Improvement of a Venus-Based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in living cells

Bioscience, Biotechnology and Biochemistry

Volumn 75, Issue 7, 2011, Pages 1399-1401

  Nakagawa, Chika ^a   Inahata, Kazuto ^a   Nishimura, Shigenori ^a   Sugimoto, Kenji ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Bimolecular fluorescence complementation (BiFC); Fluorescent protein; Protein interactions; Signal to noise (S N) ratio; Venus

Indexed keywords

COMPLEMENTATION; FLUORESCENT PROTEIN; PROTEIN INTERACTIONS; SIGNAL TO NOISE; VENUS;

PROTEINS; SIGNAL TO NOISE RATIO;

FLUORESCENCE;

BACTERIAL PROTEIN; FLUORESCENT DYE; PHOTOPROTEIN; PROTEIN C FOS; PROTEIN C JUN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; BIOASSAY; CHEMISTRY; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; METHODOLOGY; POINT MUTATION; PROTEIN ANALYSIS; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

BACTERIAL PROTEINS; BIOLOGICAL ASSAY; FLUORESCENT DYES; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTO-ONCOGENE PROTEINS C-FOS; PROTO-ONCOGENE PROTEINS C-JUN; TRANSFECTION;

EID: 79960792495     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.110189     Document Type: Article

References (15)

1. Kerppola TK, Annu. Rev. Biophys., 37, 465-487 (2008).
2. Ghosh I, Hamilton AD, and Regan L, J. Am. Chem. Soc., 122, 5658-5659 (2000).
3. Hu CD, Chinenov Y, and Kerppola TK, Mol. Cell, 9, 789-798 (2002).
4. Hu CD and Kerppola TK, Nat. Biotechnol., 21, 539-545 (2003).
5. Shyu YJ, Liu H, Deng X, and Hu CD, BioTechniques, 40, 61-66 (2006).
6. Jach G, Pesch M, Richter K, Frings S, and Uhrig JF, Nat. Methods, 3, 597-600 (2006).
7. Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, and Zhang XE, Biochem. Biophys. Res. Commun., 367, 47-53 (2008).
8. Chu J, Zhang Z, Zheng Y, Yang J, Qin L, Lu J, Huang ZL, Zeng S, and Luo Q, Biosens. Bioelectron., 25, 234-239 (2009).
9. Lee YR, Park JH, Hahm SH, Kang LW, Chung JH, Nam KH, Hwang KY, Kwon IC, and Han YS, Mol. Imaging Biol., 12, 468-478 (2010).
10. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, and Miyawaki A, Nat. Biotechnol., 20, 87-90 (2002).
11. Robida AM and Kerppola TK, J. Mol. Biol., 394, 391-409 (2009).
12. Lin J, Wang N, Li Y, Liu Z, Tian S, Zhao L, Zheng Y, Liu S, Li S, Jin C, and Xia B, Biotech. Histochem., May 14 [Epub ahead of print] (2010).
13. Kodama Y and Hu CD, BioTechniques, 49, 793-805 (2010).
14. Sugimoto K, Urano T, Zushi H, Inoue K, Tasaka H, Tachibana M, and Dotsu M, Cell Struct. Funct., 27, 457-467 (2002).
15. Yasui M, Koyama N, Koizumi T, Senda-Murata K, Takashima Y, Hayashi M, Sugimoto K, and Honma M, Mutat. Res., 692, 12-18 (2010).