Low-frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Bioelectromagnetics

Volumn 33, Issue 3, 2012, Pages 226-237

  Bouwens, Mark ^a   de Kleijn, Stan ^b   Ferwerda, Gerben ^b   Cuppen, Jan J ^c   Savelkoul, Huub F J ^a   Kemenade, B M Lidy Verburg van ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^c Immunent BV   (Netherlands)

Author keywords

Cytokines; LF EMF; Macrophages; Monocytes; THP 1

Indexed keywords

BODY FLUIDS; CELL CULTURE; ELECTROMAGNETIC FIELD EFFECTS; ELECTROMAGNETIC FIELDS; GENE EXPRESSION; PROTEINS;

CELL LINES; CYTOKINES; GENES EXPRESSION; HUMAN HEALTH; HUMAN MONOCYTES; IMMUNE CELLS; INFLAMMATORY GENES; LOW FREQUENCY ELECTROMAGNETIC FIELD; MONOCYTE; THP-1;

MACROPHAGES;

CYTOKINE; INTERLEUKIN 1BETA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BIOSYNTHESIS; ELECTROMAGNETIC FIELD; EPITHELIUM CELL; GENE EXPRESSION; HUMAN; INFLAMMATION; INNATE IMMUNITY; MACROPHAGE; MONOCYTE; RADIATION EXPOSURE; TUMOR CELL LINE;

CELL LINE, TUMOR; CYTOKINES; ELECTROMAGNETIC FIELDS; EPITHELIAL CELLS; GENE EXPRESSION; HUMANS; IMMUNITY, INNATE; INFLAMMATION; INTERLEUKIN-1BETA; MACROPHAGES; MONOCYTES; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84857797504     PISSN: 01978462     EISSN: 1521186X     Source Type: Journal    
DOI: 10.1002/bem.20695     Document Type: Article

References (39)

1. Ahlbom A, Bridges J, de Seze R, Hillert L, Juutilainen J, Mattsson MO, Neubauer G, Schuz J, Simko M, Bromen K. 2008. Possible effects of electromagnetic fields (EMF) on human health-opinion of the Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR). Toxicology 246(2-3): 248-250.
2. Akan Z, Aksu B, Tulunay A, Bilsel S, Inhan-Garip A. 2010. Extremely low-frequency electromagnetic fields affect the immune response of monocyte-derived macrophages to pathogens. Bioelectromagnetics 31: 603-612.
3. Barksby HE, Nile CJ, Jaedicke KM, Taylor JJ, Preshaw PM. 2009. Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clin Exp Immunol 156(3): 479-487.
4. Blank M, Soo L. 2001. Optimal frequencies for magnetic acceleration of cytochrome oxidase and Na, K-ATPase reactions. Bioelectrochemistry 53(2): 171-174.
5. Boscolo P, Bergamaschi A, Di Sciascio MB, Benvenuti F, Reale M, Di Stefano F, Conti P, Di Gioacchino M. 2001. Effects of low frequency electromagnetic fields on expression of lymphocyte subsets and production of cytokines of men and women employed in a museum. Sci Total Environ 270(1-3): 13-20.
6. Callaghan MJ, Chang EI, Seiser N, Aarabi S, Ghali S, Kinnucan ER, Simon BJ, Gurtner GC. 2008. Pulsed electromagnetic fields accelerate normal and diabetic wound healing by increasing endogenous FGF-2 release. Plast Reconstr Surg 121(1): 130-141.
7. Chanput W, Mes J, Vreeburg RAM, Savelkoul H, Wichers HJ. 2010. Transcription profiles of LPS-stimulated THP-1 monocytes and macrophages: A tool to study inflammation modulating effects of food-derived compounds. Food Funct 1: 254-261.
8. Cossarizza A, Monti D, Bersani F, Paganelli R, Montagnani G, Cadossi R, Cantini M, Franceschi C. 1989. Extremely low frequency pulsed electromagnetic fields increase interleukin-2 (IL-2) utilization and IL-2 receptor expression in mitogen-stimulated human lymphocytes from old subjects. FEBS Lett 248(1-2): 141-144.
9. Cossarizza A, Angioni S, Petraglia F, Genazzani AR, Monti D, Capri M, Bersani F, Cadossi R, Franceschi C. 1993. Exposure to low frequency pulsed electromagnetic fields increases interleukin-1 and interleukin-6 production by human peripheral blood mononuclear cells. Exp Cell Res 204(2): 385-387.
10. Cuppen J, Wiegertjes G, Lobee H, Savelkoul H, Elmusharaf M, Beynen A, Grooten H, Smink W. 2007. Immune stimulation in fish and chicken through weak low frequency electromagnetic fields. Environmentalist 27: 577-583.
11. D'Acquisto F, May MJ, Ghosh S. 2002. Inhibition of nuclear factor kappa B (NF-B): An emerging theme in anti-inflammatory therapies. Mol Interv 2(1): 22-35.
12. Elmusharaf MA, Cuppen JJ, Grooten HN, Beynen AC. 2007. Antagonistic effect of electromagnetic field exposure on coccidiosis infection in broiler chickens. Poult Sci 86(10): 2139-2143.
13. Frahm J, Mattsson MO, Simko M. 2010. Exposure to ELF magnetic fields modulate redox related protein expression in mouse macrophages. Toxicol Lett 192(3): 330-336.
14. Gaetani R, Ledda M, Barile L, Chimenti I, De Carlo F, Forte E, Ionta V, Giuliani L, D'Emilia E, Frati G, Miraldi F, Pozzi D, Messina E, Grimaldi S, Giacomello A, Lisi A. 2009. Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields. Cardiovasc Res 82(3): 411-420.
15. Goudarzi I, Hajizadeh S, Salmani ME, Abrari K. 2010. Pulsed electromagnetic fields accelerate wound healing in the skin of diabetic rats. Bioelectromagnetics 31(4): 318-323.
16. Grana DR, Marcos HJ, Kokubu GA. 2008. Pulsed electromagnetic fields as adjuvant therapy in bone healing and peri-implant bone formation: An experimental study in rats. Acta Odontol Latinoam 21(1): 77-83.
17. Griffin XL, Warner F, Costa M. 2008. The role of electromagnetic stimulation in the management of established non-union of long bone fractures: What is the evidence? Injury 39(4): 419-429.
18. Ikeda K, Shinmura Y, Mizoe H, Yoshizawa H, Yoshida A, Kanao S, Sumitani H, Hasebe S, Motomura T, Yamakawa T, Mizuno F, Otaka Y, Hirose H. 2003. No effects of extremely low frequency magnetic fields found on cytotoxic activities and cytokine production of human peripheral blood mononuclear cells in vitro. Bioelectromagnetics 24(1): 21-31.
19. Jasti AC, Wetzel BJ, Aviles H, Vesper DN, Nindl G, Johnson MT. 2001. Effect of a wound healing electromagnetic field on inflammatory cytokine gene expression in rats. Biomed Sci Instrum 37: 209-214.
20. Jonai H, Villanueva MB, Yasuda A. 1996. Cytokine profile of human peripheral blood mononuclear cells exposed to 50Hz EMF. Ind Health 34(4): 359-368.
21. Kleijn SD, Bouwens M, Verburg-van Kemenade BM, Cuppen JJ, Ferwerda G, Hermans PW. 2011. Extremely low frequency electromagnetic field exposure does not modulate toll-like receptor signaling in human peripheral blood mononuclear cells. Cytokine 54(1): 43-50.
22. Liburdy RP, Callahan DE, Harland J, Dunham E, Sloma TR, Yaswen P. 1993. Experimental evidence for 60 Hz magnetic fields operating through the signal transduction cascade. Effects on calcium influx and c-MYC mRNA induction. FEBS Lett 334(3): 301-308.
23. Luceri C, De Filippo C, Giovannelli L, Blangiardo M, Cavalieri D, Aglietti F, Pampaloni M, Andreuccetti D, Pieri L, Bambi F, Biggeri A, Dolara P. 2005. Extremely low-frequency electromagnetic fields do not affect DNA damage and gene expression profiles of yeast and human lymphocytes. Radiat Res 164(3): 277-285.
24. Lupke M, Frahm J, Lantow M, Maercker C, Remondini D, Bersani F, Simko M. 2006. Gene expression analysis of ELF-MF exposed human monocytes indicating the involvement of the alternative activation pathway. Biochim Biophys Acta 1763(4): 402-412.
25. Miller SC, Haberer J, Venkatachalam U, Furniss MJ. 1999. NF-kappaB or AP-1-dependent reporter gene expression is not altered in human U937cells exposed to power-line frequency magnetic fields. Radiat Res 151(3): 310-318.
26. Murabayashi S, Yoshikawa A, Mitamura Y. 2004. Functional modulation of activated lymphocytes by time-varying magnetic fields. Ther Apher Dial 8(3): 206-211.
27. Natarajan M, Nayak BK, Galindo C, Mathur SP, Roldan FN, Meltz ML. 2006. Nuclear translocation and DNA-binding activity of NFKB (NF-kappaB) after exposure of human monocytes to pulsed ultra-wideband electromagnetic fields (1kV/cm) fails to transactivate kappaB-dependent gene expression. Radiat Res 165(6): 645-654.
28. Pessina GP, Aldinucci C. 1997. Short cycles of both static and pulsed electromagnetic fields have no effect on the induction of cytokines by peripheral blood mononuclear cells. Bioelectromagnetics 18(8): 548-554.
29. Pessina GP, Aldinucci C. 1998. Pulsed electromagnetic fields enhance the induction of cytokines by peripheral blood mononuclear cells challenged with phytohemagglutinin. Bioelectromagnetics 19(8): 445-451.
30. Pilla AA. 2006. Mechanisms and therapeutic applications of time-varying and static magnetic fields. Barnes F, Greenebaum B (eds.). Boca Raton, FL: CRC Press. 60 pp.
31. Reale M, De Lutiis MA, Patruno A, Speranza L, Felaco M, Grilli A, Macri MA, Comani S, Conti P, Di Luzio S. 2006. Modulation of MCP-1 and iNOS by 50-Hz sinusoidal electromagnetic field. Nitric Oxide 15(1): 50-57.
32. Rollwitz J, Lupke M, Simko M. 2004. Fifty-hertz magnetic fields induce free radical formation in mouse bone marrow-derived promonocytes and macrophages. Biochim Biophys Acta 1674(3): 231-238.
33. Spandidos A, Wang X, Wang H, Seed B. 2010. PrimerBank: A resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 38(Database issue): D792-799.
34. Stokes RW, Doxsee D. 1999. The receptor-mediated uptake, survival, replication, and drug sensitivity of Mycobacterium tuberculosis within the macrophage-like cell line THP-1: A comparison with human monocyte-derived macrophages. Cell Immunol 197(1): 1-9.
35. Tokalov SV, Gutzeit HO. 2004. Weak electromagnetic fields (50 Hz) elicit a stress response in human cells. Environ Res 94(2): 145-151.
36. Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K. 1980. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer 26(2): 171-176.
37. Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K. 1982. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res 42(4): 1530-1536.
38. Vianale G, Reale M, Amerio P, Stefanachi M, Di Luzio S, Muraro R. 2008. Extremely low frequency electromagnetic field enhances human keratinocyte cell growth and decreases proinflammatory chemokine production. Br J Dermatol 158(6): 1189-1196.
39. WHO. 2007. Exposure to extremely low frequency fields. International EMF Project Fact Sheet No. 322. World Health Organization: Geneva, Switzerland. (Last accessed 13 May 2011).