Differential microRNA expression analysis in blastocysts by whole mount in situ hybridization and reverse transcription quantitative polymerase chain reaction on laser capture microdissection samples

Analytical Biochemistry

Volumn 423, Issue 1, 2012, Pages 93-101

  Goossens, Karen ^a   Spiegelaere, Ward De ^b   Stevens, Mieke ^a   Burvenich, Christian ^a   Spiegeleer, Bart De ^a   Cornillie, Pieter ^a   Zeveren, Alex Van ^a   Soom, Ann Van ^a   Peelman, Luc ^a  

^a GHENT UNIVERSITY   (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Blastocyst; Laser capture microdissection; MicroRNA

Indexed keywords

MICRODISSECTION; RNA;

BLASTOCYST; COMPLEMENTARY TECHNIQUES; LASER CAPTURE MICRODISSECTION; MICRORNA EXPRESSION; MICRORNAS; QUANTITATIVE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; WHOLE-MOUNT IN SITU HYBRIDIZATIONS;

POLYMERASE CHAIN REACTION;

MESSENGER RNA; MICRORNA;

ARTICLE; BLASTOCYST; EMBRYO; IN SITU HYBRIDIZATION; INNER CELL MASS; LASER CAPTURE MICRODISSECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; WHOLE MOUNT IN SITU HYBRIDIZATION;

EID: 84857325597     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.005     Document Type: Article

References (45)

1. H. Niwa, Y. Toyooka, D. Shimosato, D. Strumpf, K. Takahashi, R. Yagi, J. Rossant, Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation, Cell 123 (2005) 917-929. (Pubitemid 41721036)
2. D. Strumpf, C.A. Mao, Y. Yamanaka, A. Ralston, K. Chawengsaksophak, F. Beck, J. Rossant, Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst, Development 132 (2005) 2093-2102. (Pubitemid 40744469)
3. E.W. Kuijk, L. Du Puy, H.T. Van Tol, C.H. Oei, H.P. Haagsman, B. Colenbrander, B.A. Roelen, Differences in early lineage segregation between mammals, Dev. Dyn. 237 (2008) 918-927. (Pubitemid 351542290)
4. N. Kirchhof, J.W. Carnwath, E. Lemme, K. Anastassiadis, H. Scholer, H. Niemann, Expression pattern of Oct-4 in preimplantation embryos of different species, Biol. Reprod. 63 (2000) 1698-1705.
5. D.K. Berg, C.S. Smith, D.J. Pearton, D.N. Wells, R. Broadhurst, M. Donnison, P.L. Pfeffer, Trophectoderm lineage determination in cattle, Dev. Cell 20 (2011) 244-255.
6. Y. Tay, J. Zhang, A.M. Thomson, B. Lim, I. Rigoutsos, MicroRNAs to Nanog, Oct4, and Sox2 coding regions modulate embryonic stem cell differentiation, Nature 455 (2008) 1124-1128.
7. Y. Wang, S. Baskerville, A. Shenoy, J.E. Babiarz, L. Baehner, R. Blelloch, Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation, Nat. Genet. 40 (2008) 1478-1483.
8. R.L. Gardner, M.H. Johnson, An investigation of inner cell mass and trophoblast tissues following their isolation from the mouse blastocyst, J. Embryol. Exp. Morphol. 28 (1972) 279-312.
9. N. Tanaka, T. Takeuchi, Q.V. Neri, E.S. Sills, G.D. Palermo, Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model, J. Transl. Med. 4 (2006) 20.
10. D. Solter, B.B. Knowles, Immunosurgery of mouse blastocyst, Proc. Natl. Acad. Sci. U.S.A. 72 (1975) 5099-5102.
11. M. Ozawa, P.J. Hansen, A novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting, Fertil. Steril. 95 (2011) 799-802.
12. J. Adjaye, J. Huntriss, R. Herwig, A. BenKahla, T.C. Brink, C. Wierling, C. Hultschig, D. Groth, M.L. Yaspo, H.M. Picton, R.G. Gosden, H. Lehrach, Primary differentiation in the human blastocyst: comparative molecular portraits of inner cell mass and trophectoderm cells, Stem Cells 23 (2005) 1514-1525. (Pubitemid 41741000)
13. G. Cauffman, H. Van de Velde, I. Liebaers, A. Van Steirteghem, DAZL expression in human oocytes, preimplantation embryos, and embryonic stem cells, Mol. Hum. Reprod. 11 (2005) 405-411. (Pubitemid 41418064)
14. D. Piette, M. Hendrickx, E. Willems, C.R. Kemp, L. Leyns, An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies, Nat. Protoc. 3 (2008) 1194-1201.
15. M.M. Hossain, N. Ghanem, M. Hoelker, F. Rings, C. Phatsara, E. Tholen, K. Schellander, D. Tesfaye, Identification and characterization of miRNAs expressed in the bovine ovary, BMC Genomics 10 (2009) 443.
16. W.S. Abd El Naby, T.H. Hagos, M.M. Hossain, D. Salilew-Wondim, A.Y. Gad, F. Rings, M.U. Cinar, E. Tholen, C. Looft, K. Schellander, M. Hoelker, D. Tesfaye, Expression analysis of regulatory microRNAs in bovine cumulus oocyte complex and preimplantation embryos, Zygote (2011) 1-21.
17. D. Sweetman, T. Rathjen, M. Jefferson, G. Wheeler, T.G. Smith, G.N. Wheeler, A. Munsterberg, T. Dalmay, FGF-4 signaling is involved in mir-206 expression in developing somites of chicken embryos, Dev. Dyn. 235 (2006) 2185-2191. (Pubitemid 44205052)
18. D. Sweetman, K. Goljanek, T. Rathjen, S. Oustanina, T. Braun, T. Dalmay, A. Munsterberg, Specific requirements of MRFs for the expression of muscle specific microRNAs, miR-1, miR-206, and miR-133, Dev. Biol. 321 (2008) 491-499.
19. X. He, Y.L. Yan, A. DeLaurier, J.H. Postlethwait, Observation of miRNA gene expression in zebrafish embryos by in situ hybridization to microRNA primary transcripts, Zebrafish 8 (2011) 1-8.
20. W. De Spiegelaere, P. Cornillie, M. Van Poucke, L. Peelman, C. Burvenich, W. Van den Broeck, Quantitative mRNA expression analysis in kidney glomeruli using microdissection techniques, Histol. Histopathol. 26 (2011) 267-275.
21. M. Filliers, W. De Spiegelaere, L. Peelman, K. Goossens, C. Burvenich, L. Vandaele, P. Cornillie, A. Van Soom, Laser capture microdissection for gene expression analysis of inner cell mass and trophectoderm from blastocysts, Anal. Biochem. 408 (2011) 169-171.
22. L. Vandaele, B. Mateusen, D. Maes, A. de Kruif, A. Van Soom, Is apoptosis in bovine in vitro produced embryos related to early developmental kinetics and in vivo bull fertility?, Theriogenology 65 (2006) 1691-1703
23. K. Goossens, M. Van Poucke, A. Van Soom, J. Vandesompele, A. Van Zeveren, L.J. Peelman, Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos, BMC Dev. Biol. 5 (2005) 27.
24. S. Lefever, J. Vandesompele, F. Speleman, F. Pattyn, RTPrimerDB: the portal for real-time PCR primers and probes, Nucleic Acids Res. 37 (2009) D942-D945.
25. S.A. Bustin, V. Benes, J.A. Garson, J. Hellemans, J. Huggett, M. Kubista, R. Mueller, T. Nolan, M.W. Pfaffl, G.L. Shipley, J. Vandesompele, C.T. Wittwer, The MIQE guidelines: minimum information for publication of quantitative realtime PCR experiments, Clin. Chem. 55 (2009) 611-622.
26. J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes, Genome Biol. 3 (2002). RESEARCH0034.
27. J. Hellemans, G. Mortier, A. De Paepe, F. Speleman, J. Vandesompele, qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data, Genome Biol. 8 (2007) R19.
28. G. Obernosterer, J. Martinez, M. Alenius, Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections, Nat. Protoc. 2 (2007) 1508-1514. (Pubitemid 46952329)
29. V. Girish, A. Vijayalakshmi, Affordable image analysis using NIH Image/ImageJ, Indian J. Cancer 41 (2004) 47. (Pubitemid 38623932)
30. M.G. Forero, A.R. Learte, S. Cartwright, A. Hidalgo, DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila, PLoS One 5 (2010) e10557.
31. V. Benes, M. Castoldi, Expression profiling of microRNA using real-time quantitative PCR: How to use it and what is available, Methods 50 (2010) 244-249.
32. D. Ibberson, V. Benes, M.U. Muckenthaler, M. Castoldi, RNA degradation compromises the reliability of microRNA expression profiling, BMC Biotechnol. 9 (2009) 102.
33. M.L. Cox, S.M. Eddy, Z.S. Stewart, M.R. Kennel, M.Z. Man, J.D. Paulauskis, R.W. Dunstan, Investigating fixative-induced changes in RNA quality and utility by microarray analysis, Exp. Mol. Pathol. 84 (2008) 156-172.
34. M. Cummings, C.V. McGinley, N. Wilkinson, S.L. Field, S.R. Duffy, N.M. Orsi, A robust RNA integrity-preserving staining protocol for laser capture microdissection of endometrial cancer tissue, Anal. Biochem. 416 (2011) 123-125.
35. K. Goossens, A. Van Soom, M. Van Poucke, L. Vandaele, J. Vandesompele, A. Van Zeveren, L.J. Peelman, Identification and expression analysis of genes associated with bovine blastocyst formation, BMC Dev. Biol. 7 (2007) 64.
36. S.A. Degrelle, E. Campion, C. Cabau, F. Piumi, P. Reinaud, C. Richard, J.P. Renard, I. Hue, Molecular evidence for a critical period in mural trophoblast development in bovine blastocysts, Dev. Biol. 288 (2005) 448-460. (Pubitemid 41790755)
37. D. Pant, C.L. Keefer, Expression of pluripotency-related genes during bovine inner cell mass explant culture, Cloning Stem Cells 11 (2009) 355-365.
38. P. Mestdagh, T. Feys, N. Bernard, S. Guenther, C. Chen, F. Speleman, J. Vandesompele, High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA, Nucleic Acids Res. 36 (2008) e143.
39. T. Donnem, K. Eklo, T. Berg, S.W. Sorbye, K. Lonvik, S. Al-Saad, K. Al-Shibli, S. Andersen, H. Stenvold, R.M. Bremnes, L.T. Busund, Prognostic impact of MiR-155 in non-small cell lung cancer evaluated by in situ hybridization, J. Transl. Med. 9 (2011) 6.
40. I. Faraoni, F.R. Antonetti, J. Cardone, E. Bonmassar, miR-155 gene: a typical multifunctional microRNA, Biochim. Biophys. Acta 1792 (2009) 497-505.
41. R.X. Leng, H.F. Pan, W.Z. Qin, G.M. Chen, D.Q. Ye, Role of microRNA-155 in autoimmunity, Cytokine Growth Factor Rev. 22 (2011) 141-147.
42. M. Kamata, M. Liang, S. Liu, Y. Nagaoka, I.S. Chen, Live cell monitoring of hiPSC generation and differentiation using differential expression of endogenous microRNAs, PLoS One 5 (2010) e11834.
43. N. Pottier, T. Maurin, B. Chevalier, M.P. Puissegur, K. Lebrigand, K. Robbe-Sermesant, T. Bertero, C.L.L. Cardenas, E. Courcot, G. Rios, S. Fourre, J.M. Lo-Guidice, B. Marcet, B. Cardinaud, P. Barbry, B. Mari, Identification of keratinocyte growth factor as a target of microRNA-155 in lung fibroblasts: implication in epithelial-mesenchymal interactions, PLoS One 4 (2009) e6718.
44. F. Louafi, R.T. Martinez-Nunez, T. Sanchez-Elsner, MicroRNA-155 targets SMAD2 and modulates the response of macrophages to transforming growth factor-β, J. Biol. Chem. 285 (2010) 41328-41336.
45. E. Massabbal, S. Parveen, D.L. Weisoly, D.M. Nelson, S.D. Smith, M. Fant, PLAC1 expression increases during trophoblast differentiation: evidence for regulatory interactions with the fibroblast growth factor-7 (FGF-7) axis, Mol. Reprod. Dev. 71 (2005) 299-304. (Pubitemid 40770383)