Comment to: "Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro" by Z. Ren et al. Exp. Cell Res. 317 (2011) 2950-2957. Spontaneous transformation of mesenchymal stem cells in culture: Facts or fiction?

Experimental Cell Research

Volumn 318, Issue 5, 2012, Pages 441-443

  Torsvik, Anja ^a   Røsland, Gro V ^a   Bjerkvig, Rolf ^a,b  

^a UNIVERSITY OF BERGEN   (Norway)

^b LUXEMBOURG INSTITUTE OF HEALTH   (Luxembourg)

Author keywords

Cross contamination; DNA fingerprinting; Mesenchymal stem cells; Spontaneous transformation; STR analysis; Transformed mesenchymal cell

Indexed keywords

BETA CATENIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; VIMENTIN;

AMPLICON; CANCER CELL; CANCER CELL CULTURE; CELL CULTURE; CELL STRUCTURE; CELL TRANSFORMATION; DNA FINGERPRINTING; DNA POLYMORPHISM; DNA SEQUENCE; GENE EXPRESSION; GENE LOCUS; GENETIC VARIABILITY; HAPLOTYPE; HUMAN; IN VITRO STUDY; MACACA; MESENCHYMAL STEM CELL; NONHUMAN; NOTE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

MACACA;

EID: 84857061727     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2011.12.009     Document Type: Editorial

References (21)

1. Ren Z., Wang J., Zhu W., Guan Y., Zou C., Chen Z., Zhang Y.A. Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro. Exp. Cell Res. 2011, 317:2950-2957.
2. Rosland G.V., Svendsen A., Torsvik A., Sobala E., McCormack E., Immervoll H., Mysliwietz J., Tonn J.C., Goldbrunner R., Lonning P.E., Bjerkvig R., Schichor C. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009, 69:5331-5339.
3. Rubio D., Garcia-Castro J., Martin M.C., de la Fuente R., Cigudosa J.C., Lloyd A.C., Bernad A. Spontaneous human adult stem cell transformation. Cancer Res. 2005, 65:3035-3039.
4. de la Fuente R., Bernad A., Garcia-Castro J., Martin M.C., Cigudosa J.C. Retraction: spontaneous human adult stem cell transformation. Cancer Res. 2010, 70:6682.
5. Torsvik A., Rosland G.V., Svendsen A., Molven A., Immervoll H., McCormack E., Lonning P.E., Primon M., Sobala E., Tonn J.C., Goldbrunner R., Schichor C., Mysliwietz J., Lah T.T., Motaln H., Knappskog S., Bjerkvig R. Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track - letter. Cancer Res. 2010, 70:6393-6396.
6. O'Connor S.L., Blasky A.J., Pendley C.J., Becker E.A., Wiseman R.W., Karl J.A., Hughes A.L., O'Connor D.H. Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques. Immunogenetics 2007, 59:449-462.
7. Wang Y., Huso D.L., Harrington J., Kellner J., Jeong D.K., Turney J., McNiece I.K. Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture. Cytotherapy 2005, 7:509-519.
8. Burns J.S., Abdallah B.M., Guldberg P., Rygaard J., Schroder H.D., Kassem M. Tumorigenic heterogeneity in cancer stem cells evolved from long-term cultures of telomerase-immortalized human mesenchymal stem cells. Cancer Res. 2005, 65:3126-3135.
9. Serakinci N., Guldberg P., Burns J.S., Abdallah B., Schrodder H., Jensen T., Kassem M. Adult human mesenchymal stem cell as a target for neoplastic transformation. Oncogene 2004, 23:5095-5098.
10. Magdolen U., Schmitt M., Hildebrandt B., Diehl P., Schauwecker J., Saldamli B., Burgkart R., Tubel J., Gradinger R., Royer-Pokora B. Spontaneous in vitro transformation of primary human osteoblast-like cells. Cancer Genomics Proteomics 2010, 7:61-66.
11. Wu W., He Q., Li X., Zhang X., Lu A., Ge R., Zhen H., Chang A.E., Li Q., Shen L. Long-term cultured human neural stem cells undergo spontaneous transformation to tumor-initiating cells. Int. J. Biol. Sci. 2011, 7:892-901.
12. Boukamp P., Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 1988, 106:761-771.
13. Li H., Fan X., Kovi R.C., Jo Y., Moquin B., Konz R., Stoicov C., Kurt-Jones E., Grossman S.R., Lyle S., Rogers A.B., Montrose M., Houghton J. Spontaneous expression of embryonic factors and p53 point mutations in aged mesenchymal stem cells: a model of age-related tumorigenesis in mice. Cancer Res. 2007, 67:10889-10898.
14. Miura M., Miura Y., Padilla-Nash H.M., Molinolo A.A., Fu B., Patel V., Seo B.M., Sonoyama W., Zheng J.J., Baker C.C., Chen W., Ried T., Shi S. Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation. Stem Cells 2006, 24:1095-1103.
15. Zhou Y.F., Bosch-Marce M., Okuyama H., Krishnamachary B., Kimura H., Zhang L., Huso D.L., Semenza G.L. Spontaneous transformation of cultured mouse bone marrow-derived stromal cells. Cancer Res. 2006, 66:10849-10854.
16. Furlani D., Li W., Pittermann E., Klopsch C., Wang L., Knopp A., Jungebluth P., Thedinga E., Havenstein C., Westien I., Ugurlucan M., Li R.K., Ma N., Steinhoff G. A transformed cell population derived from cultured mesenchymal stem cells has no functional effect after transplantation into the injured heart. Cell Transplant. 2009, 18:319-331.
17. Ahmadbeigi N., Shafiee A., Seyedjafari E., Gheisari Y., Vassei M., Amanpour S., Amini S., Bagherizadeh I., Soleimani M. Early spontaneous immortalization and loss of plasticity of rabbit bone marrow mesenchymal stem cells. Cell Prolif. 2011, 44:67-74.
18. Jeong J.O., Han J.W., Kim J.M., Cho H.J., Park C., Lee N., Kim D.W., Yoon Y.S. Malignant tumor formation after transplantation of short-term cultured bone marrow mesenchymal stem cells in experimental myocardial infarction and diabetic neuropathy. Circ. Res. 2011, 108:1340-1347.
19. A.T.C.C.S.D.O.W.ASN-0002 Cell line misidentification: the beginning of the end. Nat. Rev. Cancer 2010, 10:441-448.
20. Garcia S., Martin M.C., de la Fuente R., Cigudosa J.C., Garcia-Castro J., Bernad A. Pitfalls in spontaneous in vitro transformation of human mesenchymal stem cells. Exp. Cell Res. 2010, 316:1648-1650.
21. Cooper J.K., Sykes G., King S., Cottrill K., Ivanova N.V., Hanner R., Ikonomi P. Species identification in cell culture: a two-pronged molecular approach. In Vitro Cell Dev. Biol. Anim. 2007, 43:344-351.