A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag

Bioscience, Biotechnology and Biochemistry

Volumn 76, Issue 2, 2012, Pages 388-390

  Nakagawa, Chika ^a   Nishimura, Shigenori ^a   Senda Murata, Kaori ^a   Sugimoto, Kenji ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Fluorescent protein (FP); Importin alpha (importin ); Nuclear pore complex (NPC); Venus; Weak dimer

Indexed keywords

DIMER INTERFACE; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT PROTEIN; FUSION TAG; HYDROPHOBIC INTERACTIONS; LIVING CELL; MAMMALIAN CELLS; MONOMERIZATION; NUCLEAR-PORE COMPLEX (NPC); PHYSIOLOGICAL CONDITION; SIMPLE METHOD; SINGLE MUTATION; VENUS;

DIMERS; FLUORESCENCE;

PROTEINS;

DIAGNOSTIC AGENT; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; KARYOPHERIN ALPHA; PHOTOPROTEIN;

ANIMAL; ARTICLE; CELLS; CHEMICAL PHENOMENA; CHEMISTRY; CYTOLOGY; GENETICS; METHODOLOGY; MISSENSE MUTATION; PROCEDURES; PROTEIN MULTIMERIZATION;

ALPHA KARYOPHERINS; ANIMALS; CELLS; CYTOLOGICAL TECHNIQUES; GREEN FLUORESCENT PROTEINS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LUMINESCENT PROTEINS; METHODS; MUTATION, MISSENSE; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS;

MAMMALIA;

EID: 84857859016     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.110677     Document Type: Article

References (18)

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, and Prasher DC, Science, 263, 802-805 (1994).
2. Sugimoto K, Fukuda R, and Himeno M, Cell Struct. Funct., 25, 253-261 (2000).
3. Sugimoto K, Urano T, Zushi H, Inoue K, Tasaka H, Tachibana M, and Dotsu M, Cell Struct. Funct., 27, 457-467 (2002).
4. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, and Lukyanov SA, Nat. Biotechnol., 17, 969-973 (1999).
5. Champbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, and Tsien RY, Proc. Natl. Acad. Sci. USA, 99, 7877-7882 (2002).
6. Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, and Tsien RY, Nat. Biotechnol., 22, 1567-1572 (2004).
7. Heim R, Cubitt AB, and Tsien RY, Nature, 373, 663-664 (1995).
8. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, and Miyawaki A, Nat. Biotechnol., 20, 87-90 (2002).
9. Shaner NC, Steinbach PA, and Tsien RY, Nat. Methods, 2, 905-909 (2005).
10. Miyamoto Y, Hieda M, Harreman MT, Fukumoto M, Saiwaki T, Hodel AE, Corbett AH, and Yoneda Y, EMBO J., 21, 5833-5842 (2002).
11. Hubner S, Smith HMS, Hu W, Chan CK, Rihs HP, Paschal BM, Raikhel NV, and Jans DA, J. Biol. Chem., 274, 22610-22617 (1999).
12. Fukada T, Inoue K, Urano T, and Sugimoto K, BioTechniques, 37, 552-556 (2004).
13. Karasawa S, Araki T, Nagai T, Mizuno H, and Miyawaki A, Biochem. J., 381, 307-312 (2004).
14. Rehm M, Dussmann H, and Prehn JHM, J. Cell Biol., 162, 1031-1043 (2003).
15. Zacharias DA, Violin JD, Newton AC, and Tsien RY, Science, 296, 913-916 (2002).
16. Sarkar G and Sommer SS, BioTechniques, 8, 404-407 (1990).
17. Nakagawa C, Inahata K, Nishimura S, and Sugimoto K, Biosci. Biotechnol. Biochem., 75, 1399-1401 (2011).
18. Zhang J, Campbell RE, Ting AY, and Tsien RY, Nat. Rev. Mol. Cell Biol., 3, 906-918 (2002).