Improving the diastereoselectivity of penicillin G acylase for ampicillin synthesis from racemic substrates

Protein Engineering, Design and Selection

Volumn 25, Issue 3, 2012, Pages 135-144

  Deaguero, Andria L ^a,b   Blum, Janna K ^a,b   Bommarius, Andreas S ^a,b  

^a Georgia Institute of Technology   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

diastereoselectivity; penicillin G acylase lactam antibiotics

Indexed keywords

6-AMINOPENICILLANIC ACID; ALPHA CARBON; AMINO GROUP; CHIRAL CENTERS; DIASTEREO-SELECTIVITY; DIASTEREOMERIC EXCESS; FASCINATING PHENOMENA; HYDROLYSIS RATIO; LIBRARY SCREENING; METHYL ESTERS; PENICILLIN G ACYLASE; RACEMASE; RACEMIC SUBSTRATES; SITE DIRECTED MUTAGENESIS; WILD-TYPE ENZYMES;

ANTIBIOTICS; DIAGNOSIS; HYDROLYSIS; STEREOSELECTIVITY;

SUBSTRATES;

6 AMINOPENICILLANIC ACID; AMPICILLIN; EPIMERASE; METHYL GROUP; PENICILLIN AMIDASE; PHENYLGLYCINE; RACEMASE;

AMINO ACID SEQUENCE; ARTICLE; CHIRALITY; DIASTEREOISOMER; DRUG SELECTIVITY; DRUG STRUCTURE; DRUG SYNTHESIS; ENANTIOMER; ENZYME ACTIVITY; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; RACEMIC MIXTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; AMINOBENZOIC ACIDS; AMPICILLIN; CATALYTIC DOMAIN; CHROMOGENIC COMPOUNDS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; NITROBENZOATES; PENICILLIN AMIDASE; RACEMASES AND EPIMERASES; STEREOISOMERISM; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 84857880335     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr065     Document Type: Article

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