. Parkin DM, Bray F, Ferlay J, et al. Global cancer statistics. CA Cancer J Clin 2002; 55: 74-108.
2. Kollarova H, Machova L, Horakova D. Epidemiology of esophageal cancer-an overview article. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2007; 151: 17-20.
3. Edwards BK, Brown ML, Wingo PA, et al. Annual report to the nation on the status of cancer, 1975-2002, featuring population-based trends in cancer treatment. J Natl Cancer Inst 2005; 97: 1407-1427.
4. Sutherland TE, Anderson RL, Hughes RA, et al. 2-Methoxyestradiol-a unique blend of activities generating a new class of anti-tumour/anti-inflammatory agents. Drug Discov Today 2007; 12: 577-584.
5. Pribluda VS, Gubish ER, Lavallee TM, et al. 2-Methoxyestradiol: an endogenous antiangiogenic and antiproliferative drug candidate. Cancer Metastasis Rev 2000; 19: 173-179.
6. Mooberry SL. Mechanism of action of 2-methoxyestradiol: newdevelopments. Drug Resist Update 2003; 6: 355-361.
7. Bhati R, Gökmen-Polar Y, Sledge GW, et al. 2-Methoxyestradiol inhibits the anaphase-promoting complex and protein translation in human breast cancer cells. Cancer Res 2007; 67: 702-708.
8. Liaw TY, Salam NK, McKa MJ, et al. Class I beta-tubulin mutations in 2-methoxyestradiol-resistant acute lymphoblastic leukemia cells: implications for drug-target interactions. Mol Cancer Ther 2008; 7: 3150-3159.
9. Thaver V, Lottering ML, Papendorp D, et al. In vitro effects of 2-methoxyestradiol on cell numbers, morphology, cell cycle progression, and apoptosis induction in oesophageal carcinoma cells. Cell Biochem Funct 2009; 27: 205-210.
10. Joubert A, Maritz C, Joubert F. Bax/Bcl-2 expression levels of 2-methoxyestradiol-exposed esophageal cancer cells. Biomed Res 2005; 26: 131-134.
11. Joubert A, Marais S. Influence of 2-methoxyestradiol on cell morphology and cdc2 kinase activity in WHCO3 esophageal carcinoma cells. Biomed Res 2007; 28: 9-16.
12. Mosmanna T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
13. Zhou L, Hou J, Fu W, et al. Arsenic trioxide and 2-methoxyestradiol reduce β-catenin accumulation after proteasome inhibition and enhance the sensitivity of myeloma cells to Bortezomib. Leuk Res 2008; 32: 1674-1683.
14. 2/M cell-cycle arrest of nasopharyngeal carcinoma cells. Cancer Lett 2008; 268: 295-307.
15. Zhang CE, Victoria R, Ni X, Rakhshandra T, Madeleine D. Selective induction of apoptosis by histone deacetylase inhibitor SAHA in cutaneous T-cell lymphoma cells: relevance to mechanism of therapeutic action. J Invest Dermatol 2005; 125: 1045-1052.
16. Zor T, Selinger Z. Linearization of the bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal Biochem 1996; 236: 302-308.
17. Lee SC, Chan JY, Pervaiz S. Spontaneous and 5-fluorouracil-induced centrosome amplification lowers the threshold to resveratrol-evoked apoptosis in colon cancer cells. Cancer Lett 2010; 288: 36-41.
18. Hou GQ, Xue LX, Lu ZM, et al. An activated mTORp70S6K signaling pathway in esophageal squamous cell carcinoma cell lines and inhibitionof the pathway by rapamycin and siRNA against mTOR. Cancer Lett 2007; 253: 236-248.
19. Chow JM, Liu CR, Lin CP, et al. Downregulation of c-myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia. Exp Hematol 2008; 36: 140-148.
20. Zhou X, Jiang H, Hou J. Coordination of upregulated XBP-1 and downregulated c-myc during myeloma cell differentiation induced by 2-methoxyestradiol. Leukemia Res 2007; 31: 1259-1265.
21. Gomez LA, Las Pozas A, Reiner T, et al. Increased expression of cyclin B1 sensitizes prostate cancer cells to apoptosis induced by chemotherapy. Mol Cancer Ther 2007; 6: 1534-1543.
22. Reiner T, Pozas A, Gomez LA, et al. Low dose combinations of 2-methoxyestradiol and docetaxel block prostate cancer cells in mitosis and increase apoptosis. Cancer Lett 2009; 276: 21-31.
23. Stander BA, Marais S, Vorster CJ, et al. In vitro effects of 2-methoxyestradiol on morphology, cell cycle progression, cell death and gene expression changes in the tumourigenic MCF-7 breast epithelial cell line. J Steroid Biochem Mol Biol 2010; 119: 149-160.
24. Attalla H, Mäkelä TP, Adlercreutz H, Andersson LC. 2-Methoxyestradiol arrests cells in mitosis without depolymerizing tubulin. Biochem Biophys Res Commun 1996; 228: 467-473.
25. 2/M transition. Cell Cycle 2004; 3: 733-737.
26. Baker DL, Dawlaty MM, Galardy P, et al. Mitotic regulation of the anaphase-promoting complex. Cell Mol Life Sci 2007; 64: 589-600.
27. Ibrahim SM, Ringel J, Schmidt C, et al. Pancreatic adenocarcinoma cell lines show variable susceptibility to TRAIL-mediated cell death. Pancreas 2001; 23: 72-79.