Karyotyping of human chondrocytes in scaffold-assisted cartilage tissue engineering

Acta Biomaterialia

Volumn 8, Issue 4, 2012, Pages 1519-1529

  Trimborn, Marc ^a   Endres, Michaela ^a,b   Bommer, Christiane ^a   Janke, Una ^a   Krüger, Jan Philipp ^b   Morawietz, Lars ^c   Kreuz, Peter C ^d   Kaps, Christian ^a,b  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b TRANSTISSUE TECHNOLOGIES GMBH   (Germany)

^c KATHARINENHOSPITAL   (Germany)

^d ROSTOCK UNIVERSITY MEDICAL CENTER   (Germany)

Author keywords

Cartilage regeneration; Cartilage tissue engineering; Chondrocyte differentiation; Karyotype; Resorbable polymer scaffold

Indexed keywords

CARTILAGE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; REPAIR; SCAFFOLDS (BIOLOGY); TISSUE CULTURE; TISSUE REGENERATION;

AUTOLOGOUS CHONDROCYTE IMPLANTATIONS; CARTILAGE REGENERATION; CARTILAGE TISSUE ENGINEERING; CHONDROCYTE DIFFERENTIATION; CHONDROCYTES; HUMAN CHONDROCYTE; POLY(GLYCOLIC ACID); POLYMER SCAFFOLDS; RESORBABLE; RESORBABLE POLYMER SCAFFOLD;

BODY FLUIDS;

COLLAGEN TYPE 2; FIBRIN; POLYGLYCOLIC ACID;

ADULT; ARTICLE; AUTOLOGOUS CHONDROCYTE IMPLANTATION; C BANDING; CADAVER; CARTILAGE CELL; CELL COUNT; CELL DIFFERENTIATION; CELL EXPANSION; CELL STRUCTURE; CELL TRANSPLANTATION; CHROMOSOME; CHROMOSOME ABERRATION; CONTROLLED STUDY; CRYOPRESERVATION; CYTOGENETICS; ELECTRON MICROSCOPY; EXTRACELLULAR MATRIX; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION; HISTOLOGY; HUMAN; HUMAN CELL; KARYOTYPE; KARYOTYPING; LIVING DONOR; MALE; PRIORITY JOURNAL; TISSUE CULTURE; TISSUE ENGINEERING;

EID: 84857787464     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2011.12.024     Document Type: Article

References (52)

1. M. Brittberg, A. Lindahl, and A. Nilsson Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation N Engl J Med 331 1994 889 895 (Pubitemid 24299869)
2. U. Horas, R. Schnettler, and D. Pelinkovic Osteochondral transplantation versus autogenous chondrocyte transplantation. A prospective comparative clinical study Chirurg 71 2000 1090 1097
3. J.R. Steadman, W.G. Rodkey, and J.J. Rodrigo Microfracture: surgical technique and rehabilitation to treat chondral defects Clin Orthop Relat Res 391 2001 S362 S369
4. G. Bentley, L.C. Biant, and R.W. Carrington A prospective, randomised comparison of autologous chondrocyte implantation versus mosaicplasty for osteochondral defects in the knee J Bone Joint Surg Br 85 2003 223 230 (Pubitemid 36410545)
5. G. Knutsen, J.O. Drogset, and L. Engebretsen A randomized trial comparing autologous chondrocyte implantation with microfracture. Findings at five years J Bone Joint Surg Am 89 2007 2105 2112 (Pubitemid 47606154)
6. J.B. Moseley Jr., A.F. Anderson, and J.E. Browne Long-term durability of autologous chondrocyte implantation: a multicenter, observational study in US patients Am J Sports Med 38 2010 238 246
7. R.A. Magnussen, W.R. Dunn, and J.L. Carey Treatment of focal articular cartilage defects in the knee: a systematic review Clin Orthop Relat Res 466 2008 952 962
8. D.B. Saris, J. Vanlauwe, and J. Victor Treatment of symptomatic cartilage defects of the knee: characterized chondrocyte implantation results in better clinical outcome at 36 months in a randomized trial compared to microfracture Am J Sports Med 37 Suppl 1 2009 10S 19S
9. H.S. Vasiliadis, and J. Wasiak Autologous chondrocyte implantation for full thickness articular cartilage defects of the knee Cochrane Database Syst Rev 2010 CD003323
10. P. Vavken, and D. Samartzis Effectiveness of autologous chondrocyte implantation in cartilage repair of the knee: a systematic review of controlled trials Osteoarthritis Cartilage 18 2010 857 863
11. K. Zaslav, B. Cole, and R. Brewster A prospective study of autologous chondrocyte implantation in patients with failed prior treatment for articular cartilage defect of the knee: results of the Study of the Treatment of Articular Repair (STAR) clinical trial Am J Sports Med 37 2009 42 55
12. B. Grigolo, L. Roseti, and M. Fiorini Transplantation of chondrocytes seeded on a hyaluronan derivative (hyaff-11) into cartilage defects in rabbits Biomaterials 22 2001 2417 2424 (Pubitemid 32666233)
13. E. Kon, A. Di Martino, and G. Filardo Second-generation autologous chondrocyte transplantation: MRI findings and clinical correlations at a minimum 5-year follow-up Eur J Radiol 79 2011 382 388
14. R. Dorotka, U. Windberger, and K. Macfelda Repair of articular cartilage defects treated by microfracture and a three-dimensional collagen matrix Biomaterials 26 2005 3617 3629 (Pubitemid 40038156)
15. P. Niemeyer, P. Lenz, and P.C. Kreuz Chondrocyte-seeded type I/III collagen membrane for autologous chondrocyte transplantation: prospective 2-year results in patients with cartilage defects of the knee joint Arthroscopy 26 2010 1074 1082
16. D. Barnewitz, M. Endres, and I. Kruger Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts Biomaterials 27 2006 2882 2889 (Pubitemid 43165757)
17. C. Ossendorf, C. Kaps, and P.C. Kreuz Treatment of posttraumatic and focal osteoarthritic cartilage defects of the knee with autologous polymer-based three-dimensional chondrocyte grafts: two year clinical results Arthritis Res Ther 9 2007 R41
18. P.C. Kreuz, S. Muller, and U. Freymann Repair of focal cartilage defects with scaffold-assisted autologous chondrocyte grafts: clinical and biomechanical results 48 months after transplantation Am J Sports Med 3 2011 3
19. EMA Guideline on human cell-based medicinal products. Doc. Ref. EMEA/CHMP/410869/2006, www.ema.europa.eu 2008.
20. D.W. Levine, L. Mondano, and M. Halpin FDA regulatory pathways for knee cartilage repair products Sports Med Arthrosc 16 2008 202 207
21. A. Brandl, P. Angele, and C. Roll Influence of the growth factors PDGF-BB, TGF-beta1 and bFGF on the replicative aging of human articular chondrocytes during in vitro expansion J Orthop Res 28 2010 354 360
22. M. Hameed, C. Ulger, and D. Yasar Genome profiling of chondrosarcoma using oligonucleotide array-based comparative genomic hybridization Cancer Genet Cytogenet 192 2009 56 59
23. M.T. Hemann, M.A. Strong, and L.Y. Hao The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability Cell 107 2001 67 77 (Pubitemid 32972038)
24. J.A. Martin, and J.A. Buckwalter Telomere erosion and senescence in human articular cartilage chondrocytes J Gerontol A Biol Sci Med Sci 56 2001 B172 B179
25. P. Chomczynski A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples Biotechniques 15 1993 532 534 (536-7)
26. J. Winer, C.K. Jung, and I. Shackel Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro Anal Biochem 270 1999 41 49 (Pubitemid 29230718)
27. H. Neitzel, L.M. Neumann, and D. Schindler Premature chromosome condensation in humans associated with microcephaly and mental retardation: a novel autosomal recessive condition Am J Hum Genet 70 2002 1015 1022 (Pubitemid 34259318)
28. P.D. Benya, and J.D. Shaffer Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels Cell 30 1982 215 224 (Pubitemid 13237099)
29. E. Kolettas, L. Buluwela, and M.T. Bayliss Expression of cartilage-specific molecules is retained on long-term culture of human articular chondrocytes J Cell Sci 108 1995 1991 1999
30. K. Benz, S. Breit, and M. Lukoschek Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes Biochem Biophys Res Commun 293 2002 284 292 (Pubitemid 34694204)
31. C. Kaps, S. Frauenschuh, and M. Endres Gene expression profiling of human articular cartilage grafts generated by tissue engineering Biomaterials 27 2006 3617 3630
32. M. Sittinger, J. Bujia, and W.W. Minuth Engineering of cartilage tissue using bioresorbable polymer carriers in perfusion culture Biomaterials 15 1994 451 456 (Pubitemid 24151055)
33. F. Zaucke, R. Dinser, and P. Maurer Cartilage oligomeric matrix protein (COMP) and collagen IX are sensitive markers for the differentiation state of articular primary chondrocytes Biochem J 358 2001 17 24 (Pubitemid 32778093)
34. M. Endres, K. Neumann, and S.E. Schroder Human polymer-based cartilage grafts for the regeneration of articular cartilage defects Tissue Cell 39 2007 293 301 (Pubitemid 47500759)
35. P.C. Kreuz, S. Muller, and C. Ossendorf Treatment of focal degenerative cartilage defects with polymer-based autologous chondrocyte grafts: four-year clinical results Arthritis Res Ther 11 2009 R33
36. C. Perka, O. Schultz, and M. Sittinger Chondrocyte transplantation in PGLA/polydioxanone fleece Orthopade 29 2000 112 119
37. N. Rotter, J. Aigner, and A. Naumann Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage J Biomed Mater Res 42 1998 347 356 (Pubitemid 28452509)
38. N. Baena, M. Guitart, and J.C. Ferreres Fetal and placenta chromosome constitution in 237 pregnancy losses Ann Genet 44 2001 83 88 (Pubitemid 32826001)
39. J.A. Bridge, P.S. Bhatia, and J.R. Anderson Biologic and clinical significance of cytogenetic and molecular cytogenetic abnormalities in benign and malignant cartilaginous lesions Cancer Genet Cytogenet 69 1993 79 90 (Pubitemid 23313843)
40. H. Chen, C.W. Yu, and R. Mulhern Chromosome preparations of chondrocytes cultured from human cartilages Am J Med Genet 6 1980 179 181 (Pubitemid 10004826)
41. W.H. Lee Jr., R.F. Hagerty, and H.L. Braid Measurements of cellular viability. A comparative study of neutral red and radioactive sulfate in the examination of the viability of cartilage Plast Reconstr Surg Transplant Bull 26 1960 280 285
42. M. Tamayo, A. Mosquera, and I. Rego Decreased length of telomeric DNA sequences and increased numerical chromosome aberrations in human osteoarthritic chondrocytes Mutat Res 708 2011 50 58
43. K. Broberg, J. Limon, and E. Palsson Clonal chromosome aberrations are present in vivo in synovia and osteophytes from patients with osteoarthritis Hum Genet 101 1997 295 298 (Pubitemid 28197523)
44. F. Mertens, E. Palsson, and A. Lindstrand Evidence of somatic mutations in osteoarthritis Hum Genet 98 1996 651 656 (Pubitemid 26387950)
45. E. van den Berg, T. Dijkhuizen, and S. Storkel Chromosomal abnormalities in non-neoplastic renal tissue Cancer Genet Cytogenet 85 1995 152 154 (Pubitemid 26030374)
46. R. Vanni, L. Cossu, and S. Licheri Atherosclerotic plaque as a benign tumor? Cancer Genet Cytogenet 47 1990 273 274 (Pubitemid 20226633)
47. G. Bardi, B. Johansson, and N. Pandis Trisomy 7 in nonneoplastic focal steatosis of the liver Cancer Genet Cytogenet 63 1992 22 24
48. B. Johansson, S. Heim, and N. Mandahl Trisomy 7 in nonneoplastic cells Genes Chromosomes Cancer 6 1993 199 205 (Pubitemid 23111753)
49. N. Bukvic, M. Gentile, and F. Susca Sex chromosome loss, micronuclei, sister chromatid exchange and aging: a study including 16 centenarians Mutat Res 498 2001 159 167 (Pubitemid 32973671)
50. K. Broberg, S. Toksvig-Larsen, and A. Lindstrand Trisomy 7 accumulates with age in solid tumors and non-neoplastic synovia Genes Chromosomes Cancer 30 2001 310 315 (Pubitemid 32126210)
51. R. Williams, I.M. Khan, and K. Richardson Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage PLoS One 5 2010 e13246
52. R. de la Fuente, J.L. Abad, and J. Garcia-Castro Dedifferentiated adult articular chondrocytes: a population of human multipotent primitive cells Exp Cell Res 297 2004 313 328 (Pubitemid 38798396)